Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)

Schnell, Sabine; Bawa-Allah, Kafilat A.; Otitoloju, Adebayo A.; Högstrand, Christer; Miller, Thomas H.; Barron, Leon; Bury, Nicolas R.

doi:10.1016/j.ecoenv.2015.06.012

Cited by 20 publications

(8 citation statements)

References 44 publications

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“…The above limitations of RTgill-W1 cells as a biosensing element led to the development of the primary FIsh Gill Cell culture system (FIGCS) [147,150,151]. FIGCS cells can be cultured at ambient temperatures with no carbon dioxide supplementation.…”

Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

“…FIGCS cells can be cultured at ambient temperatures with no carbon dioxide supplementation. The fact that they reportedly tolerate the application of raw river water on the apical surface, thus mimicking the physiological functionality of live fish, is particularly critical for water biomonitoring paradigms [150,151]. When exposed to uncontaminated natural waters, the FIGCS showed no cell viability loss following a 24-h incubation.…”

Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

“…When exposed to uncontaminated natural waters, the FIGCS showed no cell viability loss following a 24-h incubation. Moreover, the FIGCS has been successfully transported for field testing (approximately 1000 km during a 30-h period) during both laboratory and field-based heavy metal contamination monitoring of rivers [150][151][152]. The development of innovative cell-based systems, such as the FIGCS, offers tremendous opportunities for whole-cell-based biosensors that can exploit ECIS, respirometry as well as fluoresce viability probes as endpoint readouts.…”

Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

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Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives

Wlodkowic

Karpiński

2021

Sensors

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Continuous monitoring and early warning of potential water contamination with toxic chemicals is of paramount importance for human health and sustainable food production. During the last few decades there have been noteworthy advances in technologies for the automated sensing of physicochemical parameters of water. These do not translate well into online monitoring of chemical pollutants since most of them are either incapable of real-time detection or unable to detect impacts on biological organisms. As a result, biological early warning systems have been proposed to supplement conventional water quality test strategies. Such systems can continuously evaluate physiological parameters of suitable aquatic species and alert the user to the presence of toxicants. In this regard, single cellular organisms, such as bacteria, cyanobacteria, micro-algae and vertebrate cell lines, offer promising avenues for development of water biosensors. Historically, only a handful of systems utilising single-cell organisms have been deployed as established online water biomonitoring tools. Recent advances in recombinant microorganisms, cell immobilisation techniques, live-cell microarrays and microfluidic Lab-on-a-Chip technologies open new avenues to develop miniaturised systems capable of detecting a broad range of water contaminants. In experimental settings, they have been shown as sensitive and rapid biosensors with capabilities to detect traces of contaminants. In this work, we critically review the recent advances and practical prospects of biological early warning systems based on live-cell biosensors. We demonstrate historical deployment successes, technological innovations, as well as current challenges for the broader deployment of live-cell biosensors in the monitoring of water quality.

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Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

Section: Biosensing With Vertebrate Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives

Wlodkowic

Karpiński

2021

Sensors

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“…The advantage of these properties in aquatic toxicity testing is that the influx/efflux pathway of organic and inorganic pollutants dissolved in water as well as toxic effects in the fish gill can be assessed precisely, without the extraction phase or resuspension of toxicants in culture medium which is necessary when using immortalised cell lines. Gill cells cultured using the DSI technique has been successfully used to evaluate toxicity of pollutants in freshwater environments (Minghetti et al, 2014;Schnell et al, 2015) and they have also been used to characterise trace element toxicity (Walker et al, 2008) as well as pharmaceutical uptake and efflux (Stott et al, 2015;Chang et al, 2019) in freshwater.…”

Section: Introductionmentioning

confidence: 99%

Cultured rainbow trout gill epithelium as an in vitro method for marine ecosystem toxicological studies

Bawa-Allah

Otitoloju

Högstrand

2021

Heliyon

Self Cite

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Accurate assessment of the toxic potential of waterborne chemicals is vital to pollution control and management in aquatic ecosystems. However, there is a global advocacy for the reduction, replacement, and refinement of the use of whole organisms in chemical screening studies. This has encouraged the development of alternative in vitro and computer-based techniques. In this study we investigated the possibility of optimising cultured rainbow trout gill epithelium to tolerate seawater and its use to assess toxicity of waterborne chemicals. Gill cells were obtained from rainbow trout acclimated to freshwater or to artificial seawater and were cultured in L-15 culture medium supplemented with or without cortisol. Intact gill epithelia were subjected to 20‰, 25‰ or 30‰ artificial seawater for 24 h and cell viability was assessed. The viability of gill cells obtained from freshwater or artificial seawater acclimated fish and grown without cortisol reduced to less than 80% compared to controls. The addition of cortisol to culture medium improved cell viability in seawater with 94%-95% viability compared to controls. The optimised gill cell epithelium was exposed to trace elements at concentrations previously reported as causing 50% response or mortality (EC/LC 50 ) using other cell-based and in vivo studies. Viability of the gill cells were compared to the 50% response or survival reported. The gill cells were found to be more sensitive than other isolated primary seawater-fish cells, having 5%, 16% and 37% survival on exposure to arsenic, cadmium, and lead, respectively. Results from this study has shown that cultured rainbow trout gill epithelia can be optimised to tolerate seawater and can be used in toxicological evaluations of pollutants resuspended in seawater, mimicking marine ecosystem conditions. The optimised gill cell system can serve as a viable in vitro method for marine ecosystem toxicological studies which would facilitate effective pollution control and management.

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“…To illustrate this, a primary fish gill and liver culture were developed from rainbow trout ( Oncorhynchus mykiss ; RT) in the early 1990s ( Pärt et al, 1993 ; Flouriot et al, 1993 ). Recent improvements in the techniques of both the gill ( Maunder et al, 2017 ; Schnell et al, 2016 ; Wood and Pärt, 1997 ) and the liver ( Uchea et al, 2015 , 2013 ; Baron et al, 2012 ) have led to the use of these systems to study pharmaceutical metabolism, as environmental monitoring systems and transport ( Baron et al, 2017 ; Schnell et al, 2015 ; Stott et al, 2015 ). Concurrently, there has been an increase in studies which extrapolate biotransformation data from in vitro to in vivo scenarios ( Cowan-Ellsberry et al, 2008 ; Nichols et al, 2006 ), allowing for the derivation of bioconcentration factors ( Nichols et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Oncorhynchus mykiss

2018

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A novel method for the establishment and long-term maintenance of ex vivo cultures from intestinal regions of the rainbow trout, Oncorhynchus mykiss (Walbaum), is reported. Adherence of cells was observed within hours, epithelial island formation recorded at 48 h and rapid proliferation with confluence achieved between 9-14 days. In addition to metabolic characterisation, basic morphology of growing cells was characterised using histology, immunofluorescence, transmission electron microscopy (TEM) and transepithelial electrical resistance (TEER). Regional differences in intestinal ethoxyresorufin-O-deethylase (EROD) and 7-ethoxycoumarin-O-deethylation (ECOD) activities in these primary grown enterocytes were compared following exposure to model inducers [i.e. α-NF, β-NF, B(a)P] which demonstrated significant differences. Regional differences in dietary uptake and metabolism of contaminants can therefore be studied in this in vitro system to increase our understanding of fundamental processes, while concurrently providing a means to reduce the number of fish required for biological studies in line with the principles of the 3Rs (Reduce, Refine and Replace).This article has an associated First Person interview with the first author of the paper.

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Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)

Cited by 20 publications

References 44 publications

Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives

Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives

Cultured rainbow trout gill epithelium as an in vitro method for marine ecosystem toxicological studies

Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Oncorhynchus mykiss

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