Environmental Factors Modulating Indole-3-Acetic Acid Biosynthesis by Four Nitrogen Fixing Bacteria in a Liquid Culture Medium

Xa, Le Thi; Nghĩa, Nguyễn Khởi; Tecimen, Hüseyin Barış

doi:10.32526/ennrj/20/202100233

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…The use of levels and salt stress point out an intriguing reason that raising the salt concentration over 1% to 5% lowers the quantity of generated IAA [44]. The type of carbohydrate source has an impact on IAA production.…”

Section: Resultsmentioning

confidence: 99%

Relief from Salt Stress by Plant Growth-Promoting Bacteria in Hydroponic Leaf Lettuce (Lactuca sativa L.)

Nurhidayati,

Arifiyanto,

Saputro

et al. 2023

Pol. J. Environ. Stud.

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Indonesia is a large archipelago with a long coastline. Climate change and saltwater intrusion both endanger freshwater availability as a source of drinking water and agricultural resources. Therefore, the saline and brackish water demand for irrigated farm goods should be considered a method for producing salt-resistant crop products. This study aimed to monitor Indole-3-acetic acid (IAA) and biofilm by selected bacterial isolates and its impact on the growth of lettuce (Lactuca sativa L.) during salt stress. Several isolates are employed such as Streptomyces hygroscopicus GGF4-i18, Streptomyces sp. AB8, Micrococcus luteus, Serratia marcescens MBC1, and Streptomyces hygroscopicus subsp. Jinggangensis InaCC A497. A 1.0 percent L-tryptophan is used as a precursor of IAA. The impact of IAA and biofilm produced by bacteria was demonstrated by inoculating bacterial isolates on Lactuca sativa (lettuce) plants in the hydroponic system. The nutrient film technique (NFT) is the selected hydroponic cultivation technique. The result shows that Streptomyces genera were able to maintain higher IAA hormone production within a week. On the other hand, Serratia marcescens MBC1 and Micrococcus luteus tended to have stable IAA levels. The administration of MBC1 strain bacteria increased lettuce growth as measured by the number of leaves and leaf length, compared to isolate AB8, i18, and Micrococcus luteus. However, the growth was not greater than the control treatment, which grew in fresh water, when it was observed in root length. The ability of bacterial isolates to tolerate salt levels is needed, in addition to the capacity to produce IAA to help plants survive and grow in brackish water media. In nature, the interaction between plants and microbes does not take place alone. In this study, the treatment was still given in the form of a bacterial monoculture to plant. In the future, it is necessary to develop how the treatment is given in the form of a consortium and consider other

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Section: Resultsmentioning

confidence: 99%

Relief from Salt Stress by Plant Growth-Promoting Bacteria in Hydroponic Leaf Lettuce (Lactuca sativa L.)

Nurhidayati,

Arifiyanto,

Saputro

et al. 2023

Pol. J. Environ. Stud.

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“…Perbedaan jumlah konsentrasi IAA yang diproduksi bergantung pada jenis tanaman, lokasi dan faktor lingkungan. Selain itu, efek pemberian konsentrasi triptofan, suhu, pH, salinitas dan lamanya waktu inkubasi juga termasuk faktor penentu jumlah IAA yang diproduksi oleh bakteri (Napitupulu et al, 2019;Xa et al, 2022). Bagian organ tanaman juga menentukan kemampuan bakteri endofit dalam menghasilkan IAA.…”

Section: Diskusiunclassified

KARAKTERISASI DAN POTENSI BAKTERI ENDOFIT AKAR KOPI (Coffea sp) SEBAGAI PENGHASIL INDOLE ACETIC ACID (IAA)

Rizkiana Pratiwi,

Mega Suryani,

Adi Wira Prasetya

et al. 2024

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The Endophytic bacteria as a biofertilizer agent can minimize the use of chemicals from synthetic fertilizers. The aim of this research was to study the density, diversity and ability of endophytic bacteria from coffee plants that have the potential to produce IAA. Root samples were obtained from coffee plants in UB Forest (UBF), Sumberwangi, Malang. The root samples were surface sterilized and then crushed with a blender. Endophytic bacteria were isolated and tested for their potential to produce IAA. The results obtained were 18 isolates of IAA-producing bacteria. KAA6 Isolate were produced the highest concentration of IAA of 10.73 ppm at 48 hours of incubation followed by KBA3 isolate which produced 12.78 ppm of IAA at 72 hours of incubation. The potential bacteria can be further optimized to be used as an eco-friendly biofertilizer agent.

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“…Khiangte & Lalfakzuala (2021) added that Regulatory proteins such as TyrR, PigP, and quorum sensing-related genes have been identi ed as important regulators of IAA production in bacteria. Apart from that, the growth phase of bacterial isolates is also a determining factor in IAA production(Nghia & Tecimen 2022). This may be the reason why some isolates in this study did not appear to produce IAA.…”

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confidence: 99%

The PGPR Revolution: Empowering Porang Plant Growth with Cellulolytic-Hydrolizing, Nitrogen-Fixing, and Phosphate-Solubilizing Prodigies

Masniawati,

Johannes,

Yusran

et al. 2024

Preprint

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Porang rhizosphere harbors a diverse range of bacteria, which play pivotal roles in driving essential soil processes that, in turn, regulate the overall functionality of terrestrial ecosystems. This study primarily focuses on the PGPR (Plant Growth-Promoting Rhizobacteria) properties of these strains, which possess cellulolytic, nitrogen-fixing, and phosphate-solubilizing abilities. The research was carried out by isolating bacteria from the porang rhizosphere. The isolated bacteria were then tested for their ability to hydrolyze cellulose, fix nitrogen and dissolve phosphate. Apart from that, a capability test was also carried out in producing IAA. Isolate UPc22 consistently exhibited higher cellulolytic activity, as evidenced by both cellulose hydrolyzing efficiency cellulase activity and IAA production. UPn24 demonstrated the most active nitrogen-fixing activity, with consistently higher mean values for both activity efficiency and nitrogenase activity quantification. UPp36 displayed notably high phosphate-solubilization efficiency values in plate assays. In enzymatic assays, isolate UPp36 exhibited the highest activity. Based on that assays, 15 potential isolates were selected for each group. For cellulolytic bacteria, the selected isolates were similar with Rhizobium cellulosilyticum Strain H349, Paenibacillus pinihumi Strain CM6, Stenotrophomonas maltophilia Strain JCM 1988, Paenibacillus cellulositrophicus Strain P-21, and Bacillus thuringiensis Strain RG17-11. For nitrogen-fixing bacteria, the chosen isolates comprise Azotobacter vinelandii Strain PWB, Delftia lacustris Strain R-54356, Bacillus subtilis Strain A14d3B, Pseudomonas stutzeri Strain DSM 5190T, and Klebsiella quasipneumoniae Strain KqPF26. For phosphate-solubilizing bacteria, the selected isolates was similar with Pseudomonas putida Strain GPo1, Bacillus magterium Strain WF6, Bacillus licheniformis Strain 51.5, Klebsiella singaporensis Strain 01A065, and Burkholderia cepacia Strain PRS.

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Environmental Factors Modulating Indole-3-Acetic Acid Biosynthesis by Four Nitrogen Fixing Bacteria in a Liquid Culture Medium

Cited by 3 publications

References 23 publications

Relief from Salt Stress by Plant Growth-Promoting Bacteria in Hydroponic Leaf Lettuce (Lactuca sativa L.)

Relief from Salt Stress by Plant Growth-Promoting Bacteria in Hydroponic Leaf Lettuce (Lactuca sativa L.)

KARAKTERISASI DAN POTENSI BAKTERI ENDOFIT AKAR KOPI (Coffea sp) SEBAGAI PENGHASIL INDOLE ACETIC ACID (IAA)

The PGPR Revolution: Empowering Porang Plant Growth with Cellulolytic-Hydrolizing, Nitrogen-Fixing, and Phosphate-Solubilizing Prodigies

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