Environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters

Aw, Tiong Gim; Gin, Karina Yew‐Hoong

doi:10.1111/j.1365-2672.2010.04701.x

Cited by 102 publications

(67 citation statements)

References 59 publications

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“…The most frequent somatic coliphage values in secondary effluents therefore normally range from 10 3 to 10 5 PFU per 100 mL and those of F-specific and RNA F-specific phages from 10 2 to 5ˆ10 4 PFU per 100 mL [20,48,74,76,95,98]. The relative proportions of somatic coliphages and F-specific and RNA F-specific bacteriophages are similar to those found in untreated wastewater.…”

Section: Abundance In Pollution Sourcesmentioning

confidence: 66%

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Torroella

Gutiérrez

Blanch

et al. 2016

Water

102

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Two groups of bacteriophages that infect Escherichia coli, somatic and F-specific coliphages, have been used in academia as both fecal and viral indicators for many years. Regulatory authorities in different parts of the world are beginning to consider coliphages as indicators of water quality in a range of settings. However, issues such as their potential replication in natural water environments, the cumbersome detection and enumeration methods, a lack of definition concerning which of the two groups should be included in future regulations, and the lack of a clear correlation between coliphages and human viruses and health risks in different water settings remain controversial. This review attempts to shed some light on these contentious issues. The conclusions are that: 1) supposing that they can replicate in some natural water settings, the contribution of coliphages replicated outside the gut will not affect the numbers contributed by fecal pollution and detected by strains recommended for standardized methods; 2) there are easy, fast, and cost-effective methods that can be used in routine laboratories after a little training; 3) perhaps the best option is to determine both groups in a single step; and 4) the low correlation of coliphages with human viruses and health risks is no worse than the correlation between different human viruses.

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Section: Abundance In Pollution Sourcesmentioning

confidence: 66%

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Torroella

Gutiérrez

Blanch

et al. 2016

Water

102

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“…Características do vírus da hepatite A (VHA) como a estabilidade no meio ambiente, principalmente quando associado à matéria orgânica, e a resistência ao pH baixo e ao calor favorecem a transmissão por água e alimentos contaminados e também a detecção em amostras ambientais de água e esgoto 3 . Mesmo em países considerados não endêmicos há evidências de circulação do vírus A em um número significativo de amostras de esgotos, possivelmente introduzido por viajantes 5 . Transmissão por outras vias, como a percutânea e a parenteral, também têm sido relatadas 3,6 , mas são menos frequentes devido à menor concentração de vírus no sangue 7 .…”

Section: Introductionunclassified

Shifting susceptibility to hepatitis A among children and adolescents over the past decade

Krebs¹,

Ranieri²,

Kieling³

et al. 2011

J Pediatr (Rio J)

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“…A large quantity of viral particles is excreted in the feces of infected people (6,7), and they are finally circulated through the wastewater network (8,9). Viruses are generally not eliminated by wastewater treatment plants (WWTPs), and as a consequence, they can be released into rivers at noticeable levels (10)(11)(12).…”

mentioning

confidence: 99%

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

Prévost

Lucas

Ambert-Balay³

et al. 2015

Appl Environ Microbiol

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cAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.T he epidemiology of viral gastroenteritis is mainly evaluated through the use of clinical data. However, clinical data provide only a partial vision of the gastroenteritis viruses circulating in the human population. Indeed, many viral infections do not require specialized consultations or hospitalizations and so are not directly included in clinical statistics. As a consequence, these data sets offer a limited view of the diversity and occurrence of enteric viruses in the human population.Human astroviruses (HAstVs) and noroviruses (NoVs) are among the main causes of human acute gastroenteritis worldwide (1, 2). HAstVs and NoVs are nonenveloped viruses and have a positive-sense single-stranded RNA. The genome of HAstV is composed of three open reading frames (ORFs), and ORF2, encoding the capsid protein precursor, allows discrimination of eight genotypes of HAstV, HAstV-1 to HAstV-8 (3). The genome of NoV is also composed of three ORFs, and genotyping based on the genetic similarity of ORF2 and ORF3 allows discrimination of 9 genotypes of NoV genogroup I (NoV GI) and 22 genotypes of NoV genogroup II (NoV GII), which are the most common genogroups associated with human infections.HAstVs and NoVs are able to infect i...

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Environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters

Cited by 102 publications

References 59 publications

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Coliphages as Model Organisms in the Characterization and Management of Water Resources

Shifting susceptibility to hepatitis A among children and adolescents over the past decade

Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

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