Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae

Bihanic, Florane Le; Sommard, Vivien; Perrine, de Lansalut; Pichon, Anaïk; Grasset, Julie; Berrada, Saadia; Budzinski, Hélène; Cousin, Xavier; Morin, Bénédicte; Cachot, Jérôme

doi:10.1016/j.ecoenv.2014.12.011

Cited by 28 publications

(14 citation statements)

References 39 publications

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“…In their studies, adult zebrafish which were developmentally exposed to individual PAHs or PAH mixtures, exhibited impaired locomotor activity, and increased anxiety compared to non-exposed fish. Additionally, similar results were reported using Japanese medaka embryos exposed to BAA (Le Bihanic et al, 2015), suggesting that PAHs are developmental neurotoxicants. The probability that some oxy-PAHs are also developmental neurotoxicants is high, however, a more comprehensive analysis is still needed.…”

Section: Resultssupporting

confidence: 84%

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

Elie

Choi

Nkrumah‐Elie

et al. 2015

Environmental Research

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Polycyclic aromatic hydrocarbons (PAHs) and their oxygenated derivatives are ubiquitously present in diesel exhaust, atmospheric particulate matter and soils sampled in urban areas. Therefore, inhalation or non-dietary ingestion of both PAHs and oxy-PAHs are major routes of exposure for people; especially young children living in these localities. While there has been extensive research on the parent PAHs, limited studies exist on the biological effects of oxy-PAHs which have been shown to be more soluble and more mobile in the environment. Additionally, investigations comparing the metabolic responses resulting from parent PAHs and oxy-PAHs exposures have not been reported. To address these current gaps, an untargeted metabolomics approach was conducted to examine the in vivo metabolomic profiles of developing zebrafish (Danio rerio) exposed to 4 µM of benz[a]anthracene (BAA) or benz[a]anthracene-7, 12-dione (BAQ). By integrating multivariate, univariate and pathway analyses, a total of 62 metabolites were significantly altered after 5 days of exposure. The marked perturbations revealed that both BAA and BAQ affect protein biosynthesis, mitochondrial function, neural development, vascular development and cardiac function. Our previous transcriptomic and genomic data were incorporated in this metabolomics study to provide a more comprehensive view of the relationship between PAH and oxy-PAH exposures on vertebrate development.

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Section: Resultssupporting

confidence: 84%

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

Elie

Choi

Nkrumah‐Elie

et al. 2015

Environmental Research

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“…2C) . A reduction in the swimming activity behavioral pattern was also recorded in Japanese medaka after exposure to benzo[a]anthracene (4-benzenic rings) (Bihanic et al 2015). The present study demonstrated a parental influence in the neurotoxic behavior of their offspring.…”

Section: Behavioral Disruptions In Offspring Larvae (F 1 Generation)supporting

confidence: 71%

“…In aquatic organisms, exposure to PAHs can thus lead to detrimental defects from a developmental, physiological and behavioral point of view (Bihanic et al 2015;Incardona et al 2011;Perrichon et al 2014). PAHs' impact on reproductive success is particularly studied due to its consequence on population recruitment.…”

Section: Introductionmentioning

confidence: 99%

Parental trophic exposure to three aromatic fractions of polycyclic aromatic hydrocarbons in the zebrafish: Consequences for the offspring

Perrichon

Akcha

Ménach

et al. 2015

Science of The Total Environment

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In recent decades, PAH emissions due to extensive anthropogenic activities have risen sharply causing considerable pollution of aquatic ecosystems. This pollution represents a threat for organisms, among them are fish. Consequently, prenatal stress can have important repercussions, and may impact survival and population recruitment. To investigate this point, eggs were collected from zebrafish exposed during 6 months by trophic route to three aromatic fractions from two different origins, pyrolytic (PY) and petrogenic (light (BAL) and heavy (HFO) fractions) sources. Chronic dietary exposure of the parents was performed at environmentally relevant concentrations (0.3×, 1× and 3×; 1× represents an environmental concentration measured in French estuary). In order to explore the consequences of parental exposure for the next first generation, toxic responses were studied in both embryos and larvae using a multiscale approach. Toxic effects were assessed by looking at hatching success, developmental abnormalities, photomotor response and heartbeat. The level of PAH metabolites and EROD activity in fish larvae were measured to assess exposure to PAHs. Egg production of parents was significantly reduced compared to the Control; hence little information was available for BAL and HFO offspring. The size of larvae from PY parents was found to increase despite a reduced yolk sac compared to Control larvae. Furthermore, a high level of behavioral stress was observed in larvae originating from parents exposed to three-fold the environmental concentration. The cardiac activity was reduced in a concentration-dependent manner for the PY exposure group. No effect was however observed on biotransformation markers (cyp1a, EROD), nor on the level of DNA damage for all PY, BAL and HFO offspring. The absence of significant differences in metabolite levels may indicate a potential early depuration of transferred compounds or no PAH-transmission. The disruptions observed at the individual level in the next generation could impact on the longer-term, surviving population.

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“…It is interesting to note that the embryotoxic and teratogenic effects observed in medaka embryos and larvae are highly correlated to organic pollutants and TOC content in sediments of the Bizerte lagoon (Table ). This result is in accordance with numerous studies reporting developmental and genotoxic effects of low or moderate concentrations of PAHs in Japanese medaka early life stages . In addition, among the low–molecular weight PAHs detected in the present study, the concentrations of fluorene and acenaphthene in the sediment exceeded the effects range low level .…”

Section: Discussionsupporting

confidence: 93%

Assessing the toxicity of sediments using the medaka embryo–larval assay and 2 other bioassays

Barhoumi

Clérandeau

Landi

et al. 2016

Enviro Toxic and Chemistry

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Sediments are sinks for aquatic pollutants, and analyzing toxicity in such complex matrices is still challenging. To evaluate the toxicity of bioavailable pollutants accumulated in sediments from the Bizerte lagoon (Tunisia), a novel assay, the medaka embryo-larval assay by sediment contact, was applied. Japanese medaka (Oryzias latipes) embryos were incubated in direct contact with sediment samples up to hatching. Lethal and sublethal adverse effects were recorded in embryos and larvae up to 20 d postfertilization. Results from medaka embryo-larval assay were compared with cytotoxicity (Microtox®), genotoxicity (SOS chromotest), and pollutant content of sediments. The results highlight differences in the contamination profile and toxicity pattern between the different studied sediments. A significant correlation was shown between medaka embryo-larval assay by sediment contact and SOS chromotest responses and concentrations of most organic pollutants studied. No correlation was shown between pollutant levels and Microtox. According to the number of sediment samples detected as toxic, medaka embryo-larval assay by sediment contact was more sensitive than Microtox, which in turn was more sensitive than the SOS chromotest; and medaka embryo-larval assay by sediment contact allowed sediment toxicity assessment of moderately polluted sediments without pollutant extraction and using an ecologically realistic exposure scenario. Although medaka embryo-larval assay by sediment contact should be tested on a larger sample set, the results show that it is sensitive and convenient enough to monitor the toxicity of natural sediments. Environ Toxicol Chem 2016;35:2270-2280. © 2016 SETAC.

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Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae

Cited by 28 publications

References 39 publications

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

Metabolomic analysis to define and compare the effects of PAHs and oxygenated PAHs in developing zebrafish

Parental trophic exposure to three aromatic fractions of polycyclic aromatic hydrocarbons in the zebrafish: Consequences for the offspring

Assessing the toxicity of sediments using the medaka embryo–larval assay and 2 other bioassays

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