Environmental cues induce a long noncoding RNA–dependent remodeling of the nucleolus

Jacob, Mathieu D.; Audas, Timothy E.; Uniacke, James; Trinkle-Mulcahy, Laura; Lee, Stephen

doi:10.1091/mbc.e13-04-0223

Cited by 100 publications

(111 citation statements)

References 56 publications

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“…Nevertheless, our results, which demonstrate an ATPdependent decrease in nucleolar diffusion (i.e., an increase in molecular crowding), are consistent with previous results on the relative increase in nucleolar viscosity due to ATP depletion (42). As suggested in previous studies, our results support the conclusion that an ATP-dependent or transcriptional change in the microenvironment is a common phenomenon associated with active molecular processes in nucleolar assembly (18,42,43).…”

Section: Discussionsupporting

confidence: 93%

“…These results suggest that the nucleolar microenvironment is relatively charged compared with that of chromatin and that this microenvironment makes the mobility of charged molecules markedly slower, even though further investigation will be required to quantitating the mobility change of charged inert molecules in heterochromatin, mitotic chromosomes, and nucleoli, respectively (20,37). Recent study demonstrated that cellular stress induced formation of a subnucleolar structure called detention center consisted of long noncoding RNA, and mobility of the detention center-related nucleolar proteins, which have various phosphosites and pI values, were immobilized (43). It will be interesting to examine various types of electrostatic charge sequences in the probe molecule with the same molecular size.…”

Section: Discussionmentioning

confidence: 99%

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Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy

Park¹,

Han²,

Sako³

et al. 2014

FASEB j.

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Organization and functions of the nucleolus is maintained by mobilities and interactions of nucleolar factors. Because the nucleolus is a densely packed structure, molecular crowding effects determined by the molecular concentrations and mobilities in the nucleolus should also be important for regulating nucleolar organization and functions. However, such molecular property of nucleolar organization is not fully understood. To understand the biophysical property of nucleolar organization, the diffusional behaviors of inert green fluorescent protein (GFP) oligomers with or without nuclear localization signals (NLSs) were analyzed under various conditions by fluorescence correlation spectroscopy. Our result demonstrates that the mobility of GFPs inside the nucleolus and the nucleoplasm can be represented by single free diffusion under normal conditions, even though the mobility in the nucleolus is considerably slower than that in the chromatin region. Moreover, the free diffusion of GFPs is found to be significantly size-and NLS-dependent only in the nucleolus. Interestingly, the mobility in the nucleolus is highly sensitive to ATP depletion, as well as actinomycin D (ActD) treatment. In contrast, the ultra-structure of the nucleolus was not significantly changed by ATP depletion but was changed by ActD treatment. These results suggest that the nucleolus behaves similarly to an open aqueous-phase medium with an increased molecular crowding effect that depends on both energy and transcription.-Park, H., Han, S.-S., Sako, Y., Pack, C.-G. Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy. FASEB J. 29, 837-848 (2015). www.fasebj.org

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy

Park¹,

Han²,

Sako³

et al. 2014

FASEB j.

View full text Add to dashboard Cite

show abstract

“…Interestingly, all five nuclear bodies with arcRNA scaffolds form or increase in size or number upon cellular stresses or in specific developmental and disease conditions (Table 1). For instance, upon heat shock, much transcription and RNA processing is shut down, partly due to the sequestration of nucleic acid-binding proteins into nuclear bodies [34,41,42]. When such stresses are no longer present, arcRNAs can be quickly degraded and proteins trapped in stress-inducible nuclear bodies disperse into the nucleoplasm, rapidly allowing gene expression to return to normal.…”

Section: Figmentioning

confidence: 99%

Architectural RNAs (arcRNAs): A class of long noncoding RNAs that function as the scaffold of nuclear bodies

Chujo

Yamazaki

Hirose

2016

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

150

141

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“…Interestingly, the IGS lncRNA and its interacting proteins form a large subnucleolar structure named the detention center (DC), and the formation of the DC is dependent on the expression of IGS lncRNA (Jacob et al, 2013). A recent report has revealed that the maturation of pRNA, originated from the processing of an IGS lncRNA, is required for the establishment of heterochromatin at ribosomal RNA genes during ES cell differentiation (Savic et al, 2014).…”

Section: Prna and The Nucleolusmentioning

confidence: 99%

Long noncoding RNAs as Organizers of Nuclear Architecture

Cheng

Ming

Zhu

et al. 2016

Sci. China Life Sci.

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In the eukaryotic cell nucleus, chromatin and its associated macromolecules must be organized into a higher-ordered conformation to function normally. However, mechanisms underlying the organization and dynamics of the nucleus remain unclear. Long noncoding RNAs (lncRNAs), i.e., transcripts longer than 200 nucleotides with little or no protein-coding capacity, are increasingly recognized as important regulators in diverse biological processes. Recent studies have shown that some lncRNAs are involved in various aspects of genome organization, including the facilitation of chromosomal interactions and establishment of nuclear bodies, suggesting that lncRNAs act as general organizers of the nuclear architecture. Here, we discuss recent advances in this emerging and intriguing field. long noncoding RNAs, chromatin, nuclear architecture

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Environmental cues induce a long noncoding RNA–dependent remodeling of the nucleolus

Cited by 100 publications

References 56 publications

Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy

Dynamic and unique nucleolar microenvironment revealed by fluorescence correlation spectroscopy

Architectural RNAs (arcRNAs): A class of long noncoding RNAs that function as the scaffold of nuclear bodies

Long noncoding RNAs as Organizers of Nuclear Architecture

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