2017
DOI: 10.3391/mbi.2017.8.1.09
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Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting

Abstract: Harbours are known introduction foci of marine alien species. They act as recipients of new introductions and as sources for regional spread. We report on subtidal surveys of fouling communities from 14 harbours along the coastline of South Africa that were used to identify predictors of high alien species numbers in support of prioritisation of monitoring actions by authorities. The harbours varied in nature from large, international shipping hubs to small, regional fishing harbours and recreational marinas. … Show more

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Cited by 87 publications
(84 citation statements)
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“…We sampled five 2 L water samples from each pond in autumn 2016, but timing and/or sampling effort may have been inappropriate. A seasonal effect on common carp eDNA detection was observed, where spring sampling generated higher eDNA concentration and detection rates due to greater common carp activity (Turner et al., ) and density (Hinlo, Furlan, Suitor, & Gleeson, ). As water sampling did not coincide with fyke netting (spring 2016) in our study, eDNA concentration may not reflect CPUE estimates.…”
Section: Discussionmentioning
confidence: 99%
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“…We sampled five 2 L water samples from each pond in autumn 2016, but timing and/or sampling effort may have been inappropriate. A seasonal effect on common carp eDNA detection was observed, where spring sampling generated higher eDNA concentration and detection rates due to greater common carp activity (Turner et al., ) and density (Hinlo, Furlan, Suitor, & Gleeson, ). As water sampling did not coincide with fyke netting (spring 2016) in our study, eDNA concentration may not reflect CPUE estimates.…”
Section: Discussionmentioning
confidence: 99%
“…We performed a 10‐fold serial dilution of the gBlocks ® fragment to generate a six‐point standard curve that ranged from 10 6 to 10 copies/μl. eDNA samples were compared to these known concentrations for quantification (Hinlo, Furlan, Suitor, & Gleeson, ). Each standard was replicated five times on each qPCR plate.…”
Section: Methodsmentioning
confidence: 99%
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“…), but in others the relationship is unclear (Hinlo et al. ), likely because of sampling techniques (Wilcox et al. ), environmental conditions, and physiological processes of the study organisms.…”
mentioning
confidence: 99%