The object of this study was the development of a new water quality index called the Ecological Water Quality Index (EWQI) which is flexible enough to represent the ecological status of surface waters by assessing chemical quality based on supranational legislation. Eleven variables including nutrients, salts and total parameters were selected as index components. Threshold values were assigned to seven water-quality classes as defined by the legislation and were used to develop mathematical equations to convert observed values to index scores. Depending on the ecological importance of the parameters, weights were assigned to each variable and then a weighted sum method was performed to aggregate sub-indices. The applicability of the method was demonstrated in two basins located in Turkey. Factor analysis was applied to optimize the index component selection process. Several alternatives were tested to comprise at least one variable from each defined factor class (e.g. salinity content) to calculate an index score representing ecological status. Results showed that there were slight differences between index scores of the various tested alternatives. These differences did not cause misclassification of surface waters. The study showed that the EWQI method combined with factor analysis could be a practical and efficient way to represent the ecological status of surface waters.