Environmental Contamination Leading to False-Positive Polymerase Chain Reaction for Pertussis

Taranger, J; Trollfors, Birger; Lind, Lena; Zackrisson, Gunilla; Beling-Holmquist, Kerstin

doi:10.1097/00006454-199410000-00020

Cited by 41 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…False-positive results can occur if specimens for PCR are opened in the pertussis laboratory before going to the PCR laboratory (J. Cherry, unpublished data). In addition, they can occur as a result of contamination of the air in a room where DTP immunization is carried out (754).…”

Section: Specific Diagnosis Of B Pertussis Infectionsmentioning

confidence: 99%

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

2005

View full text Add to dashboard Cite

Bordetella respiratory infections are common in people (B. pertussis) and in animals (B. bronchiseptica). During the last two decades, much has been learned about the virulence determinants, pathogenesis, and immunity of Bordetella. Clinically, the full spectrum of disease due to B. pertussis infection is now understood, and infections in adolescents and adults are recognized as the reservoir for cyclic outbreaks of disease. DTaP vaccines, which are less reactogenic than DTP vaccines, are now in general use in many developed countries, and it is expected that the expansion of their use to adolescents and adults will have a significant impact on reducing pertussis and perhaps decrease the circulation of B. pertussis. Future studies should seek to determine the cause of the unique cough which is associated with Bordetella respiratory infections. It is also hoped that data gathered from molecular Bordetella research will lead to a new generation of DTaP vaccines which provide greater efficacy than is provided by today's vaccines

show abstract

Section: Specific Diagnosis Of B Pertussis Infectionsmentioning

confidence: 99%

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

2005

View full text Add to dashboard Cite

show abstract

“…However, PCR assays require a validated protocol, sophisticated technology, training, and rigorous quality assurance (QA). If handled improperly, reagents and assays can become contaminated with PCR amplicons or cellular DNA, leading to false-positive test results (34). Furthermore, PCR assays cannot differentiate between dead and viable organisms (11,16).…”

mentioning

confidence: 99%

Issues Associated with and Recommendations for Using PCR To Detect Outbreaks of Pertussis

et al. 2002

View full text Add to dashboard Cite

“…18 Hole-punch carryover contamination and environmental contamination of the laboratory work space and equipment have also been documented as potential sources of false-positive PCR results. 2,19 Results of this study highlight the importance of the microtome as a source of DNA contamination of paraffin-embedded tissues. Adherent fragments of paraffin or tissue from infected blocks can contaminate the next block to be processed.…”

Section: Vet Pathol 46:5 2009mentioning

confidence: 78%

Cross-contamination in the Molecular Detection of Bartonella from Paraffin-embedded Tissues

et al. 2009

View full text Add to dashboard Cite

Abstract. The genus Bartonella comprises a group of gram-negative, fastidious bacteria. Because of diagnostic limitations of culture and serologic testing, polymerase chain reaction (PCR) has become a powerful tool for the detection of Bartonella spp. in blood and tissue samples. However, because many wild and domestic animals harbor Bartonella spp., transfer of Bartonella DNA during sample collection or histologic processing could result in false-positive PCR test results. In this study, we describe evidence of Bartonella DNA dissemination and transfer in the necropsy room and during the subsequent processing of formalin-fixed paraffin-embedded tissues. Bartonella DNA was amplified from different areas of the necropsy room, from the liquid paraffin in the tissue processor, and from different parts of the microtome. Unless stringent procedures are established and followed to avoid cross-contamination, the molecular detection of Bartonella spp. from tissue samples obtained at necropsy or processed in a multispecies histopathology laboratory will not be reliable.

show abstract

Environmental Contamination Leading to False-Positive Polymerase Chain Reaction for Pertussis

Cited by 41 publications

References 0 publications

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

Issues Associated with and Recommendations for Using PCR To Detect Outbreaks of Pertussis

Cross-contamination in the Molecular Detection of Bartonella from Paraffin-embedded Tissues

Contact Info

Product

Resources

About