Environmental<i>Burkholderia cepacia</i>Complex Isolates from Human Infections

Baldwin, Amy; Mahenthiralingam, Eshwar; Dřevı́nek, Pavel; Vandamme, Peter; Govan, John R. W.; Waine, D.J.; LiPuma, John J.; Chiarini, Luigi; Dalmastri, Claudia; Henry, Deborah A.; Speert, David P.; Honeybourne, D.; Maiden, Martin C. J.; Dowson, Christopher G.

doi:10.3201/eid1303.060403

Cited by 113 publications

(103 citation statements)

References 14 publications

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“…161,[298][299][300] Strains identical to those found in people with CF have also been isolated from onions and onion rot. 301,302 Furthermore, MLST analysis demonstrated that more than 20% of 381 CF isolates of Burkholderia were indistinguishable from strains recovered from the natural environment. 301 B. cepacia complex isolates with the same nucleotide identity at all 7 MLST loci have been isolated from people with CF and from river water, suggesting another possible environmental reservoir.…”

Section: Iiid1 Nonhealthcare Sources: Soil Organic Matter and Watermentioning

confidence: 99%

“…301,302 Furthermore, MLST analysis demonstrated that more than 20% of 381 CF isolates of Burkholderia were indistinguishable from strains recovered from the natural environment. 301 B. cepacia complex isolates with the same nucleotide identity at all 7 MLST loci have been isolated from people with CF and from river water, suggesting another possible environmental reservoir. 302 Finally, B. cenocepacia and Burkholderia vietnamiensis have been isolated from human sewage in the United Kingdom, but genotyping of these isolates was not performed.…”

Section: Iiid1 Nonhealthcare Sources: Soil Organic Matter and Watermentioning

confidence: 99%

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Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

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The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline for CF. During the past decade, new knowledge and new challenges provided the following rationale to develop updated IP&C strategies for this unique population:1. The need to integrate relevant recommendations from evidence-based guidelines published since 2003 into IP&C practices for CF. These included guidelines from the Centers for Disease Control and Prevention (CDC)/Healthcare Infection Control Practices Advisory Committee (HICPAC), the World Health Organization (WHO), and key professional societies, including the Infectious Diseases Society of America (IDSA) and the Society for Healthcare Epidemiology of America (SHEA). During the past decade, new evidence has led to a renewed emphasis on source containment of potential pathogens and the role played by the contaminated healthcare environment in the transmission of infectious agents. Furthermore, an increased understanding of the importance of the application of implementation science, monitoring adherence, and feedback principles has been shown to increase the effectiveness of IP&C guideline recommendations.2. Experience with emerging pathogens in the non-CF population has expanded our understanding of droplet transmission of respiratory pathogens and can inform IP&C strategies for CF. These pathogens include severe acute respiratory syndrome coronavirus and the 2009 influenza A H1N1. Lessons learned about preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant gram-negative pathogens in non-CF patient populations also can inform IP&C strategies for CF.

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Section: Iiid1 Nonhealthcare Sources: Soil Organic Matter and Watermentioning

confidence: 99%

Section: Iiid1 Nonhealthcare Sources: Soil Organic Matter and Watermentioning

confidence: 99%

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

View full text Add to dashboard Cite

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“…All BCC species can be isolated from clinical samples as well as from the natural environment, which could be acting as a constant "reservoir" for infectious BCC pathogens [16]. Furthermore, most BCC species include isolates of both clinical and environmental origin showing the same sequence type (ST) by multi locus sequence typing (MLST), i.e.…”

Section: Pathogenicity Of Burkholderia Speciesmentioning

confidence: 99%

Pathogenicity and biotechnological applications of the genus Burkholderia

2011

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Bacteria belonging to the genus Burkholderia are well known for their adaptability to habitats as diverse as freshwater sediments, lungs of cystic fibrosis patients and plant tissues. This genus includes also plant, animal and human pathogenic species, such as Burkholderia glumae, Burkholderia pseudomallei and the Burkholderia cepacia complex. Over the past few years, several newly discovered non-pathogenic plant associated Burkholderia species have raised particular interest for their potential use in plant growth promotion, biocontrol of plant pathogens, phytoremediation and xenobiotics degradation. Highlights from recent studies on the taxonomy, ecology and pathogenicity of different species of the Burkholderia genus are presented with the aim to evaluate their potential use in biotechnology.

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“…Evidence for this assumption was presented in a recent transcriptomic study of the oral bacterium Porphyromonas gingivalis, which, when exposed to H 2 O 2 , demonstrated concerted overexpression of one IS family (4). The aims of our study were (i) to characterize the distribution of selected IS elements in the Czech epidemic strain CZ1 (designated ST-32 by MLST [1]), (ii) to evaluate their possible link to the heterogeneous fingerprints of CZ1 generated by PFGE, and (iii) to examine whether oxidative stress conditions promoted the movement of IS elements within the genomes of strain CZ1 and the sequenced B. cenocepacia strain J2315 (ST-28).…”

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confidence: 99%

Oxidative Stress of Burkholderia cenocepacia Induces Insertion Sequence-Mediated Genomic Rearrangements That Interfere with Macrorestriction-Based Genotyping

et al. 2010

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Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the CzechRepublic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. The clonality of the isolates was evident by multilocus sequence typing; however, fingerprinting profiles obtained by pulsed-field gel electrophoresis (PFGE) showed substantial band variability. We investigated whether the PFGE pattern diversity resulted from genomic rearrangements mediated by insertion sequences (IS); in addition, we determined whether stressful growth conditions altered the transposition activity of these IS. DNA probes for IS commonly found in B. cenocepacia were designed using the B. cenocepacia J2315 genome. Southern hybridization analysis of ST-32 isolates demonstrated diversity in both the copy number and the insertion site for a homologue of ISBcen20. Movement of the ISBcen20 homologue was detected when the ST-32 isolate CZ1238 was exposed to oxidative stress (growth in the presence of H 2 O 2 ). PFGE analysis of CZ1238 derivatives exposed to oxidative stress demonstrated genomic rearrangements. Interestingly, when the closely related B. cenocepacia strain J2315 was exposed to oxidative stress, no movement of ISBcen20 was detected. Since frameshift mutations are present within the transposases of all copies of this IS in J2315, our data suggest that the transposase is inactive. In summary, we have demonstrated for the first time that IS movement can be mediated by oxidative stress and can lead to genomic rearrangements in the CF pathogen B. cenocepacia. These IS movements may alter the PFGE fingerprints of isolates that are clonal by other typing methods.

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EnvironmentalBurkholderia cepaciaComplex Isolates from Human Infections

Cited by 113 publications

References 14 publications

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Pathogenicity and biotechnological applications of the genus Burkholderia

Oxidative Stress of Burkholderia cenocepacia Induces Insertion Sequence-Mediated Genomic Rearrangements That Interfere with Macrorestriction-Based Genotyping

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