Environmental Applications of Electromembrane Extraction: A Review

Shi, Linping; Chen, Mantang; Zhao, Ge; Wang, Xiaoyu; Fan, Meijuan; Liu, Ruihong; Xie, Fuwei

doi:10.3390/membranes13080705

Cited by 9 publications

(1 citation statement)

References 103 publications

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“…Presently, EME is mainly performed for the separation of small molecules, such as metal ions, clinical medicine, and drugs. − EME of nucleic acid targets (which are acidic, polar, and larger in molecular weight) has not been achieved. Oligonucleotides are defined as linear polymers of nucleotides, some of which are important functional nucleic acids, including primers, microribonucleic acids (miRNAs), probes, etc.…”

Section: Introductionmentioning

confidence: 99%

Confined Gel-Electromembrane Extraction of Oligonucleotides

Liu,

Liu

et al. 2024

Anal. Chem.

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Gel-electromembrane extraction (G-EME) is an increasingly popular green variant of electromembrane extraction (EME). However, the electroendosmosis (EEO) flow associated with G-EME greatly limits the development of this technology. To address this challenge, the current study proposed the concept of confined G-EME (CG-EME), and a three-dimensional-printed modular device was elaborately designed to realize this concept. The device blocked the EEO flow by limiting the volume of the sample compartment. Moreover, the mesh structure at the bottom of the extraction module helps to prepare thin and stable gel films, which enhance the electromigration driving force and shorten the migration path. In addition, polar oligonucleotides, a nucleic acid analyte, were extracted for the first time to prove the concept of CG-EME. After optimization, 62% of the oligonucleotides were extracted at 50 V voltage for 15 min using a 3 mm thick agarose (3%) gel film. Finally, the application capability of CG-EME was further demonstrated by recovering DNA primers and isolating disease biomarkers (miRNA-181b) from real samples. In combination with CG-EME and quantitative polymerase chain reaction (qPCR) analysis, the upregulation of miRNA-181b expression in the peripheral blood of patients with schizophrenia was observed. In conclusion, this study proposes CG-EME to diminish EEO and push EME into the clinical field to isolate nucleic acid biomarkers, which will greatly expand the application scenarios of this emerging technology.

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Section: Introductionmentioning

confidence: 99%

Confined Gel-Electromembrane Extraction of Oligonucleotides

Liu,

Liu

et al. 2024

Anal. Chem.

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Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance

Orlando,

Dvořák,

Kubáň

2024

Electrophoresis

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Electroextraction (EE) encompasses a range of sample preparation methods whose effectiveness, selectivity, and efficiency are significantly influenced by the physical‐chemical characteristics of analytes, samples, and instrumental conditions. This article explores, for the first time, various strategies aimed at enhancing the extraction efficiency of a recent approach of EE utilizing a paper point (PP) combined with a hollow fiber (HF) (abbreviated as PP‐HF‐EE) to extract various cationic and anionic model compounds from water samples. The study also explores, experimentally, the impact of agitation, organic filter composition, PP diameter, and PP brand on extraction performance, and proves that all these factors are quite important, especially when digital image analysis is utilized for determination. Furthermore, this work demonstrates the ease and feasibility of simultaneously extracting cations and anions using PP‐HF‐EE and proposes a straightforward method to enhance analyte concentration on the vertex of the PP through a base‐to‐vertex focusing. Lastly, it is demonstrated, using capillary electrophoresis coupled to a UV‐Vis detector, that for PP‐HF‐EE, the extraction efficiency and pre‐concentration factor are less dependent on other parameters when multiple PPs per sample are utilized, with signal enhancement values of up to 111 and 339 for nortriptyline and haloperidol, respectively. All the findings and strategies presented herein constitute significant contributions that can facilitate future research in method development, particularly in the utilization of PP‐HF‐EE and similar EE approaches.

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Liquid-phase microextraction

Kokosa

2024

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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Environmental Applications of Electromembrane Extraction: A Review

Cited by 9 publications

References 103 publications

Confined Gel-Electromembrane Extraction of Oligonucleotides

Confined Gel-Electromembrane Extraction of Oligonucleotides

Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance

Liquid-phase microextraction

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