Surface cleaning is a well-known control procedure against the dissemination of microorganisms in the hospital environment. This prospective study, carried out in an intensive care unit over the course of 14 days, describes the cleaning/disinfection conditions of four surfaces near patients. In total, 100 assessments of the surfaces were carried out after they were cleaned. Three methods were used to evaluate cleanliness: a visual inspection, an adenosine triphosphate (ATP) bioluminescence assay and testing for the presence of A inspeção visual não se mostrou medida confiável para avaliar a limpeza das superfícies.Os resultados demonstram que a rotina de limpeza adotada precisa ser revista. no se mostró una medida confiable para evaluar la limpieza de las superficies. Los resultados demostraron que la actual rutina de limpieza precisa ser modificada.