2002
DOI: 10.1016/s0167-7012(01)00370-0
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Enumeration of total bacteria and bacteria with genes for proteolytic activity in pure cultures and in environmental samples by quantitative PCR mediated amplification

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Cited by 206 publications
(102 citation statements)
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References 29 publications
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“…The application of this Q-PCR method confirmed: (i) that OOB were numerous in soils, in similar proportions as other important functional groups e.g. the proteolytic bacteria (Bach et al, 2002) and (ii) that OOB relative abundance was linked to the operation of the oxalate-carbonate pathway as it increased with oxalate and carbonate contents. If the results are carefully analyzed by considering the frc/16S rDNA genes ratio, SybrGreen real-time PCR with the degenerate primers designed in this study was demonstrated to be a reliable and accurate method to monitor OOB density in pure cultures and in a complex environment.…”
Section: Abundance Of Soil Oxalate-oxidizing Bacteriasupporting
confidence: 54%
See 1 more Smart Citation
“…The application of this Q-PCR method confirmed: (i) that OOB were numerous in soils, in similar proportions as other important functional groups e.g. the proteolytic bacteria (Bach et al, 2002) and (ii) that OOB relative abundance was linked to the operation of the oxalate-carbonate pathway as it increased with oxalate and carbonate contents. If the results are carefully analyzed by considering the frc/16S rDNA genes ratio, SybrGreen real-time PCR with the degenerate primers designed in this study was demonstrated to be a reliable and accurate method to monitor OOB density in pure cultures and in a complex environment.…”
Section: Abundance Of Soil Oxalate-oxidizing Bacteriasupporting
confidence: 54%
“…It has been demonstrated that functional genes could be used as molecular markers to assess the diversity structure and the abundance of bacterial functional groups (Bach et al, 2002;Lejon et al, 2007;Nyyssönen et al, 2006;Lopez-Gutierrez et al, 2004;Selesi et al, 2007;Poly et al, 2008). To date, no equivalent tool was proposed for the oxalotrophic bacterial communities.…”
Section: Introductionmentioning
confidence: 99%
“…Quantitative assays are useful for determining the virulence mechanism(s) of pathogens, infection ability of insect vectors, and development of efficient management strategies (Alvarez 2004;Bach et al 2002). Since the efforts to isolate Las in pure culture have been unsuccessful, accurate quantification remains difficult.…”
Section: Introductionmentioning
confidence: 99%
“…Identification and detection systems based on real-time quantitative PCR (QPCR) have been described for several bacteria, including B. anthracis (1,2,13,22). These techniques are easy to design, simple to carry out, and commonly applied to monitor and detect the microbial status of sample specimens.…”
mentioning
confidence: 99%