Environmental assessment reveals the presence of MLB-1 human astrovirus in Uruguay

Lizasoain, Andrés; Tort, Luis Fernando López; González-García, María J.; Gómez, Mariela Martínez; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira; Cristina, Juan; Colina, Rodney; Victoria, Matías

doi:10.1111/jam.12856

Cited by 27 publications

(16 citation statements)

References 36 publications

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“…Diversity of human AstV strains in water samples was studied by Sanger sequencing in previous studies 17 – 19 , 21 – 23 , except for one study applying next-generation sequencing 37 . Especially, diversities of MLB- and VA-AstVs in water samples were studied by only one 17 and two studies 17 , 18 , respectively. Therefore, only a limited number of genotypes/strains have been identified from water samples.…”

Section: Discussionmentioning

confidence: 99%

“…Two previous studies have investigated the presence of MLB-AstVs in water samples 17 , 18 . Each study identified one genotype, i.e., one study identified type 2 17 and the other identified type 1 18 . In the present study, multiple types of MLB-AstVs were identified.…”

Section: Discussionmentioning

confidence: 99%

“…During these two decades since the first identification of human AstVs in the water environment in Spain 16 , only a few studies have focused on human AstVs in water 1 . Particularly, presence of MLB- and VA-AstVs in environmental water samples has been reported by only two studies conducted in Japan and Uruguay 17 , 18 . Both studies revealed that genetically diverse human AstV strains are present in the environment 17 , 18 .…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, presence of MLB- and VA-AstVs in environmental water samples has been reported by only two studies conducted in Japan and Uruguay 17 , 18 . Both studies revealed that genetically diverse human AstV strains are present in the environment 17 , 18 . This motivated us to investigate the presence of human AstVs in other regions.…”

Section: Introductionmentioning

confidence: 99%

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Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters

Hata

Kitajima

Haramoto

et al. 2018

Sci Rep

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Human astroviruses are associated with gastroenteritis and known to contaminate water environments. Three different genetic clades of astroviruses are known to infect humans and each clade consists of diverse strains. This study aimed to determine the occurrence and genetic diversity of astrovirus strains in water samples in different geographical locations, i.e., influent and effluent wastewater samples (n = 24 each) in Arizona, U.S., and groundwater (n = 37) and river water (n = 14) samples collected in the Kathmandu Valley, Nepal, using next-generation amplicon sequencing. Astrovirus strains including rare types (types 6 and 7 classical human astroviruses), emerging type (type 5 VA-astroviruses), and putative recombinants were identified. Feline astrovirus strains were collaterally identified and recombination between human and feline astroviruses was suggested. Classical- and VA-astroviruses seemed to be prevalent during cooler months, while MLB-astroviruses were identified only during warmer months. This study demonstrated the effectiveness of next-generation amplicon sequencing for identification and characterization of genetically diverse astrovirus strains in environmental water.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters

Hata

Kitajima

Haramoto

et al. 2018

Sci Rep

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“…Recently, the emergence of MLB and VA HAstV genotypes have been also observed in sewage samples in Japan and Uruguay (Hata et al, 2015;Lizasoain et al, 2015). Sewage surveillance conducted in sewage samples from Uruguay also detected canine astrovirus clustering with strains detected previously in Italy (2008) and Brazil (2012) (Lizasoain et al, 2015b).…”

Section: Nr -Not Reportedmentioning

confidence: 72%

Rotavirus and Astrovirus

Silva¹,

Victoria²,

Miagostovich³

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Epidemiology and genetic diversity of classic human astrovirus among hospitalized children with acute gastroenteritis in Uruguay

López

Lizasoain

Victoria

et al. 2017

Journal of Medical Virology

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Classic Human Astrovirus (Classic HAstV) are one of the most important causes of pediatric acute gastroenteritis (AGE), after rotaviruses and arguably caliciviruses. The aim of this study was to determine the molecular epidemiology of Classic HAstV from 175 clinical samples, being 153 stools and 22 vomits, collected from pediatric hospitalized patients with AGE in Salto city, Uruguay, from January 2011 to December 2012. Classic HAstV were detected and genotyped by using a qualitative Retro Transcription-Polimerase Chain Reaction (RT-PCR) directed to the Open Reading Frame-2 (ORF2) region C. Amplicons were sequenced and phylogenetic analyses were carried out in order to determine genotypes and lineages. Classic HAstV were detected in 18 out of 175 analyzed samples (10.3%) and 14 of them (78.0%) were successfully sequenced being 6 (42.8%) classified as HAstV-1 (1a lineage), 4 (28.6%) as HAstV-2 (2c lineage), and 4 (28.6%) as HAstV-3 (3c lineage). A higher detection of Classic HAstV infections was observed in autumn for both years of surveillance, and the majority of the positive cases were observed in 2011. The group of children between 2 and 5 years old presented the higher percentage of infections. To our knowledge, the present study represents the first report of astrovirus from acute gastroenteritis cases in Uruguay, evidencing its role as a relevant etiologic agent in severe cases of this disease.

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Environmental assessment reveals the presence of MLB-1 human astrovirus in Uruguay

Abstract: This study highlights the benefits of an environmental surveillance to study emerging enteric viruses circulating in human societies.

Cited by 27 publications

References 36 publications

Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters

Next-generation amplicon sequencing identifies genetically diverse human astroviruses, including recombinant strains, in environmental waters

Rotavirus and Astrovirus

Epidemiology and genetic diversity of classic human astrovirus among hospitalized children with acute gastroenteritis in Uruguay

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