This report provides a methodology and requisite data to assess the potential Energy Return on (Energy) Investment (EROI) for nuclear fuel cycle alternatives, and applies that methodology to an example "oncethrough" fuel cycle using low enrichment uranium (LEU) in conventional light water cooled reactors (LWRs). Within the DOE Office of Nuclear Energy Science and Technology -Fuel Cycle Technologies program, the Fuel Cycle Options Campaign "performs integrating analyses of nuclear energy and fuel cycle systems to inform fuel cycle R&D, programmatic decisions, strategy formulation, and policy development". Campaign objectives include development of relevant fuel cycle metrics, and development of tools and associated data for analysis of fuel cycle systems. This study represents an extension of a prior evaluation of EROI as a metric for fuel cycle facilities, processes and technologies. That prior study [Simon 2011] addressed the energy return on the addition of fuel recycle to an existing nuclear energy system. Limited to just the addition of fuel recycle, that study did not include all the energy investments required to create, operate and decommission the underlying nuclear fuel cycle, such as uranium mining, fuel fabrication, reactor construction, and used fuel disposition. This extension of the prior work adds these remaining pieces of the fuel cycle to provide a basic evaluation framework and initial data to enable evaluation of EROI for nuclear energy in general. It is intended as a basis for evaluation of alternative fuel cycle options in the future with the addition of pertinent details for other fuel cycle scenarios.In the prior work, a spreadsheet tool was constructed for the specific purpose of this energy input/output analysis of nuclear fuel cycle facilities. This tool allows the user to enter the parameters of the nuclear fuel cycle and assumptions about energy use in reprocessing. In the current analysis, energy consumption for an entire nuclear energy enterprise is considered, including facility construction, materials, facility operation, and decommissioning. The analysis tool has been extended to include the entire fuel cycle for a representative scenario of a once-through LWR nuclear energy system. Energy content data have been developed for an initial representation. In many cases where there is a valid range of plausible energy content, the range is discussed and a representative value selected for demonstration of the evaluation methodology. In this analysis, energy produced is the energy output of power reactors within the fuel cycle. The spreadsheet calculates both Primary EROI and Final EROI for the fuel cycle entered. The EROI is the ratio of the output energy divided by the consumed energy.The intent for this study was to develop the methodology and analysis tool for a complete fuel cycle. It is not the intent of the study to fully explore the wide range of potential energy intensities, or to reconcile the disparate values found in the literature. Representative numbers were used in this ...