Environmental Design Considerationsfor Somatic Embryogenesis

Hoshino, Takanori; Cuello, Joel L.

doi:10.1007/7089_035

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…The cultures placed under complete darkness did not produce somatic embryos. Hoshino and Cuello (2006) reported similar findings that light quality and intensity is one of the critical Plate 1. Different type of callus derived from cotyledon explants 4 to 6 weeks after culturing.…”

Section: Somatic Embryo Formationsupporting

confidence: 55%

Effects of different combinations and concentrations of growth regulators and photoperiod on somatic embryogenesis of cucumis melo var. flexuosus

Çömlekçioğlu¹,

Mendi²,

Eldoğan³

et al. 2009

Afr. J. Biotechnol.

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The present study describes the effects of medium, explant type and photoperiod on somatic embryogenesis of snake melon (Cucumis melo var. flexuosus). Two different types of explant (cotyledon and hypocotyls) obtained from 3 days old snake melon seedlings and culture conditions were investigated for somatic embryogenesis. Explants were cultured on Murashige and Skoog (1962) (MS) medium supplemented with 23 different combinations of auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; α-naphthalene acetic acid, NAA) and cytokinin (6-benzylaminopurine, BAP; N6-[2isopentyl]adenine, 2iP) either alone or in combination with each other. Embryogenic calluses were obtained from cotyledon explant on MS media supplemented with NAA and 2,4-D alone and combinations with cytokinin. Our findings showed that NAA and 2,4-D did not act synergistically with BAP or 2iP. Callus formation was the same whether BAP or 2iP was added into the media or not. The highest embryo formation was achieved from cotyledon explant which was 20.00±7.94 somatic embryos per petri dish on medium supplemented with 4 mg/l 2,4-D and 0.5 mg/l BAP. The cotyledon and hypocotyls explants cultured on the media with 2 mg/l 2,4-D with 0.5 mg/l BAP showed embryogenic callus formation and number of somatic embryos were 17.33±5.51 and 16.33±3.06 per petri dish, respectively. Auxin was found critical for formation of somatic embryo and at the same time, the two tested cytokinins at any concentration acted synergistically with auxin.

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Section: Somatic Embryo Formationsupporting

confidence: 55%

Effects of different combinations and concentrations of growth regulators and photoperiod on somatic embryogenesis of cucumis melo var. flexuosus

Çömlekçioğlu¹,

Mendi²,

Eldoğan³

et al. 2009

Afr. J. Biotechnol.

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“…Similar results have been obtained by Takamori and Cuello [28] in D. carota embryogenic calli using light-emitting diodes (LEDs). In general, red light promotes and blue light inhibits the induction of SE [2]. However, the effect of light quality on the growth of embryogenic tissue can be genotype dependent as has been demonstrated in Norway spruce [29], Cucumis melo L. [30], and Arabidopsis thaliana [31].…”

Section: Introductionmentioning

confidence: 93%

“…Different factors modify the SE response of various species, such as carbon and nitrogen sources, growth regulators, light-dark regimen, and the source of explant [2,3].…”

Section: Introductionmentioning

confidence: 99%

Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

2008

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The concentration of free and bound polyamines was studied during the somatic embryogenesis induction process in Coffea canephora explants. In the present study we show that when the induction of somatic embryogenesis in C. canephora is carried out under light conditions and in the presence of the plant growth regulator, benzylaminopurine, a cytokinin, a faster response to induction is obtained. In the darkness, the response is delayed for more than 20 days, and the number of embryos is smaller. In the absence of benzylaminopurine no embryogenic response was observed. The pronounced changes in the levels of putrescine, spermidine, and spermine, both free and bound, found in C. canephora suggest that a close correlation exists between polyamine biosynthesis and somatic embryogenesis in C. canephora during a period of cellular differentiation associated with the induction of somatic embryogenesis.

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“…Somatic embryos were first induced from transgenic plants[25]. Somatic embryos were then treated with abscisic acid (ABA) to induce desiccation tolerance[50] [52][53] [54][55]. The embryos that acquired desiccation tolerance were subjected to water loss process.…”

mentioning

confidence: 99%

Recent Progress of Transgenic Technology Development for Alfalfa

Liu¹,

Aung²,

Hannoufa³

et al. 2018

AJPS

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Alfalfa (Medicago sativa L.) is an important leguminous crop worldwide and it has important roles in different aspects of the agriculture system, including livestock feed, crop soil conservation, and improvement of soil nitrogen supply. As a perennial crop, alfalfa breeding for trait improvement takes longer periods of time compared to many other crops, therefore, genetic engineering is a faster route for alfalfa trait modification and improvement. Alfalfa was a first crop in which somatic embryogenesis was developed. Alfalfa was also a pioneering crop in which transgenic technology was developed and applied for trait improvement. Transgenic technology in alfalfa has since advanced steadily in various areas. In this review, we update the recent research progress in alfalfa genetic engineering, focusing on genetic transformation and use of transgenic technology for trait improvement and new trait development.

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Environmental Design Considerationsfor Somatic Embryogenesis

Cited by 5 publications

References 23 publications

Effects of different combinations and concentrations of growth regulators and photoperiod on somatic embryogenesis of cucumis melo var. flexuosus

Effects of different combinations and concentrations of growth regulators and photoperiod on somatic embryogenesis of cucumis melo var. flexuosus

Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

Recent Progress of Transgenic Technology Development for Alfalfa

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