Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro

Cao, Shan; Xu, Wei; Lou, Qin-Qin; Zhang, Yinfeng; Zhao, Yaxian; Wei, Wuji; Qin, Zhan-Fen

doi:10.1016/j.tiv.2013.12.003

Cited by 3 publications

(4 citation statements)

References 52 publications

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“…Therefore, we used a large number of recently captured specimens. Nevertheless, we observed individual variability in the sensitivity to the steroids tested, similar to those observed for X. laevis oocytes (Cao et al , 2014). This variability may result from different levels of endogenous gonadotropins that impact on ovarian physiology and on subsequent oocyte sensitivity to steroid-induced GVBD, as observed by LaMarca et al (1985) in X. laevis oocytes.…”

Section: Resultssupporting

confidence: 88%

“…In our experiments the sensitivity of R. arenarum follicles to GVBD varied between frogs in a similar way to the results obtained with X. laevis oocytes (Pickford & Morris, 1999; Haccard et al , 2012; Cao et al , 2014). We found that in oocytes and follicles the response to androgens varied among adult R. arenarum ovaries in the experiments carried out for 3 years.…”

Section: Resultssupporting

confidence: 86%

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In vitro steroid-induced meiosis in Rhinella arenarum oocytes: role of pre-MPF activation

Torres

Bühler

Zelarayán

2015

Zygote

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In this work we showed the relationship between seasonal periods and the response of R. arenarum follicles and oocytes to different steroids. Using in vitro germinal vesicle breakdown (GVBD) assays, we demonstrated that P4 is the main steroid capable of inducing maturation in R. arenarum oocytes and follicles. In the second part of this work we showed that androgens can activate pre-maturation promoting factors (pre-MPFs) such as P4, by cytoplasm microinjection experiments. The results indicated that the steroids assayed induced oocyte and follicle maturation in a dose- and time-dependent manner. In oocytes, P4 was the most efficient steroid as a maturation inducer (EC50 of the reproductive period, 6 nM, EC50 of the non-reproductive period ≅ 30 nM). Androgens (DHEA, dehydroepiandrosterone; T, testosterone; and AD, androstenedione) were less efficient maturation inducers than P4 (EC50 reproductive period ≅ 50, 120 and 600 nM respectively). Similar results were obtained with intact follicles in both seasonal periods. Although the response of follicles to the different androgens was variable, in no case was it above the above the response induced by P4. Independently of the season, oocytes and follicles incubated in P4, P5 and T underwent GVBD after 6-10 h while oocytes and follicles incubated in DHEA and AD matured more slowly. Furthermore, we demonstrated that microinjection of mature cytoplasm from androgen-treated oocytes is sufficient to promote GVBD in immature recipient oocytes (DHEA, 57 ± 12%; AD, 60 ± 8%; T, 56 ± 13%). Thus, androgens such as DHEA, T and AD are as competent as P4 to activate pre-MPF.

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Section: Resultssupporting

confidence: 88%

Section: Resultssupporting

confidence: 86%

In vitro steroid-induced meiosis in Rhinella arenarum oocytes: role of pre-MPF activation

Torres

Bühler

Zelarayán

2015

Zygote

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“…Androgens, most notably testosterone, have been shown to induce GVBD (Cao et al, ; Lutz et al, ; Lutz et al, ) and have been hypothesized to be the physiological regulators of oocyte maturation in vivo (Lutz et al, ). Lutz et al () induced a marked increase in androgen levels, but not in progesterone levels female X. laevis serum following injection with the injection of human chorionic gonadotropin, commonly used to induce superovulation and breeding in the laboratory (Bantle et al, ; ASTM, ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of germinal vesicle breakdown in Xenopus oocytes in vitro by a series of substituted glycol ethers

Fort

Mathis

Guiney

et al. 2017

J of Applied Toxicology

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A 24 hour in vitro Xenopus oocyte maturation (germinal vesicle breakdown [GVBD]) assay developed by Pickford and Morris (Environmental Health Perspectives, 1999, 107, 285-292) was used to screen a series of substituted glycol ethers (GEs). Substituted GEs included: ethylene glycol monomethyl ether (EGME); EG monoethyl ether (EGEE); EG monopropyl ether (EGPE); EG monobutyl ether (EGBE); EG monohexyl ether (EGHE); diethylene glycol monomethyl ether (DGME); triethylene glycol monomethyl ether (TGME); ethylene glycol monophenyl ether (EGPhE); EG monobenzyl ether (EGBeE); EG diphenyl ether (EGDPhE); and propylene glycol monophenyl ether (PGPhE). The GEs inhibited progesterone- or androstenedione-induced GVBD with the following relative potency: EGPhE > PGPhE > EGME >> EGEE ≥ EGBeE > EGPE >> EGBE >EGHE > EGDPhE >> DGME ≥ TGME, or EGPhE >> PGPhE >> EGBeE > EGDPhE > EGEE > EGME > EGPE > EGBE, EGHE, DGME and TGME, respectively. Further, [ H]progesterone or [ H]androstenedione binding affinities to the oocyte plasma membrane progesterone receptor (OMPR) or classical androgen receptor (AR) were: EGME > EGPhE ≥ PGPhE ≥ EGEE > EGBeE >> EGPE >> EGBE ≥ EGHE > EGDPhE, TGME, and DGME, or EGPhE > PGPhE >> EGBeE > EGDPhE >> EGEE ≥ EGME >> EGPE, EGBE, and EGHE > DGME and TGME, respectively. Binary joint mixture studies with the GVBD model using flutamide (AR antagonist) and EGPhE indicated that flutamide/EGPhE mixture acted in a concentration additive manner. The effects of substituted GE series, however, may be mediated through the OMPR; the potency of EGPhE may be the result of bimodal inhibition of both the OMPR and AR pathways.

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“…Xenopus laevis Germinal vesicle breakdown (Cao et al, 2014) Estrogen receptor agonists and antagonists 17α-Ethinylestradiol Fathead Minnow Significant reduction in number of eggs (Pawlowski et al, 2004) Beta presence at higher concentrations in water bodies leading to an array of detrimental effects on all aquatic organisms (Kovalakova et al, 2020). NSAIDs have a widespread use and their bioaccumulation across various organisms lead to their eventual consumption by scavenger avians like vultures where it causes renal toxicity (He et al, 2017).…”

Section: Nandrolone Estersmentioning

confidence: 99%

Aptasensors in environmental forensics: Tracking the silent killers

Dube

Satish

Rawtani

2023

WIREs Forensic Science

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Since its inception, environmental forensics has observed expansion of its horizons across several threats to life forms, be it pertaining to wildlife trafficking or tracking the release of effluents containing pharmaceutical drugs, toxins, pesticides, heavy metals, pathogenic microbes, and microplastics. With the substantial growth of pharmaceutical industry over the past few decades, especially with respect to therapeutic personal care products and over the counter drugs, a new threat to environment has emerged from pharmaceutical toxicity.Due to the recalcitrant nature and poor wastewater treatment practices related to these pollutants, they leach into different strata of ecosystem like aquatic, terrestrial, and atmospheric systems. These drugs pose a threat to several life forms even at trace concentrations. This article aims at providing a profound perspective by exhaustively reviewing several classes of pharmaceutical drugs that are silently threatening the wildlife and environment. The sensitive, specific, and rapid detection of such drugs menacing the environment and wildlife is pertinent and can be achieved through aptamer-based biosensors known as aptasensors. These are a rising class of biosensors with vast application potential in numerous fields including environmental and wildlife forensics. This article also highlights the gap in research in the availability of rapid sensors for trace detection of drugs in environment hence encouraging researchers to explore the under-explored potential of aptasensors in environmental forensics. Highly sensitive and selective detection of such drugs is crucial in narrowing down the origin of such pollutants so as to aid the regulatory authorities in enforcing control over their release into environment.

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Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro

Cited by 3 publications

References 52 publications

In vitro steroid-induced meiosis in Rhinella arenarum oocytes: role of pre-MPF activation

In vitro steroid-induced meiosis in Rhinella arenarum oocytes: role of pre-MPF activation

Inhibition of germinal vesicle breakdown in Xenopus oocytes in vitro by a series of substituted glycol ethers

Aptasensors in environmental forensics: Tracking the silent killers

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