Environmental chemicals with known endocrine potential affect yolk protein content in the aquatic insect Chironomus riparius

Hahn, Torsten; Schenk, Kamilla; Schulz, Ralf

doi:10.1016/s0269-7491(02)00189-6

Cited by 44 publications

(17 citation statements)

References 12 publications

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“…Chironomus riparius have also been observed in naturally occurring and anthropogenically induced salinated water (Colbo, 1996;Williams and Williams, 1998). Endocrine disruptors affect midge larvae by altering the accumulation of yolk protein in eggs, inducing expression of Hsp70, activating the enzymes catalase and glutathione-S-transferase, and reducing the rate of successful adult emergence (Lee and Choi, 2007;Morales et al, 2011;Hahn et al, 2002). Despite their importance, very little is known about the physiological mechanisms that underlie the observed responses to xenobiotics, heavy metals, endocrine disruptors and salinity in midge larvae.…”

mentioning

confidence: 99%

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Robertson

Chasiotis

Galperin

et al. 2014

Journal of Experimental Biology

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Evidence for the presence of allatostatin (AST) A-like neuropeptides in the larval midge Chironomus riparius is reported. Immunohistochemical studies on the nervous system and gut revealed the presence of AST A-like immunoreactive (AST-IR) cells and processes. The nerve cord contained AST-IR processes that originated from cells in the brain and travelled the length of nerve cord to the terminal ganglion. Within each ganglion, these processes gave rise to varicosities, suggesting that they formed synapses with neurons in the ganglia. Endocrine cells containing AST-IR were present in three regions of the midgut: near the attachment of the Malpighian tubules, between the anterior and posterior midgut, and in the vicinity of the gastric caecae. The terminal ganglion also contained four AST-IR cells that gave rise to axons that projected onto the hindgut and posterior midgut. Application of a cockroach AST to the semi-isolated hindgut of larval C. riparius led to dosedependent inhibition of muscle contractions with an EC 50 of 10 nmol l −1 and a decrease in rectal K + reabsorption resulting from reduced rectal Na + /K + -ATPase and vacuolar type H + -ATPase activities. The results suggest the presence of endogenous AST-like neuropeptides in larval C. riparius, where these factors play a role in the function of the gut. Furthermore, regulation of ion reabsorption by ASTs at the rectum could serve as an ideal mechanism of ion regulation in the face of abrupt and acute elevated salt levels. KEY WORDS: Na INTRODUCTIONJuvenile hormones are synthesized by the corpora allata and control the juvenile characteristics of insects during growth and development. In adult insect females, juvenile hormones can also regulate reproductive activities such as the maturation of oocytes. Synthesis of juvenile hormones is inhibited by allatostatins (ASTs), members of a large and diverse family of peptides that are structurally characterized into three distinct groups (Gäde and Goldsworthy, 2003).The C-type allatostatins with an N-terminal pGlu residue and a free C terminus have been discovered in the moth Manduca sexta, the fly Drosophila melanogaster and the mosquito Aedes aegypti (Kramer et al., 1991;Williamson et al., 2001;Li et al., 2006). The B-type allatostatins all have a common Trp-(X6)-Trp-amide C terminus and were first discovered in the cricket (Wang et al., 2004). The A-type allatostatins (ASTs) were the first to be discovered in the cockroach and share a Phe-Gly-Leu-amide C terminus (Donly et al., 1993;Siju et al., 2014). RESEARCH ARTICLEDepartmentAll allatostatins inhibit juvenile hormone synthesis in at least one group or species of insect; however, many do not in other insects, and instead have known inhibitory activity on contractile properties of muscle (Gäde and Goldsworthy, 2003). In particular, the ASTs have been shown to inhibit contractions of visceral muscle including the foregut and hindgut (Lange et al., 1993;Sarkar et al., 2003;Predel et al., 2001;Matthews et al., 2008), and have been localized to end...

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mentioning

confidence: 99%

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Robertson

Chasiotis

Galperin

et al. 2014

Journal of Experimental Biology

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“…Yolk concentrations in males decreased by 20 to 25% after exposure to BPA in all concentrations, while females were only affected in the highest BPA treatment, where yolk immunoreactivity was reduced by about 10% compared to the control [36].…”

Section: Bisphenol-amentioning

confidence: 77%

“…Hahn et al [36] detected, in a semi-static test system using 1.0, 100 and 3000 μg/L of BPA, an alteration of vitellogenin/vitellin production in males and female. Yolk concentrations in males decreased by 20 to 25% after exposure to BPA in all concentrations, while females were only affected in the highest BPA treatment, where yolk immunoreactivity was reduced by about 10% compared to the control [36].…”

Section: Bisphenol-amentioning

confidence: 99%

“…The process of vitellogenesis is under hormonal control and the hormones involved are ecdysteroids and juvenile hormones, which in the adult insect, do not trigger molting processes but play a new role in gonadal maturation [49]. Hahn et al [36] determined in a semistatic test system using 4NP (1.9 μg/L-2.0 mg/l) an alteration of vitellogenin/ vitellin production in males. The results showed a significant reduction in males' yolk immunoreactivity at 1.9 and 30 μg/L and an increase at 2.0 mg/L.…”

Section: Butyl Benzyl Phthalatementioning

confidence: 99%

“…This sexrelated difference probably resulted from the endocrine activity of Tebufenozide. One year later, Hahn et al [36], tested vitellogenesis as a marker for possible effects of endocrine-disrupting agents using concentrations that are environmentally relevant. Tebufenozide did not affect the yolk protein content after a semistatic exposure of 10 μg/L but it reduced the yolk protein content of males at 80 μg/L.…”

Section: Tebufenozidementioning

confidence: 99%

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Endocrine Disrupting Compounds in Lotic Ecosystems: A Review on Its Occurrence, Sources and Effects on Chironomus riparius

Pinheiro¹,

Pereira²,

Vieira³

2017

Environ Pollut Climate Change

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Transcriptional responses of xenobiotic metabolizing enzymes, HSP70 and Na⁺/K⁺‐ATPase in the liver of rabbitfish (Siganus oramin) intracoelomically injected with amnesic shellfish poisoning toxin

Wang

Liang

Huang

et al. 2008

Environmental Toxicology

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Amnesic shellfish poisoning toxin domoic acid (DA) is a marine neurotoxin that accumulates in fish and shellfish, and has been implicated to be involved in human and marine wildlife mortality. The transcriptional responses of cytochrome P-450 1A (CYP1A), glutathione S-transferase alpha (GSTA), glutathione S-transferase rho (GSTR), heat shock protein 70 (HSP70), and Na(+)/K(+)-ATPase alpha 1 (ATP1A1) in the liver of rabbitfish (Siganus oramin) intracoelomically injected with DA, were investigated. Experimental fish were administered with one injection of DA (2 microg/g wet weight) or PBS as control. After 24 h, fish were killed and hepatic RNA was isolated. Partial cDNA of rabbitfish CYP1A, GSTA, GSTR, HSP70, ATP1A1, and beta-actin were obtained by PCR using degenerate primers. Using beta-actin as an external control, the relative liver CYP1A, GSTA, GSTR, HSP70, and ATP1A1 mRNA abundance of rabbitfish were determined by semi-quantitative RT-PCR within the exponential phase. The ratio CYP1A/beta-actin mRNA (%) of exposure group was determined to be 148.92+/-12.69, whereas the ratio of control group was 82.3+/-8.35, indicating that CYP1A was induced significantly in rabbitfish following DA exposure (P<0.05). Although the expressions of GSTA, HSP70, and ATP1A1 tended to increase and GSTR tended to decrease, no significant changes were found (P>0.05). The induction of hepatic CYP1A in response to DA suggests a potential role for fish phase I xenobiotic metabolizing enzyme in DA metabolism.

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Environmental chemicals with known endocrine potential affect yolk protein content in the aquatic insect Chironomus riparius

Cited by 44 publications

References 12 publications

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Endocrine Disrupting Compounds in Lotic Ecosystems: A Review on Its Occurrence, Sources and Effects on Chironomus riparius

Transcriptional responses of xenobiotic metabolizing enzymes, HSP70 and Na⁺/K⁺‐ATPase in the liver of rabbitfish (Siganus oramin) intracoelomically injected with amnesic shellfish poisoning toxin

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Environmental chemicals with known endocrine potential affect yolk protein content in the aquatic insect Chironomus riparius

Cited by 44 publications

References 12 publications

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Allatostatin A-like immunoreactivity in the nervous system and gut of the larval midge, Chironomus riparius (Meigen): Modulation of hindgut motility, rectal K+ transport and implications for exposure to salinity

Endocrine Disrupting Compounds in Lotic Ecosystems: A Review on Its Occurrence, Sources and Effects on Chironomus riparius

Transcriptional responses of xenobiotic metabolizing enzymes, HSP70 and Na+/K+‐ATPase in the liver of rabbitfish (Siganus oramin) intracoelomically injected with amnesic shellfish poisoning toxin

Contact Info

Product

Resources

About

Transcriptional responses of xenobiotic metabolizing enzymes, HSP70 and Na⁺/K⁺‐ATPase in the liver of rabbitfish (Siganus oramin) intracoelomically injected with amnesic shellfish poisoning toxin