Enumeration and Characterization of Mesenchymal Stem Cells from Agedependent Human Dental Tissue

Abstract

Age-dependent chronic and aggressive periodontitis patients need renewal of soft and hard tissue like periodontal ligament, gingiva, cementum and alveolar bone. Hence, multipotent-Mesenchymal Stromal Cells (MSCs) of different dental origin like Dental Pulp (DP); Periodontal Ligament (PDL); Gingiva; Apical Papilla (AP) may play important role in regeneration of periodontal tissue. For the accomplishment of this objective, we standardized and validated the manufacturing process of dental-MSCs sources like DP, PDL, AP and gingiva from the subjects ranging from the age of 13 to 60 years. As per, International guidelines, we isolated and enumerated plastic-adherent MSCs at different time-points from different dental sources, phenotypically characterized these cells and differentiated them into osteogenic, adipogenic, and chondrogenic cells. Importantly, in subjects from 13-31 years copious DP gave an appropriate amount of MSCs. While, between the age group 50-60 years negligible amount of DP was extracted, which did not provide any MSCs. However, PDL and gingiva produced a good amount of MSCs from 13-31 years, but in 50-60 years the yield was not good. Hence, age does affect MSCs number which is the reason of delayed healing in older age group subjects compared to younger subjects.

In vitro histomorphometric comparison of dental pulp tissue in different teeth

“…In the literature, reports on morphometric and histological measurements of the DP regions among temporary and permanent teeth are quite limited. Besides, pulp weight is a parameter that few authors have considered to obtain DPSCs (Alsulaimani et al, 2016; Kellner et al, 2014; Singh et al, 2016). Our research group considers that DP weight could be important as a starting point for the isolation of DPSCs.…”