Environmental sampling for aspergilli during building construction on a hospital site

Goodley, J.M.; Clayton, Yvonne M.; Hay, R. J.

doi:10.1016/0195-6701(94)90076-0

Cited by 110 publications

(51 citation statements)

References 14 publications

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“…A. terreus is widespread in the environment, particularly in soil and on decaying vegetation [l]. It has also been found in the air of hospital areas [3,4]. Invasive infections in man and animals have been reported [5,, showing that A. terreus is not simply a colonising organism.…”

Section: Introductionmentioning

confidence: 99%

Value of different methods for the characterisation of Aspergillus terreus strains

Rath¹,

Kamphoff²,

Ansorg³

1999

Journal of Medical Microbiology

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To evaluate different methods for strain differentiation, 10 isolates of Aspergillus terreus from Germany and two epidemiologically unrelated strains were investigated. The sources of the isolates were patients with cystic fibrosis (4), immunosuppression (2), otitis externa (2), sinusitis (1) and endocarditis (1). Environmental isolates were obtained from a contaminated cell culture and from soil. The isolates did not differ in their macroscopic and microscopic morphology, in their protein patterns analysed by SDS-PAGE and in their susceptibility to amphotericin B and itraconazole. The RFLP analysis of total genomic DNA digested by EcoRI resulted in patterns that were too faint for interpretation. However, after hybridisation of the digested DNA with a short DNA probe of repetitive sequence, six different patterns were found. Based on the patterns of the randomly amplified polymorphic DNA (RAPD) with three primers, nine different genotypes were discriminate. RAPD patterns discriminated the epidemiologically unrelated reference strains (endocarditis isolate from Thailand, soil isolate from the USA) and the isolates from Germany. It is concluded that, in contrast to the phenotypic methods, the analysis of RAPD patterns is useful for strain differentiation of A. terreus.

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Section: Introductionmentioning

confidence: 99%

Value of different methods for the characterisation of Aspergillus terreus strains

Rath¹,

Kamphoff²,

Ansorg³

1999

Journal of Medical Microbiology

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“…Building activities have been shown to increase the concentration of aspergillus conidiae in the air with subsequent development of IA [22,24,25]. Although this conjunction is still under discussion [21,26,27]. The use of high efficiency particulate air (HEPA) filtration units with laminar air flow could markedly reduce the amount of contamination with aspergillus conidiae and the subsequent development of IA [24,28].…”

Section: Epidemiology and Risk Factorsmentioning

confidence: 99%

Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients

et al. 2001

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Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients. F. Reichenberger, J.M. Habicht, A. Gratwohl, M. Tamm. #ERS Journals Ltd 2002. ABSTRACT: Invasive pulmonary aspergillosis is a major cause of morbidity and mortality in neutropenic patients.Microbiological and serological tests are of limited value. The diagnosis should be considered in neutropenic patients with fever not responding to antibiotics, and typical findings on thoracic computed tomography scan. Whenever possible, diagnosis should be confirmed by tissue examination. Newer techniques, such as polymerase chain reaction may change the current diagnostic approach.Therapeutic strategies consist of prophylaxis in risk groups and the early application of antifungal agents in suspected or probable disease. Amphotericin B as desoxycholate or lipid formulation is the current standard medication in invasive infection, although it has major side effects. Its role is challenged by the new azole derivates, such as itraconazole and voriconazole, and the new echinocandins. Additional therapies with cytokines, such as granulocyte macrophage colony stimulating factor and interferon-c, and with granulocyte transfusions are under evaluation. In selected cases lung resection is of proven diagnostic and therapeutic value.This paper analyses the current understanding of the pathogenesis and epidemiology of invasive aspergillosis and reviews the actual diagnostic and therapeutic strategies for invasive pulmonary aspergillosis in neutropenic patients.

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“…1,2 The main route of infection in humans is via inhalation of fungal spores. 3,4 In healthy people, this rarely results in the development of sinopulmonary infection, because the fungal conidia are rapidly cleared from the airways by the innate immune system. 5 However, certain risk factors are associated with an increased chance of developing allergic broncho-pulmonary aspergillosis, aspergilloma or invasive aspergillosis.…”

mentioning

confidence: 99%

Longitudinal, in vivo assessment of invasive pulmonary aspergillosis in mice by computed tomography and magnetic resonance imaging

Poelmans

Hillen

Vanherp

et al. 2016

Laboratory Investigation

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Invasive aspergillosis is an emerging threat to public health due to the increasing use of immune suppressive drugs and the emergence of resistance against antifungal drugs. To deal with this threat, research on experimental disease models provides insight into the pathogenesis of infections caused by susceptible and resistant Aspergillus strains and by assessing their response to antifungal drugs. However, standard techniques used to evaluate infection in a preclinical setting are severely limited by their invasive character, thereby precluding evaluation of disease extent and therapy effects in the same animal. To enable non-invasive, longitudinal monitoring of invasive pulmonary aspergillosis in mice, we optimized computed tomography (CT) and magnetic resonance imaging (MRI) techniques for daily follow-up of neutropenic BALB/c mice intranasally infected with A. fumigatus spores. Based on the images, lung parameters (signal intensity, lung tissue volume and total lung volume) were quantified to obtain objective information on disease onset, progression and extent for each animal individually. Fungal lung lesions present in infected animals were successfully visualized and quantified by both CT and MRI. By using an advanced MR pulse sequence with ultrashort echo times, pathological changes within the infected lung became visually and quantitatively detectable at earlier disease stages, thereby providing valuable information on disease onset and progression with high sensitivity. In conclusion, these noninvasive imaging techniques prove to be valuable tools for the longitudinal evaluation of dynamic disease-related changes and differences in disease severity in individual animals that might be readily applied for rapid and cost-efficient drug screening in preclinical models in vivo.

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Environmental sampling for aspergilli during building construction on a hospital site

Cited by 110 publications

References 14 publications

Value of different methods for the characterisation of Aspergillus terreus strains

Value of different methods for the characterisation of Aspergillus terreus strains

Diagnosis and treatment of invasive pulmonary aspergillosis in neutropenic patients

Longitudinal, in vivo assessment of invasive pulmonary aspergillosis in mice by computed tomography and magnetic resonance imaging

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