Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Ferrari, Elena; Tsay, A.; Eggleston, Peyton A.; Spisni, Alberto; Chapman, Martin D.

doi:10.1016/j.jaci.2004.04.028

Cited by 16 publications

(14 citation statements)

References 33 publications

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“…Filters were weighed before and after sample collection. Loaded filters were transported separately packed and stored in a refrigerator (2 to 8°C) until measurements of mite allergen (Der p1), mouse allergen (Mus m1), and rat allergen (Rat n1) by using enzyme-linked immunosorbent assay (ELISA) 16,17) .…”

Section: Dust Samplesmentioning

confidence: 99%

Study of risk factors for atopic sensitization, asthma, and bronchial hyperresponsiveness in animal laboratory workers

Simoneti

Freitas

Barbosa

et al. 2016

Journal of Occupational Health

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al.: Animal laboratory workers: risks for allergy and asthma associated with sensitization, asthma, or BHR. Conclusion: Exposure to laboratory animals was associated with atopic sensitization. However, we did not find a cutoff allergen concentration that increased the risk for sensitization. Duration of exposure seems to be more relevant to sensitization than concentration of allergens in dust. (J Occup Health 2016; 58: 7−15) Key words: Animal experimentation, Atopy, Bronchial hyperreactivity, Occupational disease Workers exposed to laboratory animals have a high risk of developing allergic reactions 1,2) . Rats and mice are the most common etiology of laboratory animal allergy because they are the most commonly used animals in research 3) . The high prevalence of this condition became evident from epidemiological crosssectional studies conducted in the 1980s 1,4) . Eleven to 44% of individuals who work with laboratory animals have reported allergic symptoms. These symptoms can range from skin reactions, nasal congestion, rhinorrhea, sneezing, and conjunctivitis, to severe asthma. Allergic rhinitis and allergic conjunctivitis symptoms are the most commonly reported symptoms. Among workers who become symptomatic, 4 to 22% have occupational asthma 2) . In a cohort study of 342 laboratory animal workers, quantitative analyses of exposure demonstrated a doseresponse relation between the intensity of exposure to skin symptoms and skin prick tests. Intensity of exposure (divided into 4 categories) was evaluated by quantification of rat urinary aeroallergen. Exposureresponse relations for symptoms were observed among sensitized workers, and for chest and eye/nose symptoms, they were not as clear as for skin symptoms. Two exposure indices were created: one based on the The aim of this estudy was to investigate the influence of allergen exposure levels and other risk factors for allergic sensitization, asthma, and bronchial hyperresponsiveness (BHR) in workers exposed to laboratory animals. Methods: This was a crosssectional study performed at two universities, 123 workplaces with 737 subjects. Dust samples were collected from laboratories and animal facilities housing rats, mice, guinea pigs, rabbits, or hamsters and analyzed by enzyme-linked immunosorbent assay (ELISA) to measure allergen concentrations. We also sampled workplaces without animals. Asthma was defined by both symptoms and BHR to mannitol. The concentrations of allergens were tested for association with a skin prick test, respiratory symptoms, spirometry data, and BHR. This multivariate analysis was performed by using Poisson regression to estimate the relative risk (RR) for the exposed group. Results: Our sample comprised students and workers, with 336 subjects in the nonexposed group and 401 subjects in the exposed group. Sixty-nine subjects (17%) had positive results in the skin prick test for animal allergens in the exposed group; in the nonexposed group, 10 subjects had positive results (3%) (p<0.001). Exposure to laboratory animals over 2.8 years...

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Section: Dust Samplesmentioning

confidence: 99%

Study of risk factors for atopic sensitization, asthma, and bronchial hyperresponsiveness in animal laboratory workers

Simoneti

Freitas

Barbosa

et al. 2016

Journal of Occupational Health

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“…The ELISA kit from Indoor Biotechnologies, Inc, was developed from rabbit polyclonal antibodies to recombinant Mus m 1, whereas the one from Greer Laboratories, Inc, was developed from rabbit polyclonal antibodies to Mus m 1 purified from mouse urine. 13,14 In all analyses the level of significance was set at a P value of .05. Because the purpose of this study was to explore associations rather …”

Section: Statistical Analysesmentioning

confidence: 99%

Exposure to indoor allergens in day-care facilities: Results from 2 North Carolina counties

Arbes

Sever

Mehta

et al. 2005

Journal of Allergy and Clinical Immunology

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“…The antibodies used in these systems have been well defined and used for many years. [2][3][4][5][6][7][8][9] However, the limitations of ELISA procedures are an impediment for large studies of exposure assessment, for prospective studies and, especially, for studies involving multiple allergens.…”

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confidence: 99%

High-throughput fluorescent multiplex array for indoor allergen exposure assessment

Earle

King

Tsay

et al. 2007

Journal of Allergy and Clinical Immunology

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Environmental detection of mouse allergen by means of immunoassay for recombinant Mus m 1

Cited by 16 publications

References 33 publications

Study of risk factors for atopic sensitization, asthma, and bronchial hyperresponsiveness in animal laboratory workers

Study of risk factors for atopic sensitization, asthma, and bronchial hyperresponsiveness in animal laboratory workers

Exposure to indoor allergens in day-care facilities: Results from 2 North Carolina counties

High-throughput fluorescent multiplex array for indoor allergen exposure assessment

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