The main goal of this study is to present the life cycle assessment results of an innovative closed-loop production system, called an agriponic system, used for producing tomatoes. In the study, this new system is presented, as well as its related environmental impacts generated for the production of the tomatoes. A life cycle assessment (according to ISO 14040) was applied to it, from seedling purchase and planting to harvest, using a functional unit of 1 ton of cherry tomatoes produced. SimaPro 9.3.0.3 software and the Ecoinvent database were used to analyze five impact categories. Plant growth emerged as the process unit with the highest impact, particularly for the ozone depletion potential (ODP), with a value of 0.00056 kgCFC-11eq, and for photochemical oxidation (POCP), with a value of 0.0784 kgC2H4eq impact categories. Greenhouse climate management presented a significant impact to the acidification potential (AP), with a value of 1.021 kgSO2eq. Conversely, the phases of plant transplanting, harvesting, and crop disposal had positive impacts for all impact categories considered in the study, because they were very low. In conclusion, agriponic greenhouse tomato production is a sustainable process. This is due to fewer pesticides that are used, and to nutrient solution reuse.