Environmental applications of immobilized microbial cells: A review

Cassidy, Mike; Lee, H; Trevors, J. T.

doi:10.1007/bf01570068

Cited by 483 publications

(294 citation statements)

References 198 publications

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“…Formulation of inoculant carrier is an industrial art of converting a promising laboratory proven bacteria to a commercial field product (Bashan, 1998). Immobilization of microbial cells into polymer matrix has proved to be advantageous over direct soil inoculation (Cassidy et al, 1996). The main goals of encapsulation of PGPB is to protect them from harsh soil environment, reduce microbial competition and release them gradually to facilitate colonization of plant roots (Bashan et al, 2002;Vassilev et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Young¹,

Rekha²,

Lai³

et al. 2006

Biotech & Bioengineering

154

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The key to achieving successful, reproducible results following the introduction of beneficial microbes into soil relies on the survival rate of the inoculated bacteria in a heterogeneous soil environment and hence an improved encapsulation method was developed. Owing to the constraints associated with the inoculum formulation, in this study, encapsulation of a plant growth promoting bacteria (PGPB) isolate Bacillus subtilis CCpg104 was attempted with alginate by enriching the bead microenvironment with humic acid. High viability of the encapsulated bacteria was observed with minimum cell loss upon storage for 5 months. Steady and constant cell release from the bead was observed for 1 week at different pH. Encapsulated cells remained active as evidenced by their ability to solubilize calcium phosphate in vitro. Successful plant growth promotion of lettuce by the encapsulated bacteria under gnotobiotic and sterile environment was also achieved. Feasibility of this improved encapsulation technique is mainly due to the dual benefits of humic acid to microbe and plant and its chemical properties allowing an easy mixing with alginate without interfering in the formation of the alginate gel beads by cross-linking with Ca 2þ ions. Thus, the encapsulation method described in this study can be effectively used to protect the PGPB inoculum from adverse conditions of the soil for their successful establishment in the rhizosphere. ß

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Section: Introductionmentioning

confidence: 99%

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Young¹,

Rekha²,

Lai³

et al. 2006

Biotech & Bioengineering

154

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“…However, cell-aggregates (\ 10 4 lm 3 ) showed already an amplified size from day 3 onwards (Xu et al 2013). These are necrotic and apoptotic niches located in the center of tumors (Cassidy et al 1996). We are confident that these aggregates are essential for the development of in vitro tumor tissue and further investigations.…”

Section: Discussionmentioning

confidence: 72%

“…A suitable natural and biocompatible polysaccharide for immobilization of cells in a 3D construct is alginate. Popular 3D in vitro models are alginate beads, which are mainly used for environmental (Cassidy et al 1996) and basic research like drug development (Rimann and Graf-Hausner 2012), regenerative medicine (Jonitz et al 2011b;Fischbach et al 2009;Moshaverinia et al 2013a) including tissue engineering (Chen et al 2010) and investigation of cancer (Fischbach et al 2009). The alginate beads can also be used to encapsulate chondrocytes.…”

Section: Introductionmentioning

confidence: 99%

3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

et al. 2015

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Cancer diseases are a common problem of the population caused by age and increased harmful environmental influences. Herein, new therapeutic strategies and compound screenings are necessary. The regular 2D cultivation has to be replaced by three dimensional cell culturing (3D) for better simulation of in vivo conditions. The 3D cultivation with alginate matrix is an appropriate method for encapsulate cells to form cancer constructs. The automated manufacturing of alginate beads might be an ultimate method for large-scaled manufacturing constructs similar to cancer tissue. The aim of this study was the integration of full automated systems for the production, cultivation and screening of 3D cell cultures. We compared the automated methods with the regular manual processes. Furthermore, we investigated the influence of antibiotics on these 3D cell culture systems. The alginate beads were formed by automated and manual procedures. The automated steps were processes by the Biomek Ò Cell Workstation (celisca, Rostock, Germany). The proliferation and toxicity were manually and automatically evaluated at day 14 and 35 of cultivation. The results visualized an accumulation and expansion of cell aggregates over the period of incubation. However, the proliferation and toxicity were faintly and partly significantly decreased on day 35 compared to day 14. The comparison of the manual and automated methods displayed similar results. We conclude that the manual production process could be replaced by the automation. Using automation, 3D cell cultures can be produced in industrial scale and improve the drug development and screening to treat serious illnesses like cancer.

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“…Имеются данные о значительных изменениях формы и увеличении размеров иммобилизованных клеток [39], трихомов и колоний [2].…”

Section: влияние иммобилизации на клетки микроводорослейunclassified

“…Влияние иммобилизации на фотосинтетиче-скую активность клеток МВ неоднозначно [39][40][41] и зависит от изменения эффективной освещенности клеток. В случае недостаточного, либо избыточного освещения иммобилизованных клеток скорость фотосинтеза падает, но носитель может и защищать клетки от фотоповреждения, рассеивая избыточный свет.…”

Section: влияние иммобилизации на клетки микроводорослейunclassified

Immobilized microalgae in biotechnology

Vasilieva

Лобакова

Lukyanov

et al. 2016

Moscow Univ. Biol.Sci. Bull.

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Рассматривается иммобилизация клеток оксигенных фототрофных микроорганиз-мов -цианобактерий и эукариотических микроводорослей -в природе и в искусствен-ных системах. В обзоре подчеркивается, что существование клеток микроорганизмов в прикрепленном состоянии, например, в составе биопленок, является широко распро-страненной в природе стратегией, обеспечивающей выживание клеток. Таким образом, искусственно иммобилизованные клетки оксигенных фототрофных микроорганизмов можно рассматривать как особую группу биомиметических материалов. Особое внимание уделено изучению влияния различных способов иммобилизации на физиологическое состояние клеток цианобактерий и микроводорослей, их устойчивость к стрессовым воз-действиям, а также продуктивность культур, находящихся в иммобилизованном состоя-нии. В обзоре проводится анализ преимуществ и недостатков современных методов им-мобилизации и используемых в настоящее время носителей. Освещаются возможности применения иммобилизованных культур оксигенных фототрофных микроорганизмов в различных областях биотехнологии, таких как получение биомассы и ценных метаболи-тов, сбор биомассы, очистка водных акваторий и сточных вод от тяжелых металлов, из-бытка биогенных элементов и органических загрязнителей.

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Environmental applications of immobilized microbial cells: A review

Cited by 483 publications

References 198 publications

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

Encapsulation of plant growth‐promoting bacteria in alginate beads enriched with humic acid

3 dimensional cell cultures: a comparison between manually and automatically produced alginate beads

Immobilized microalgae in biotechnology

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