Enumeration of the absolute CD4 T‐lymphocyte count by cell‐bead assay

Abstract

Background: We have previously developed an alternative approach for undertaking absolute cell counting based upon flow-rate calibration using cell bead (FCB), in which cell bead (CB) can be used as a flow-rate calibration material for generating the absolute microparticle counts. Here, we extended our work of counting CD41 T-lymphocytes in HIV-infected blood samples with the FCB method.Methods: CD41 T-lymphocyte counts in EDTA blood samples from 30 healthy subjects and 80 HIV-1-infected patients were determined using TriTEST reagent. The absolute CD41 T-lymphocytes were measured by FCB, and the results were compared with the absolute counting by commercial latex bead (CLB) or with flow rate-based calibration method (FR). Statistical correlation and agreement were analyzed using linear regression and Bland-Altman analysis. Results: There was no significant difference in the absolute number of CD41 T-lymphocyte counts enumerated by FCB when compared with those two reference methods (CLB and FR). The absolute CD41 T-lymphocyte counts obtained from

Interleukin-17A-Induced Human Mesenchymal Stem Cells Are Superior Modulators of Immunological Function

“…T cells were stained for T cell activation or Treg markers and defined as described previously . The absolute number of positively stained cells was calculated using the allophycocyanin (APC)‐conjugated CaliBRITE Beads (BD Biosciences) . Induced iTregs were also further characterized for activation and functional markers.…”

Issue highlights—Clinical Cytometry (Cytometry Part B) January 2012

“…The identification and precise quantification of these subsets may have importance in patient follow‐up. This group previously described several novel cellular subsets and worked out standardized methods to enumerate relevant cell types in HIV‐infected patients (2).…”

LettersResponse From the Authors

“…Another difference is the focus in the Cytometry Part B publication on the diagnostic performance of the CB assay using blood samples selected from a population of HIV+ individuals. 1,2 Cost data, relevant to the CB-assay compared to LB and FR methods, are shown clearly in Table 2 of the LabMedicine publication. 1 The total cost of the CB assay is approximately half that of the CB and FR assays, while the analytical and clinical performance characteristics of the CB assay are comparable to those of the CB and FR assays.…”

“…1 The title and content of this publication appear very similar to a previously published article by many of these same authors. 2 Moreover, if the principal difference between these two publications is the emphasis on the issue of "affordability" in the LabMedicine publication, then this publication should have included a cost comparison between traditional methods for counting CD4+ lymphocytes vs the method discussed by the authors across different laboratory settings and a discussion of the internal and external quality control procedures, including cost, related to this putative "affordable" method. …”