Laboratories globally contribute significantly to consumption of resources, greenhouse gas emissions, and generation of waste. Shellfish destined for human consumption are required to be tested for the presence of regulated marine biotoxins, that can be harmful to human health. Whilst running the national monitoring program for the detection of biotoxins in shellfish, efforts were made to increase resource efficiencies by reducing waste and energy consumption leading to reduced environmental and financial costs. Methods were verified to allow transitions to more sustainable and environmentally-friendly consumables, replacing plastics with paperboard and glass alternatives, leading to a reduction in the consumption of single-use plastics by 69%. A shift to polystyrene recycling and composting non-toxic shellfish waste led to an overall reduction in non-chemical waste of >95%. Adoption of green analytical chemistry principles to procurement and preparation of chemical solutions led to a reduction in hazardous chemical waste by ~23%. A further reduction in printing (~81%) was achieved by transitioning to digital document control. Strategies to reduce energy consumption through ‘switch off’ campaigns and improved fume hood and cold storage equipment management were also implemented. Fume hood and cold storage equipment energy consumption was reduced by 30%. The strategies implemented could be adopted by other laboratories e.g., monitoring and research laboratories dealing with pharmaceutical, biological, and environmental samples.