The overarching goal of agricultural sciences is to optimize production technology to rationalize the use of production resources, energy, and space. Due to its high fertilization and water requirements, the vine is a plant with a high potential for greenhouse gas (GHG) emissions. The modifying factor in the production technology is plantation management. To reach the assumed goal, a field experiment was conducted in the years 2001–2020, and the following training systems were used: multi-arm fan system (A) trunk height <30 cm, (B) 80 cm, (C) 120 cm, one-side multi-arm, paired planting (D) 120 cm, (E) 140 cm. The total amount of GHGs emitted in vine cultivation was calculated according to ISO 14040 and ISO 14044 standards. The system boundaries were: establishing the plantation, the production and use of fertilizers and pesticides, energy consumption for agricultural treatments, and gas emissions from the soil. The amount of GHG emissions for cultivation using the systems A, B, C ranged from 426.77 to 556.34 kg of CO2-eq Mg of yield−1, while in the case of D and E systems, the value was approx. 304.37 to 306.23 CO2-eq Mg of yield−1. When comparing this stage with total annual emissions related to cultivation (for 1 ha), the amount of emitted GHGs at this stage is from approx. 42% to 58% higher than from annual emission related to cultivation. Concrete poles are the main element related with GHG emission during stage of plantation establishment, from 97 to 98% of emission. In the case of annual production, nitrogen fertilizers are responsible for approx. 36%. Moreover, the results show that systems D and E increased the average annual fruit yield (per 19 years of research) by approx. 68% compared to the A, B, C systems. There was no difference in the yield of plants with different height of shoots in the D and E systems. The “one-side, multi-arm, paired planting system” was characterized by the highest production and environmental efficiency.