Environmental Enrichment Blunts Ethanol Consumption after Restraint Stress in C57BL/6 Mice

Marianno, Priscila; Abrahao, Karina Possa; Camarini, Rosana

doi:10.1371/journal.pone.0170317

Cited by 41 publications

(22 citation statements)

References 51 publications

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“…Importantly, some recent studies have shown that social and EE reduces EtOH preference and binge-like EtOH drinking in adult male C57BL/6 mice as measured by a modified, 2BC, DID task. Thus, C57BL/6 mice living in continuous (24 h) or restricted (3 h) EE conditions consumed less EtOH than control mice over 24 h in a DID-2BC procedure after acute stressful conditions (Marianno et al, 2017 ), and C57BL/6 mice group-housed for 40 days immediately after weaning showed lower binge-like EtOH drinking in a DID-2BC task than isolated-housed mice during the same developmental period (Lopez et al, 2011 ). Moreover, providing EE was sufficient to counteract high binge-like EtOH intake triggered by chronic social isolation in C57BL/6J mice (Lopez and Laber, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Environmental Enrichment During Adolescence Acts as a Protective and Therapeutic Tool for Ethanol Binge-Drinking, Anxiety-Like, Novelty Seeking and Compulsive-Like Behaviors in C57BL/6J Mice During Adulthood

Rodríguez-Ortega

Fuente

Amo

et al. 2018

Front. Behav. Neurosci.

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Repetitive drug/ethanol (EtOH) binge-like consumption during pre-addictive stages favors a transition to addiction in vulnerable organisms. Experimental evidence points to the therapeutic and preventive effects of environmental enrichment (EE) on drug and EtOH addiction; however, little is known regarding EE modulation of binge-like consumption in non-dependent organisms. Here, we explore the impact of early EE on binge-like EtOH consumption: (1) we test whether early EE exposure prevents binge-like EtOH intake (20% v/v) in adult mice under an intermittent drinking in the dark (iDID) schedule; (2) we evaluate the therapeutic effects of EE housing conditions on binge-like EtOH consumption in adult animals; and (3) we compare novelty-seeking and compulsive-like behaviors, and anxiety-like behavior, as measured by the Hole Board (HB) and Elevated Plus Maze (EPM) tests, respectively, in adult EE/standard environment (SE) animals. Adolescent (postnatal day 28; PND28) mice were randomly allocated to two housing conditions (4 animals/cage): EE or SE. At PND67 all the animals were exposed to a schedule of EtOH binge-like iDID. On PND92 half of the animals in each environmental condition (EE and SE) were randomly allocated to two subgroups in a crossover design, where environmental conditions were kept similar to those previously experienced or switched, finally leading to four experimental conditions: EE-EE, EE-SE, SE-SE, and SE-EE. EtOH binge-like consumption continued until PND140, when EPM and HB tests were finally conducted. The main observations were: (1) EE-reared mice showed lower EtOH binge-like intake than SE-reared mice during adulthood, which supports a protective role for EE. (2) when adult EtOH drinking SE-reared mice were switched to EE conditions, a reduction in EtOH binge-like consumption was observed, suggesting a therapeutic role for EE; however, losing EE during adulthood triggered a progressive increase in EtOH binge-like intake. Moreover, (3) EE-housed adult animals with long-term exposure to EtOH binge-drinking showed lower anxiety-like, compulsive-like, and novelty-seeking behaviors than SE-housed mice, irrespective of the specific housing conditions during adolescence. We discuss the primary impact of EE on anxiety-like neurobehavioral brain systems through which it secondarily modulates EtOH binge-like drinking.

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Section: Introductionmentioning

confidence: 99%

Environmental Enrichment During Adolescence Acts as a Protective and Therapeutic Tool for Ethanol Binge-Drinking, Anxiety-Like, Novelty Seeking and Compulsive-Like Behaviors in C57BL/6J Mice During Adulthood

Rodríguez-Ortega

Fuente

Amo

et al. 2018

Front. Behav. Neurosci.

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“…In addition to improving psychological and physiological well-being in otherwise healthy animals, EE may limit behavioral, cognitive and biological disturbances provoked by stressful experiences. For instance, EE reduced anxiety-like behaviors and prevented cognitive impairments ordinarily elicited by acute and chronic stressors (Cordner and Tamashiro, 2016 ; Bahi, 2017 ; Marianno et al, 2017 ; Dandi et al, 2018 ). Mice housed in EE also exhibited limited corticosterone elevations and neuronal activation after stressor exposure (Branchi et al, 2013 ; Reichmann et al, 2013 ; Mesa-Gresa et al, 2016 ), indicating that housing conditions may modulate the impact of external stressors on hormonal and brain functions.…”

Section: Introductionmentioning

confidence: 99%

Post-weaning Environmental Enrichment in Male CD-1 Mice: Impact on Social Behaviors, Corticosterone Levels and Prefrontal Cytokine Expression in Adulthood

McQuaid

Dunn

Jacobson‐Pick

et al. 2018

Front. Behav. Neurosci.

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Environmental enrichment is typically associated with enhanced well-being, improved cognitive function and stress resilience. However, in some instances grouping adult male mice in enriched conditions promoted a stressful environment, which resulted in elevated endocrine, monoamine and inflammatory outcomes in response to subsequent stressor exposure. The current investigation examined whether raising male mice in an enriched environment (EE) would modulate social and anxiety-like behaviors in early adulthood and influence brain expression of pro-inflammatory cytokines and brain-derived neurotrophic factor (BDNF). Immediately after weaning (postnatal day [PD] 21), CD-1 male mice were housed with their siblings (3/cage) for 6 weeks in an EE or a standard (SE) environment. Body weights and aggressive interactions were monitored weekly. Social avoidance behaviors in the social interaction test and anxiety-like behaviors in the elevated-plus maze were examined in early adulthood. Ninety minutes following the behavioral tests, mice were sacrificed and a blood sample and the prefrontal cortex (PFC) were collected for the determination of plasma corticosterone levels as well as cytokine and BDNF mRNA expression. Mice raised in an EE exhibited more wounds and gained less weight than mice housed in a SE. Enriched mice also spent a greater amount of time in proximity of a social target in the social interaction test and made fewer transitions into the closed arms of the elevated-plus maze. Interestingly, the elevated plasma corticosterone and upregulated prefrontal interleukin (IL)-1β expression observed after the social interaction test among the SE mice were not apparent among those housed in an EE. Enrichment also increased prefrontal BDNF expression, especially among mice that experienced the social interaction test. These results suggest that although raising male mice in an EE may elicit aggressive interactions between sibling cage-mates (as indicated by a high number of wounds), this environment also enhances social behaviors and limits the corticosterone and cytokine impacts of mild social stressors encountered in early adulthood.

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“…Other work, however, reported lower ethanol consumption and lower ethanol-induced CPP after EE in rats that were selected for high ethanol preference 19 or spontaneous hypertension 20 . More recent studies demonstrated that EE decreased ethanol consumption after restraint stress 21 and that EE blocked the development and expression of ethanol-induced behavioral sensitization in peri-adolescent Swiss mice 12 . EE also decreased BDNF levels in the PFC, but not in hippocampus.…”

Section: Introductionmentioning

confidence: 99%

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

Pautassi

Suarez

Hoffmann

et al. 2017

Sci Rep

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Environmental enrichment (EE) provides a non-pharmacological tool to alter drug-induced reward, yet its effects on ethanol-induced reward remain controversial. We analyzed adolescent vs. adult (mice) differences in the influence of EE on ethanol-induced conditioned place preference (CPP). The effects of these treatments on brain-derived neurotrophic factor (BDNF) levels in the prefrontal cortex were examined in a separate group of animals. Ethanol-induced CPP was found in adults, and it was similar in EE and in animals reared under standard housing conditions (SC). Adolescents kept under EE, but not those in SC, exhibited CPP. Among SC, but not among EE, adolescents, BDNF levels were significantly lower in those treated with ethanol than in those given vehicle. These results indicate that, compared to adults, adolescent exhibited reduced sensitivity to ethanol’s rewarding effects, yet the youth but not the adults exhibited sensitivity to the promoting effect of EE upon CPP by ethanol. Ethanol significantly reduced BDNF levels in adolescents reared under standard housing conditions, but not in adult mice nor in adolescents given EE housing conditions. The present results add to the plethora of adolescent-specific responses to ethanol or to environmental stimuli that may put the youth at risk for escalation of ethanol intake.

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Environmental Enrichment Blunts Ethanol Consumption after Restraint Stress in C57BL/6 Mice

Cited by 41 publications

References 51 publications

Environmental Enrichment During Adolescence Acts as a Protective and Therapeutic Tool for Ethanol Binge-Drinking, Anxiety-Like, Novelty Seeking and Compulsive-Like Behaviors in C57BL/6J Mice During Adulthood

Environmental Enrichment During Adolescence Acts as a Protective and Therapeutic Tool for Ethanol Binge-Drinking, Anxiety-Like, Novelty Seeking and Compulsive-Like Behaviors in C57BL/6J Mice During Adulthood

Post-weaning Environmental Enrichment in Male CD-1 Mice: Impact on Social Behaviors, Corticosterone Levels and Prefrontal Cytokine Expression in Adulthood

Effects of environmental enrichment upon ethanol-induced conditioned place preference and pre-frontal BDNF levels in adolescent and adult mice

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