Abstract:In Africa, donkeys have significant contribution in traction operation, but there is little research related to workload and their welfare. The objective of this work was to examine the effect of various loads on donkeys' physiological and behavioural responses to evaluate welfare. Donkeys weighing of 132-172 kg (159 ± 11 kg) and totally 20 were used for the experiment. The experimental design included two wheeled cart × seven load class (an increment of 100 kg) with five repetitions each, and the travelled distance was 2 km. Travelled time, behavioural occurrences and physiological responses were recorded. Statistical analysis was conducted using SAS (version 9.4) software programme. When loads were 500-700 kg, heavy and rapid panting and falling down occurred frequently. When loads increased successively and reached 500, 600 and 700 kg, speed started declining from 3.68 km/h to 3.5, 2.94 and 2.54 km/h, respectively, indicating that speed and applied loads are inversely correlated (P < 0.001). Heart rate was 129 ± 11 bpm at 700 kg, and in respect to resting state, donkeys' heart rate elevated by a factor of up to 3. As the load exceeded 400 kg, the speed declined significantly, and behavioural occurrences like heavy panting and falling down confirmed this. This leads to the conclusion that donkeys could pull about 2.7 times of their live weight. But if they have continuous and long working hours (about 6 h), it is recommended to keep load of two wheeled cart about double of donkeys live weight so as to safe guard donkeys' welfare.