Environmental lead effects on gene expression in oral epithelial cells

Peyyala, Rebecca; Emecen‐Huja, Pinar; Ebersole, Jeffrey L.

doi:10.1111/jre.12594

Cited by 8 publications

(6 citation statements)

References 111 publications

(170 reference statements)

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“…These membrane proteins are fundamental to many biological processes, such as cell division, control of cell volume, and control of which substances pass through the cell membrane. These results may be relevant because of the role of these proteins in the bacterial resistance to antibiotics [42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

Gómez¹,

Bacilio-Amaranto²,

Torres³

et al. 2020

Preprint

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Background:To successfully colonize the oral cavity, bacteria must adhere directly or indirectly to the oral surfaces available. Fusobacterium nucleatum plays an important role in the development of the oral biofilm community due to its broad adhesion capabilities, serving as a bridge between the members of the oral biofilm community that cannot be directly joined together. The purpose of this study was to identify and localize the proteins associated with the formation of biofilms of Streptococcus gordonii and F. nucleatum. Methods: Multispecies biofilms were identified by amplification of the srtA and radD genes by real-time PCR. Biofilm cells cultured with sucrose were counted. The protein concentrations in the membrane and cytoplasmic fractions were quantified by western blot. Results: The proteins HSP40 and GAPDH were detected in the cytoplasmic fraction of biofilm and F. nucleatum, respectively. The available anti-GAPDH antibody is specific for GAPDH produced by F. nucleatum, which indicated the coaggregation of F. nucleatum on S. gordonii. Conclusions: HSP40 was only detected in the cytoplasmic fraction of the biofilms, making it one of the essential proteins for adherence. This complex set of interactions could have critical implications for the formation and maturation of oral biofilms in vivo and could provide clues to the mechanism behind the distribution of organisms within the human oral cavity.

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Section: Discussionmentioning

confidence: 99%

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

Gómez¹,

Bacilio-Amaranto²,

Torres³

et al. 2020

Preprint

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show abstract

“…The cytoplasmic protein profiles of the biofilms harvested after 1, 4, 7, and 10 days of cultivation remained unchanged over time, demonstrating that the examined proteins are at constant levels throughout different aspects of biofilm formation, such as cell division, control of cell volume, and control of which substances pass through the cell membrane. These results may be relevant because of the role of these proteins in bacterial resistance to antibiotics [42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Identification of Proteins Associated with the Formation of Oral Biofilms

Gómez

Amaranto

Torres

et al. 2021

Pesqui. Bras. Odontopediatria Clín. Integr.

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Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion.

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“…CCL20 is a 70-amino-acid chemokine that attracts immature dendritic cells and T cells via the chemokine receptor CCR6. It plays a role in the specific differentiation of lymphocytes, such as developing Th17 and Treg cells that migrate into inflamed periodontal tissues [ 98 , 99 ]. Ghosh et al detected that primary oral epithelial cells release CCL20 as a reaction to F. nucleatum [ 100 ].…”

Section: F Nucleatum and Immune Responsesmentioning

confidence: 99%

Pathogenic Mechanisms of Fusobacterium nucleatum on Oral Epithelial Cells

Groeger¹,

Zhou²,

Ruf³

et al. 2022

Front. Oral. Health

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Periodontitis is an oral chronic inflammatory disease and may cause tooth loss in adults. Oral epithelial cells provide a barrier for bacteria and participate in the immune response. Fusobacterium nucleatum (F. nucleatum) is one of the common inhabitants of the oral cavity and has been identified as a potential etiologic bacterial agent of oral diseases, such as periodontitis and oral carcinomas. F. nucleatum has been shown to be of importance in the development of diverse human cancers. In the dental biofilm, it exhibits a structural role as a bridging organism, connecting primary colonizers to the largely anaerobic secondary colonizers. It expresses adhesins and is able to induce host cell responses, including the upregulation of defensins and the release of chemokines and interleukins. Like other microorganisms, its detection is achieved through germline-encoded pattern-recognition receptors (PRRs) and pathogen-associated molecular patterns (PAMPs). By identification of the pathogenic mechanisms of F. nucleatum it will be possible to develop effective methods for the diagnosis, prevention, and treatment of diseases in which a F. nucleatum infection is involved. This review summarizes the recent progress in research targeting F. nucleatum and its impact on oral epithelial cells.

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Environmental lead effects on gene expression in oral epithelial cells

Abstract: The results provided new information on a portfolio of genes expressed by oral epithelial cells, targeted substantial increases in an array of immune-related genes post-biofilm challenge, and a focused impact of environmental lead on these induced responses.

Cited by 8 publications

References 111 publications

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

Identification and localization of proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms

Identification of Proteins Associated with the Formation of Oral Biofilms

Pathogenic Mechanisms of Fusobacterium nucleatum on Oral Epithelial Cells

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