Environmentally Friendly Poly(Vinyl Alcohol)/Poly(Methyl Methacrylate) Materials with High-Performance by a Facile Fabrication Strategy

Wang, Wenqiang; Gong, Yiming; Sun, Qingqing; Xu, Airong

doi:10.1080/00222348.2022.2125673

Cited by 1 publication

(1 citation statement)

References 20 publications

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“…In comparison, the percentage of secondary protonated amines in Asahipak NH2P-50 2D at pH 9.8 is more than 93%, indicating that it has sufficient AEX properties. 11,18) Conversely, poly(glycerol dimethacrylate) particles have higher mechanical strength than poly(vinyl alcohol) packing materials, 19) so the column pressure resistance of Gelpack GL-HilicAex (pressure limit of 40 MPa) is higher than that of Asahipak NH2P-50 2D (pressure limit of 10 MPa), as shown in Table 1. Therefore, LC performance comparisons of polar metabolomic analysis using both columns were performed under optimal flow rate conditions within their respective column pressure limits (Table 1 and Fig.…”

Section: Comparison Of Stationary Phase Properties Between a Gelpack ...mentioning

confidence: 99%

Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)

Ikeda,

Takahashi,

Bamba

et al. 2024

Mass Spectrometry

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In metabolomic analysis, one of the most commonly used techniques to support the detection sensitivity and quantitation of mass spectrometry is combining it with liquid chromatography. Recently, we developed a method that enables comprehensive single-run measurement of hydrophilic metabolites using unified-hydrophilic interaction/anion exchange liquid chromatography/high-resolution mass spectrometry (unified-HILIC/AEX/HRMS) with a polymer-based mixed amines column (Gelpack GL-HilicAex). However, the importance of stationary phase functional groups and mobile phase conditions for the separation mechanisms and sensitive detection in unified-HILIC/AEX/HRMS is not yet fully understood. This study aimed to understand the importance of the mobile and stationary phases in unified-HILIC/AEX/HRMS. Two different alkali-resistant polymer-based amines-modified columns (Gelpack GL-HilicAex, primary, secondary, tertiary, and quaternary amine-modified polyglycerol dimethacrylate gel; Asahipak NH2P-50 2D, secondary amine-modified polyvinyl alcohol gel) and two eluents (acetonitrile and ammonium bicarbonate solution, pH 9.8) were used for comparative validation. A comparison of mobile phase conditions using both columns confirmed that the two-step separation from HILIC to AEX characteristic of unified-HILIC/AEX requires a linear gradient condition from acetonitrile to nearly 50% water and AEX with up to 40 mM bicarbonate ions. We found that when alkali-resistant hydrophilic polymer packing materials are modified with amines, unified-HILIC/AEX separation can be reproduced if at least one secondary amine associated with the amine series is present in the stationary phase. Furthermore, the difference in sensitivity in the HILIC and AEX modes owing to the different columns indicates the need for further improvements in the mobile phase composition and stationary phase.

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Section: Comparison Of Stationary Phase Properties Between a Gelpack ...mentioning

confidence: 99%

Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)

Ikeda,

Takahashi,

Bamba

et al. 2024

Mass Spectrometry

View full text Add to dashboard Cite

show abstract

Environmentally Friendly Poly(Vinyl Alcohol)/Poly(Methyl Methacrylate) Materials with High-Performance by a Facile Fabrication Strategy

Cited by 1 publication

References 20 publications

Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)

Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS)

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