Environmental bacteria produce abundant and diverse antibiofilm compounds

Farmer, J.T.; Shimkevitch, Anton V.; Reilly, P.; Mlynek, Kevin D.; Jensen, Kai; Callahan, Mary Theresa; Bushaw-Newton, Karen L.; Kaplan, Jeffrey B.

doi:10.1111/jam.12639

Cited by 12 publications

(6 citation statements)

References 42 publications

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“…In the current study, we isolated DNase-producing soil bacteria belonging to Bacillus, Chryseobacterium, Fictibacillus, Flavobacterium, Microbacterium, Nubsella, Pseudomonas, Psychrobacillus, Rheinheimera, Serratia and Stenotrophomonas genera. This observation is in agreement with the findings of Farmer et al (2014) who isolated soil DPB belonging to the Bacillus, Pseudomonas, Serratia and Strenotrophomonas genera; Balestrazzi et al (2007) who isolated DPB belonging to the genera Bacillus, Microbacterium, Pseudomonas and Stenotrophomonas and Aparna and Sarada (2012) who isolated several DPB belonging to Serratia genera. Although we only identified six Bacillus species (B. cereus, B. muralis, B. mycoides, B. pumilus, B. simplex and B. thuringiensis) that produce exDNase, several other Bacillus species have been reported to exhibit extracellular nuclease activity including B. subtilis (Akrigg and Mandelstam 1978;Moreno et al 2012); B. licheniformis (Nijland et al 2010); B. fusiformis, B. megaterium, B. sphericus, B. brevis (Balestrazzi et al 2007); and B. seohaeanensis, B. stratosphericus, B. oceanisediminis, B. mojavensis (Moreno et al 2012).…”

Section: Discussionsupporting

confidence: 91%

The effect of crop species on DNase-producing bacteria in two soils

Kamino

Gulden

2021

Ann Microbiol

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Purpose Extracellular deoxyribonucleases (exDNases) from microbial origin contribute substantially to the restriction of extracellular DNA (exDNA) in the soil. Hence, it is imperative to understand the diversity of bacterial species capable of performing this important soil function and how crop species influence their dynamics in the soil. The present study investigates the occurrence of DNase-producing bacteria (DPB) in leachate samples obtained from soils in which the crop species of alfalfa (Medicago sativa L.), canola (Brassica napus L.), soybean (Glycine max [L.] Merr.) and wheat (Triticum aestivum L.) were raised in a growth room. Methods Selective media containing methyl green indicator was used to screen for DPB from leachate samples, whereas the 16S rRNA sequence analysis was employed to identify the isolates. Results The proportion of culturable DPB ranged between 5.72 and 40.01%; however, we did observe specific crop effects that shifted throughout the growing period. In general, higher proportions of exDNase producers were observed when the soils had lower nutrient levels. On using the 16S rRNA to classify the DPB isolates, most isolates were found to be members of the Bacillus genera, while other groups included Chryseobacterium, Fictibacillus, Flavobacterium, Microbacterium, Nubsella, Pseudomonas, Psychrobacillus, Rheinheimera, Serratia and Stenotrophomonas. Five candidate exDNase/nuclease-encoding proteins were also identified from Bacillus mycoides genomes using online databases. Conclusion Results from this study showed that crop species, growth stage and soil properties were important factors shaping the populations of DPB in leachate samples; however, soil properties seemed to have a greater influence on the trends observed on these bacterial populations. It may be possible to target soil indigenous bacteria that produce exDNases through management to decrease potential unintended effects of transgenes originating from genetically modified organisms (GMOs) or other introduced nucleic acid sequences in the environment.

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Section: Discussionsupporting

confidence: 91%

The effect of crop species on DNase-producing bacteria in two soils

Kamino

Gulden

2021

Ann Microbiol

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“…The system is closed; therefore, there is no flow in or out of the reactor during experimentation. Consequently, the environment in the experimental model changes, for example, with regard to the availability of nutrients and molecules [ 54 ]. The microtiter plate-based system is used to perform different tests at the same time, which may be ideal for rapid screening of methods for biofilm disinfection and removal [ 55 ].…”

Section: Methods Of Studying Biofilms In Endodontics—concepts Frommentioning

confidence: 99%

Biofilms in Endodontics—Current Status and Future Directions

Neelakantan

Romero

Vera

et al. 2017

IJMS

183

136

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Microbiota are found in highly organized and complex entities, known as biofilms, the characteristics of which are fundamentally different from microbes in planktonic suspensions. Root canal infections are biofilm mediated. The complexity and variability of the root canal system, together with the multi-species nature of biofilms, make disinfection of this system extremely challenging. Microbial persistence appears to be the most important factor for failure of root canal treatment and this could further have an impact on pain and quality of life. Biofilm removal is accomplished by a chemo-mechanical process, using specific instruments and disinfecting chemicals in the form of irrigants and/or intracanal medicaments. Endodontic research has focused on the characterization of root canal biofilms and the clinical methods to disrupt the biofilms in addition to achieving microbial killing. In this narrative review, we discuss the role of microbial biofilms in endodontics and review the literature on the role of root canal disinfectants and disinfectant-activating methods on biofilm removal.

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“…Moreover, Pseudomonas fluorescens was shown to produce several compounds of different classes exhibiting strong antimicrobial activities, such as glycolipids [52], cyclic lipopeptides [53], rhamnolipids [54], phenazines [55] and polyketides [56]. In another study, Farmer et al [57], isolated various Pseudomonas sp. strains from soil that inhibited the biofilm formation of S.…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium

et al. 2020

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In the marine environment, most solid surfaces are covered by microbial biofilms, mainly composed of bacteria and diatoms. The negative effects of biofilms on materials and equipment are numerous and pose a major problem for industry and human activities. Since marine microorganisms are an important source of bioactive metabolites, it is possible that they synthesize natural ecofriendly molecules that inhibit the adhesion of organisms. In this work, the antibiofilm potential of marine bacteria was investigated using Flavobacterium sp. II2003 as a target. This strain is potentially a pioneer strain of bacteria that was previously selected from marine biofilms for its strong biofilm-forming ability. The culture supernatants of 86 marine heterotrophic bacteria were tested for their ability to inhibit Flavobacterium sp. II2003 biofilm formation and the Pseudomonas sp. IV2006 strain was identified as producing a strong antibiofilm activity. The Pseudomonas sp. IV2006 culture supernatant (SN IV2006) inhibited Flavobacterium sp. II2003 adhesion without killing the bacteria or inhibiting its growth. Moreover, SN IV2006 had no effect on the Flavobacterium sp. II2003 cell surface hydrophilic/hydrophobic and general Lewis acid-base characteristics, but modified the surface properties of glass, making it on the whole more hydrophilic and more alkaline and significantly reducing bacterial cell adhesion. The glass-coating molecules produced by Pseudomonas sp. IV2006 were found to probably be polysaccharides, whereas the antibiofilm molecules contained in SN IV2006 and acting during the 2 h adhesion step on glass and polystyrene surfaces would be proteinaceous. Finally, SN IV2006 exhibited a broad spectrum of antibiofilm activity on other marine bacteria such as Flavobacterium species that are pathogenic for fish, and human pathogens in both the medical environment, such as Staphylococcus aureus and Pseudomonas aeruginosa, and in the food industry, such as Yersinia enterocolitica. Thus, a wide range of applications could be envisaged for the SN IV2006 compounds, both in aquaculture and human health.

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Environmental bacteria produce abundant and diverse antibiofilm compounds

Abstract: Screening cell-free extracts is a useful method for identifying secreted compounds that regulate biofilm formation. Such compounds may represent a novel source of antibiofilm agents for technological development.

Cited by 12 publications

References 42 publications

The effect of crop species on DNase-producing bacteria in two soils

The effect of crop species on DNase-producing bacteria in two soils

Biofilms in Endodontics—Current Status and Future Directions

Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium

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