Environmental risk assessment of pesticides in Ethiopia : a case of surface water systems

Teklu, Berhan M.

doi:10.18174/380652

Cited by 10 publications

(16 citation statements)

References 71 publications

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“…The presence of the A301S mutation most likely reflects the long historical use of cyclodienes in malaria vector control . In addition, although Ethiopia banned cyclodienes in 2004, cyclodienes such as endosulfan and chlordane are still detectable in environmental samples from some regions of Ethiopia . In line with Alemayehu et al 2017, all three resistant populations (ASN, CHW and TOL) harbor the kdr mutation L1014F while the N1575Y mutation was absent .…”

Section: Discussionmentioning

confidence: 63%

Genome‐wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with deltamethrin and DDT resistance in Anopheles arabiensis populations from Ethiopia

Simma

Dermauw

Balabanidou

et al. 2019

Pest Management Science

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BACKGROUND: Vector control is the main intervention in malaria control and elimination strategies. However, the development of insecticide resistance is one of the major challenges for controlling malaria vectors. Anopheles arabiensis populations in Ethiopia showed resistance against both DDT and the pyrethroid deltamethrin. Although an L1014F target-site resistance mutation was present in the voltage gated sodium channel of investigated populations, the levels of resistance indicated the presence of additional resistance mechanisms. In this study, we used genome-wide transcriptome profiling by RNAseq to assess differentially expressed genes between three deltamethrin and DDT resistant An. arabiensis field populations -Asendabo, Chewaka and Tolay -and two susceptible strains -Sekoru and Mozambique. RESULTS: Both RNAseq analysis and RT-qPCR showed that a glutathione-S-transferase, gstd3, and a cytochrome P450 monooxygenase, cyp6p4, were significantly overexpressed in the group of resistant populations compared to the susceptible strains, suggesting that the enzymes they encode play a key role in metabolic resistance against deltamethrin or DDT. Furthermore, a gene ontology enrichment analysis showed that expression changes of cuticle related genes were strongly associated with insecticide resistance. Although this did not translate in increased thickness of the procuticle, a higher cuticular hydrocarbon content was observed in a resistant population. CONCLUSION: Our transcriptome sequencing of deltamethrin and DDT resistant An. arabiensis populations from Ethiopia suggests non-target site resistance mechanisms and paves the way for further investigation of the role of cuticle composition in insecticide resistance of malaria vectors.

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Section: Discussionmentioning

confidence: 63%

Genome‐wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with deltamethrin and DDT resistance in Anopheles arabiensis populations from Ethiopia

Simma

Dermauw

Balabanidou

et al. 2019

Pest Management Science

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show abstract

“…64 In addition, although Ethiopia banned cyclodienes in 2004 65 , cyclodienes such as endosulfan and chlordane are still detectable in environmental samples from some regions of Ethiopia. 66, 67 In line with Alemayehu et al 2017, all three resistant populations (ASN, CHW and TOL) harbor the kdr mutation L1014F while the N1575Y mutation was absent. 12 We also identified the L1014F mutation in the deltamethrin and DDT susceptible SEK strain (Table S5).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with pyrethroid resistance in Anopheles arabiensis populations from Ethiopia

Simma

Dermauw

Balabanidou

et al. 2018

Preprint

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BACKGROUND: Vector control is the main intervention in malaria control and elimination strategies. However, the development of insecticide resistance is one of the major challenges for controlling malaria vectors. Anopheles arabiensis populations in Ethiopia showed resistance against both DDT and the pyrethroid deltamethrin. Although a L1014F target-site resistance mutation was present in the voltage gated sodium channel of investigated populations, the levels of resistance and biochemical studies indicated the presence of additional resistance mechanisms. In this study, we used genome-wide transcriptome profiling by RNAseq to assess differentially expressed genes between three deltamethrin and DDT resistant An. arabiensis field populations (Tolay, Asendabo, Chewaka) and two susceptible strains (Sekoru and Mozambique).RESULTS: Both RNAseq analysis and RT-qPCR showed that a glutathione-S-transferase, gstd3, and a cytochrome P450 monooxygenase, cyp6p4, were significantly overexpressed in the group of resistant populations compared to the susceptible strains, suggesting that the enzymes they encode play a key role in metabolic resistance against deltamethrin or DDT.Furthermore, a gene ontology enrichment analysis showed that expression changes of cuticle related genes were strongly associated with insecticide resistance, although this did not translate in increased thickness of the procuticle. CONCLUSION: Our transcriptome sequencing of deltamethrin/DDT resistant An. arabiensis populations from Ethiopia suggests non-target site resistance mechanisms and pave the way for further investigation of the role of cuticle composition in resistance.

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“…This might be due to the concentration of heptachlor observed in our study is not high enough to be a predicting factor for breast cancer in Ethiopia. In addition, it could be due to heptachlor and its metabolite possess a low chronic risk to humans [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Exposure to organochlorine pesticides as a predictor to breast cancer: A case-control study among Ethiopian women

et al. 2021

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Breast cancer (BC) is becoming one of the most prevalent non-infectious disease in low and middle income countries. The steady rise of BC incidence may be related to the different risk factors. Among many, rampant presence of environmental pollutants might be one of the risk factors. Therefore, the aim of this study is to investigate exposure to organochlorine pesticides as a risk factor to breast cancer. A case-control study design was employed among breast cancer patients and non-breast cancer individuals (controls). Blood samples were collected from 100 study participants (50 cases and 50 controls) followed by serum separation, extraction and cleanup using standard analytical procdures. The findings revealed that ten organochlorine pesticides were detected in the serum of the study participants. From the detected organochlorine pesticides, heptachlor was observed at higher concentration for breast cancer patients (6.90±4.37 μg/L) and controls (9.15±3.84 μg/L). Mean serum level of p,p’-DDE, p,p’-DDT, heptachlor, gamma-chlordane, endosulfan, and dibutyl-chlorendate were significantly higher in the serum of breast cancer patients than the controls. From the studied pesticides, p,p’-DDT and gamma-chlordane are significant predictors for BC, while, others are equivocal. A unit increment of the concentration of p,p’-DDT (AOR; 2.03, 95% CI: 1.041–3.969) increased the odds of developing breast cancer by two, while for gamma-chlordane (AOR;3.12, 95% CI; 1.186–8.203) by three. Our study results suggesting that, organochlorines are a risk factors for breast cancer in Ethiopia. Decreasing exposure to such organochlorines might have a significant public health relevance in reducing non-communicable chronic illnesses. Besides, continues monitoring of persistent organic pollutants using body biomarkers is important for disease prevention and device mitigation measures.

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Environmental risk assessment of pesticides in Ethiopia : a case of surface water systems

Cited by 10 publications

References 71 publications

Genome‐wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with deltamethrin and DDT resistance in Anopheles arabiensis populations from Ethiopia

Genome‐wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with deltamethrin and DDT resistance in Anopheles arabiensis populations from Ethiopia

Genome-wide gene expression profiling reveals that cuticle alterations and P450 detoxification are associated with pyrethroid resistance in Anopheles arabiensis populations from Ethiopia

Exposure to organochlorine pesticides as a predictor to breast cancer: A case-control study among Ethiopian women

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