Environmental KPC-Producing Escherichia coli Isolates in Portugal

Poirel, Laurent; Barbosa-Vasconcelos, Ana; Simões, Roméo Rocha; Costa, Paulo Martins da; Liu, Winchygn; Nordmann, Patrice

doi:10.1128/aac.05850-11

Cited by 60 publications

(52 citation statements)

References 9 publications

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“…KPC and GES-type carbapenemase producers have been observed in polluted aquatic matrices [4][5][6][7][8][9], although their presence in recreational water was so far unreported. Qnr-encoding genes have already been reported in such an environment [16].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, reports regarding their occurrence in environmental matrices are increasingly frequent [3]. For instance, Klebsiella pneumoniae carbapenemase (KPC)-producing isolates have been recovered from rivers located in France [4], Portugal [5] and Brazil [6] as well as from sewage samples in China [7], Brazil [8] and Austria [9]. Polluted rivers frequently flow to oceans, likely contaminating seawater with MDR bacteria and making these aquatic environments potential vectors of resistance for beach frequenters.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of carbapenemase-producing bacteria in coastal recreational waters

Montezzi

Campana

Corrêa

et al. 2015

International Journal of Antimicrobial Agents

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Occurrence of carbapenemase-producing bacteria in coastal recreational waters

Montezzi

Campana

Corrêa

et al. 2015

International Journal of Antimicrobial Agents

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“…To date, two major sequence types containing bla KPC genes, ST131 and ST410, have been reported. ST131 was detected in China, France, and USA Morris et al 2011;Naas et al 2011); and ST410 was reported in Portugal and Taiwan (Ma et al 2013;Poirel et al 2012). KPC-producing E. coli of ST648 has so far only been reported in China .…”

Section: Discussionmentioning

confidence: 95%

Emergence of KPC-2-producing Escherichia coli isolates in an urban river in Harbin, China

Jiang

et al. 2015

World J Microbiol Biotechnol

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Three KPC-2-producing Escherichia coli (E1, E2, and E3) were recovered from water samples of an urban river in the city of Harbin, China. Antimicrobial susceptibility was determined by broth microdilution. Molecular characterization and genetic relatedness of the isolates were determined by polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and PCR-directed phylotyping. Plasmids were analyzed by conjugation, S1-PFGE, Southern blotting and PCR-based replicon typing (PBRT). The genetic environment of the bla KPC-2 gene was determined using PCR and sequencing. PCR analyses revealed that the E1 isolate carried the bla KPC-2, bla CMY-2, bla TEM-1, bla CTX-M-14, and qnrB2 genes and belonged to sequence type ST410, phylogenetic type A; the E2 isolate was assigned to ST131-B2 and carried the bla KPC-2, bla TEM-1, bla CTX-M-3, bla DHA-1, aac(6')-Ib-cr, and qnrS1 genes; while the E3 isolate was of ST648-D and possessed bla KPC-2, bla TEM-1, bla OXA-1, bla CTX-M-15, armA, and aac(6')-Ib-cr genes. PFGE demonstrated that each of the three KPC-2-producing E. coli isolates exhibited an individual XbaI patterns. The bla KPC-2 gene was located on plasmids of 60-140 kb with IncA/C, IncN, or non-typeable replicon types. The genetic environment of bla KPC-2 of the three strains was consistent with the genetic structure of bla KPC-2 on the plasmid pKP048.

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“…were found (Picao et al, 2013). A single isolate of carbapenem-resistant A. baumannii (Girlich et al, 2010) and E. coli (Poirel et al, 2012) were isolated from river water in Europe. Different species of carbapenem-resistant bacteria were isolated from drinking water in New Delhi (Walsh et al 2011;Tanner et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the numbers of viable CRBP in wastewater remain to be determined, in order to quantify the influence of wastewater on the spread of CRBP in the environment. Moreover, the temperature at which the CRBP 35-37°C (Girlich et al, 2010;Ferreira et al, 2011;Walsh et al, 2011;Galler et al, 2014;Tanner et al, 2015) or was not specified at all (Chagas et al, 2011;Poirel et al, 2012;Picao et al, 2013;Zhang et al, 2013). Cultivation at 37°C allows for the isolation of intrinsically carbapenem-resistant bacteria which are autochthonous populations in environmental samples.…”

Section: Introductionmentioning

confidence: 99%