Environment of the 5′-terminal nucleotide of the mRNA codon at the P and E sites of human ribosome: Crosslinking with pUUUGUU derivatives bearing a photoactivatable group at an uracil residue or 5′-phosphate

The 18S rRNA nucleotides close to the 80S ribosome template nucleotide adjacent to the A-site codon on the 3-end (i.e., the nucleotide in position +7 relative to the first nucleotide of the P-site codon) were identified using template-controlled chemical affinity ligation. For this purpose, used the photoreactive mRNA analogues with a perfluorophenylazido group attached through various linkers to the uridine C5,3'-terminal phosphate, or guanosine N7 were used. The position of the mRNA analogues on the ribosome was preset using tRNAPhe, which recognized the phenylalanine codon directed to the P-site. An analysis of the rRNAs isolated from the irradiated complexes of 80S ribosomes showed that all the analogues are almost equally ligated to the 18S rRNA nucleotides we attributed to the A-site codon environment: namely, to nucleotides A1823, A1824, and A1825 of the 3'-minidomain and to the 620-630 fragment of the 18S rRNA 5'-domain. In addition, we identified a new component of the mRNA binding site of human ribosomes, nucleotide C1698 belonging to the 18S rRNA 3-minidomain, using analogues bearing a perfluorophenylazido group on uridine and guanine residues. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 3; see also http://www.maik.ru.

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Template Location on the Human Ribosome: Environment of the mRNA Nucleotide Adjacent to the A-Site Codon on the 3′-Side

Demeshkina

Styazhkina

Bulygin

et al. 2005

Russ J Bioorg Chem

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Positioning of mRNA codons with respect to 18S rRNA at the P and E sites of human ribosome

Demeshkina

Laletina

Meschaninova

et al. 2003

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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Graifer

Карпова

2001

Molecular Biology

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Environment of the 5′-terminal nucleotide of the mRNA codon at the P and E sites of human ribosome: Crosslinking with pUUUGUU derivatives bearing a photoactivatable group at an uracil residue or 5′-phosphate

Cited by 7 publications

References 10 publications

Template Location on the Human Ribosome: Environment of the mRNA Nucleotide Adjacent to the A-Site Codon on the 3′-Side

Template Location on the Human Ribosome: Environment of the mRNA Nucleotide Adjacent to the A-Site Codon on the 3′-Side

Positioning of mRNA codons with respect to 18S rRNA at the P and E sites of human ribosome

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