Environmental biofilm communities associated with early‐stage common dentex (Dentex dentex) culture

Abstract

Aims: To describe the biofilm microbiota associated with various feeding phases during larval common dentex (Dentex dentex) culture. Methods and Results: A targeted metagenomic (metagenetic) study was performed by means of 16S rRNA gene-based PCR and NextGen pyrosequencing. The resulting dataset was scrutinized with microbial community analysis software (R packages) using R/Rstudio. While median observed and estimated alpha-diversities were 171 AE 38 and 207 AE 27 taxa, respectively, 72Á1-85Á8% of individual biofilm communities comprised only 27-46 taxa. Members of the genus Methylobacterium and family Rhodobacteraceae dominated biofilms formed during all feeding phases while genera Nannochloropsis and Tetraselmis microalgae were major constituents of biofilms during rotifer live feeding. Both potential fish pathogenic genera, for example, Vibrio and putatively probiotic taxa, for example, Phaeobacter gallaeciensis were identified. Conclusions: Relatively stable biofilm communities were identified during each feeding phase but varied significantly between feeding phases, most likely in response to the introduction of live feed/microalgae-associated bacteria into rearing tanks. Significance and Impact of the Study: The structure of the bacterial communities identified represents a 'template' for successful larval dentex culture and provides a foundation for future investigations into failed production cycles.

Pathogenic and Opportunistic Vibrio spp. Associated with Vibriosis Incidences in the Greek Aquaculture: The Role of Vibrio harveyi as the Principal Cause of Vibriosis

“…Many virulent strains were found in a gilthead seabream hatchery in Malta, causing morbidity and mortalities [ 35 ]. The species, along with other opportunistic pathogens, becomes part of the microbiota when fish are introduced to live feeds [ 36 , 37 ] or through the rearing tanks and water inlets [ 34 ]. In our study, very few V. harveyi strains were isolated from the hatchery environment (tanks, live feeds) and only in two cases was this species related to disease outbreak.…”

Morphological and Molecular Identification of Biofilm Forming Fungi from Fish Farms and Fish Benches in Aydın Province

“…In addition, most pathogenic fungi are free-living and pose no threat. However, biofilms, bacterial or fungal, provide a suitable and protective environment for potential pathogens (Turgay et al, 2019). Most of the aquatic fungi get their nutrients by decomposing organic matter (Chouldhury et al, 2013).…”

Microplastics in the environment and the analysis: fulfil knowledge gap of research size covering, methodology and analytical technologies

“…Once MPs enter the environment, they will undergo weathering governed by environmental conditions such as oxygen levels, light, temperature, and biofilm coverage (Mei et al, 2020;Turgay et al, 2019;Wang et al, 2020aWang et al, , 2020b. In general, marine water has a lower temperature than land and will also partly protect the MPs against sunlight.…”