Environmental Biology of the Marine <i>Roseobacter</i> Lineage

Wagner‐Döbler, Irene; Biebl, Hanno

doi:10.1146/annurev.micro.60.080805.142115

Cited by 435 publications

(379 citation statements)

References 112 publications

(53 reference statements)

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“…5a, b), which is widely distributed and observed frequently in marine environments (Wagner-Döbler and Biebl 2006). Hence, it is not surprising that this group was already identified to be associated with copepods in recent studies (Grossart et al 2009;Tang et al 2009a, b;Møller 2007Møller et al 2007Peter and Sommaruga 2008).…”

Section: Discussionmentioning

confidence: 95%

“…As ecological generalists, Roseobacter harbour large gene inventories and a remarkable suite of mechanisms by which to obtain carbon and energy (Newton et al 2010). Members of the Roseobacter lineage play an important role for the global carbon and sulphur cycle, for example, and are able to produce dimethylsulfide (DMS) (Wagner-Döbler and Biebl 2006). Dimethylsulfoniumpropionate (DMSP)-consuming bacteria were already found to be associated with the marine calanoid copepod Acartia tonsa by Tang et al (2001).…”

Section: Discussionmentioning

confidence: 99%

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The microbiome of North Sea copepods

et al. 2013

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Copepods can be associated with different kinds and different numbers of bacteria. This was already shown in the past with culture-dependent microbial methods or microscopy and more recently by using molecular tools. In our present study, we investigated the bacterial community of four frequently occurring copepod species, Acartia sp., Temora longicornis, Centropages sp. and Calanus helgolandicus from Helgoland Roads (North Sea) over a period of 2 years using DGGE (denaturing gradient gel electrophoresis) and subsequent sequencing of 16S-rDNA fragments. To complement the PCR-DGGE analyses, clone libraries of copepod samples from June 2007 to 208 were generated. Based on the DGGE banding patterns of the two years survey, we found no significant differences between the communities of distinct copepod species, nor did we find any seasonality. Overall, we identified 67 phylotypes ([97 % similarity) falling into the bacterial phyla of Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The most abundant phylotypes were affiliated to the Alphaproteobacteria. In comparison with PCR-DGGE and clone libraries, phylotypes of the Gammaproteobacteria dominated the clone libraries, whereas Alphaproteobacteria were most abundant in the PCR-DGGE analyses.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

The microbiome of North Sea copepods

et al. 2013

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“…A high proportion of bacterioneuston coxL sequences detected were similar to coxL from S. pomeroyi, a known marine CO oxidizer (Moran et al, 2004). S. pomeroyi is part of the Roseobacter lineage, a physiologically diverse group of Alphaproteobacteria that forms a numerically significant component of marine microbial communities (Buchan et al, 2005;Wagner-Dö bler and Biebl, 2006). The Roseobacter group has been shown to be responsible for a large amount of CO oxidation in coastal marine waters (Tolli et al, 2006).…”

Section: Bacterial Functional Gene Diversitymentioning

confidence: 99%

Phylogenetic and functional gene analysis of the bacterial and archaeal communities associated with the surface microlayer of an estuary

et al. 2008

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The surface microlayer (SML) is the thin biogenic film found at the surface of a water body. The SML is poorly understood but has been shown to be important in biogeochemical cycling and sea-air gas exchange. We sampled the SML of the Blyth estuary at two sites (salinities 21 and 31 psu) using 47 mm polycarbonate membranes. DNA was extracted from the SML and corresponding subsurface water (0.4 m depth) and microbial (bacteria and archaea) community analysis was performed using denaturing gradient gel electrophoresis of 16S rRNA gene PCR amplicons. The diversity of bacterial functional genes that encode enzyme subunits for methane monooxygenase (pmoA and mmoX) and carbon monoxide dehydrogenase (coxL) was assessed using PCR, clone library construction and restriction fragment length polymorphism (RFLP) analysis. Methanotroph genes were present only in low copy numbers and pmoA was detected only in subsurface samples. Diversity of mmoX genes was low and most of the clone sequences detected were similar to those of mmoX from Methylomonas spp. Interestingly, some sequences detected in the SML were different from those detected in the subsurface. RFLP analysis of coxL clone libraries indicated a high diversity of carbon monoxide (CO)-utilizing bacteria in the estuary. The habitats of the closely related coxL sequences suggest that CO-utilizing bacteria in the estuary are recruited from both marine and freshwater/terrestrial inputs. In contrast, methanotroph recruitment appears to occur solely from freshwater input into the estuary.

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“…A bacterial taxon which has received considerable effort in terms of genome sequencing is the Marine Roseobacter Clade (MRC) Newton et al, 2010). The MRC is a phylogenetically coherent group in the order Rhodobacterales and is one of the most numerically abundant marine lineages, especially in coastal and polar waters, accounting for up to 25% of the bacterioplankton (Giovannoni and Rappé, 2000;Wagner-Dö bler and Biebl, 2006;Brinkhoff et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Correlating carbon monoxide oxidation with cox genes in the abundant Marine Roseobacter Clade

Cunliffe

2010

The ISME Journal

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The Marine Roseobacter Clade (MRC) is a numerically and biogeochemically significant component of the bacterioplankton. Annotation of multiple MRC genomes has revealed that an abundance of carbon monoxide dehydrogenase (CODH) cox genes are present, subsequently implying a role for the MRC in marine CO cycling. The cox genes fall into two distinct forms based on sequence analysis of the coxL gene; forms I and II. The two forms are unevenly distributed across the MRC genomes. Most (18/29) of the MRC genomes contain only the putative form II coxL gene. Only 10 of the 29 MRC genomes analysed have both the putative form II and the definitive form I coxL. None have only the form I coxL. Genes previously shown to be required for post-translational maturation of the form I CODH enzyme are absent from the MRC genomes containing only form II. Subsequent analyses of a subset of nine MRC strains revealed that only MRC strains with both coxL forms are able to oxidise CO.

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Environmental Biology of the Marine Roseobacter Lineage

Cited by 435 publications

References 112 publications

The microbiome of North Sea copepods

The microbiome of North Sea copepods

Phylogenetic and functional gene analysis of the bacterial and archaeal communities associated with the surface microlayer of an estuary

Correlating carbon monoxide oxidation with cox genes in the abundant Marine Roseobacter Clade

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