Enumeration of Soybean‐Associated Rhizobia with Quantitative Real‐Time Polymerase Chain Reaction

Furseth, Branden J.; Conley, Shawn P.

doi:10.2135/cropsci2010.03.0141

Cited by 11 publications

(13 citation statements)

References 21 publications

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“…Despite these limitations of the procedure, recovery of rhizobia by plant infection is usually the only way of positively identifying and enumerating them from nonsterile media like soil. Molecular methods are now being developed (Furseth et al, 2010) and, hopefully, they will be faster and more precise than the MPN method.…”

Section: Discussionmentioning

confidence: 99%

Intensification of Field Pea Production: Impact on Soil Microbiology

et al. 2012

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Th e economic and environmental benefi ts of including grain legumes in crop rotations may tempt farmers to grow them more frequently than recommended, resulting in potential changes to soil chemical, physical, and biological properties. We investigated the eff ects of increasing the frequency of fi eld pea (Pisum sativum L.) (P) in a wheat (Triticum aestivum L.) (W)-based cropping system on soil microbial biomass C (MBC), β-glucosidase enzyme activity, bacterial diversity, and populations of Rhizobium leguminosarum bv. viceae in the last 3 yr of a 13-yr fi eld study. Th e treatments consisted of three rotations: P-P, W-P, and W-W-P. Fertilizer N at 5, 20, and 40 kg N ha -1 was applied to pea in P-P and W-P rotations to examine the role of starter N. Soil MBC and diversity were lower in P-P than pea rotated with wheat, presumably due to reduced amounts and diversity of C inputs under P-P. Th ese reductions in soil MBC and diversity probably further reduced fi eld pea growth and grain yields through reduced nutrient cycling. In fi eld pea, β-glucosidase activity increased with increasing N, suggesting that N was limiting the capacity of soil microorganisms to recycle nutrients from organic materials (including crop residues). Populations of soil rhizobia were not aff ected by treatment. Wheat grown aft er pea in the 3-yr W-W-P rotation had greater MBC and β-glucosidase activity than that in the W-P rotation, indicating the importance of long rotations. Th erefore, pea monoculture reduced soil microbial quality, with adverse eff ects on nutrient cycling.

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Section: Discussionmentioning

confidence: 99%

Intensification of Field Pea Production: Impact on Soil Microbiology

et al. 2012

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“…DNA was extracted using MoBio Lahoratories' (Carlshad, CA) UltraClean-htp 96 Well Soil DNA Isolation Kit. Because different DNA extraction procedures produce slightly different products, the qPCR method was recalihrated for the 96-^vell extraction kit using the same soil samples and procedure as Furseth et al, (2010), Data were analyzed using the MIXED Procedure of SAS version 9,2 (SAS Institute, 2008), Due to high variation in yield between 2009 and 2010, the effects of year and location were merged into one environment variable for data analysis. Yield variation hetween years can he attributed to variable environmental conditions: warmer than average in 2010 with timely rains and a cooler, drier 2009 growing season.…”

Section: Methodsmentioning

confidence: 99%

“…Soil samples, consisting of fi ve 2.5-cm cores to a depth of 25 cm, were collected from each plot at the time of planting for the determination of preplant soil rhizobia populations using quantitative polymerase chain reaction (qPCR) (Furseth et al, 2010). Care was taken to avoid inoculated seeds by collecting soil samples from between the rows.…”

Section: Methodsmentioning

confidence: 99%

“…DNA was extracted using MoBio Laboratories' (Carlsbad, CA) UltraClean-htp 96 Well Soil DNA Isolation Kit. Because diff erent DNA extraction procedures produce slightly diff erent products, the qPCR method was recalibrated for the 96-well extraction kit using the same soil samples and procedure as Furseth et al (2010). Data were analyzed using the MIXED Procedure of SAS version 9.2 (SAS Institute, 2008).…”

Section: Methodsmentioning

confidence: 99%

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Soybean Response to Soil Rhizobia and Seed‐applied Rhizobia Inoculants in Wisconsin

Furseth

Conley

2012

Crop Science

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Seed-applied rhizobia inoculants can be used to promote biological nitrogen fixation in soybean [Glycitie max (L.) Merr.] production, yet their efficacy remains highly variable. The goal of this study was to improve the predictability of a positive soybean seed yield and quality response to inoculation by (i) characterizing inoculant product response in multiple environments, and (ii) quantifying the response of soybean to indigenous soil rhizobia populations. Two inoculants and a control were tested on three soybean varieties at nine locations in Wisconsin during 2009 and 2010. Soil samples were collected from each plot for the quantification of soil rhizobia. Seed yield, protein, and oil did not respond to inoculation across all 18 environments (P = 0.15, 0.38, and 0.30, respectively). Three environments responded positively to inoculation (P < 0.05), while the remainder showed no response. Yield of the control treatment was positively correlated with the soil rhizobia population level across all environments (P = 0.005), while the inoculated treatments showed no response. These data indicate a yield advantage for inoculation at lower rhizobia populations; however, given the large range of response with only three of 18 environments showing a positive response, a rhizobia population threshold to aid in the decision to treat soybean seeds with a rhizobia inoculant cannot be determined.

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“…Molecular analyses are more and more frequently used for an accurate determination of the degree of B. japonicum proliferation. To verify the effectiveness of bacterial inoculations, and thus the number of bacteria in root nodules, quantitative technique real-time PCR (qPCR, quantitative Polymerase Chain Reaction] is used, which is DNA-based and it facilitates, in addition to identifying B. japonicum, determining the degree of root colonization by bacteria [28].…”

Section: Introductionmentioning

confidence: 99%

Response of Soybean (Glycine max (L.) Merrill) to Mineral Nitrogen Fertilization and Bradyrhizobium japonicum Seed Inoculation

2020

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A growing interest in soybean cultivation in Poland has been observed in the recent years, however it faces a lot of difficulties resulting from a poorly understood effectiveness of plant nitrogen fertilization and from the introduction of Bradyrhizobium japonicum to the environment. The aim of the study was to evaluate the consistency of response of two soybean cultivars to three different rates of mineral N fertilization and two seed inoculation treatments with B. japonicum in field conditions over four years regardless of previous B. japonicum presence in the soil. A highly-diversified-over-years rainfall and temperature in the growing season do not allow for a definite statement of the differences resulting from seed inoculation and mineral N fertilization applied separately or jointly in soybean. A high sensitivity of the nodulation process to rainfall deficits was noted, which resulted in a decreased amount of B. japonicum DNA measured in qPCR and dry matter of nodules. ‘Annushka’ demonstrated a higher yield of seeds and protein, higher plants and the 1st pod setting. ‘Aldana’, due to a significant decrease in plant density, produced a higher number of pods, seeds per pod and the 1000 seed weight per plant. Both cultivars responded with an increase in the seed yield after seed inoculation with HiStick, also with an application of 30 and 60 kg N, as well as with Nitragina with 60 kg N.

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Enumeration of Soybean‐Associated Rhizobia with Quantitative Real‐Time Polymerase Chain Reaction

Cited by 11 publications

References 21 publications

Intensification of Field Pea Production: Impact on Soil Microbiology

Intensification of Field Pea Production: Impact on Soil Microbiology

Soybean Response to Soil Rhizobia and Seed‐applied Rhizobia Inoculants in Wisconsin

Response of Soybean (Glycine max (L.) Merrill) to Mineral Nitrogen Fertilization and Bradyrhizobium japonicum Seed Inoculation

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