Environmentally relevant concentrations of benzo[a]pyrene affect steroid levels and affect gonad of male scallop Chlamys farreri

Tian, Shuangmei; Pan, Luqing; Tao, Yadong; Sun, Xiaohua

doi:10.1016/j.ecoenv.2015.01.019

Cited by 13 publications

(3 citation statements)

References 37 publications

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“…17 Similar studies have been done on male scallop Chlamys farreri and found that BaP affects steroid levels and induces gonadal toxicity at ambient concentrations. 18 Overall, BaP can cause damage to the male reproductive system, and its mechanism may be related to decreased sex hormone levels and increased apoptosis of reproductive cells. In recent years, few articles have been written about the methylation caused by BaP.…”

Section: Discussionmentioning

confidence: 99%

Abnormal methylation of spermatozoa induced by benzo(a)pyrene in rats

Zhang

Sun²,

Wang

et al. 2019

Hum Exp Toxicol

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Epigenetic mutations caused by pollutants are possibly linked to many diseases. Benzo(a)pyrene (BaP) is one of the most representative air pollutants and has aroused wide concern because of its strong carcinogenicity. The reproductive toxicity induced by BaP has been identified, but little is known about the characteristics of the methylation changes induced by BaP. In this study, a methylated DNA immunoprecipitation sequencing method was used to detect the methylation of sperm DNA of rats exposed to BaP. Compared with the respective genes in normal rats, there were 3227 hypomethylated genes and 828 hypermethylated genes after BaP exposure. Gene ontology enrichment analysis reported that differentially methylated genes (DMGs) were enriched in the localization, single-multicellular organism process and plasma membrane. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the DMGs were significantly enriched in the Ras signalling pathway, Rap1 signalling pathway, pancreatic secretion and neuroactive ligand–receptor interaction. DisGeNET disease spectrum analysis showed that DMGs were associated with infertility and certain genetic diseases. Further research needs to be done to explore whether these abnormal methylation are transgenerational.

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Section: Discussionmentioning

confidence: 99%

Abnormal methylation of spermatozoa induced by benzo(a)pyrene in rats

Zhang

Sun²,

Wang

et al. 2019

Hum Exp Toxicol

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“…The retardation of oogenesis and oocytes resorption were observed in the ovary of scallop (Mizuhopecten yessoensis) from heavy organic polluted bay (Vaschenko et al 1997). Tian et al (2015) and Yang et al (2020a) found that B[a]P exposure resulted in a signi cant decrease in the number of mature sperms and oocytes, respectively. What's more, germ cell apoptotic rate has been used as a reproductive biomarker of contaminant exposure in male sh (Zoarces viviparus) (Lyons et al 2004).…”

Section: Exploration Of the Toxic Mechanism Of Oogenesis Damage Under...mentioning

confidence: 99%

Reproductive toxicity induced by benzo[a]pyrene exposure: first exploration highlighting the multi-stage molecular mechanism in female scallop Chlamys farreri

Pan

Zhou

et al. 2022

Environ Sci Pollut Res

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Reproductive toxicity induced by Benzo[a]pyrene (B[a]P) exposure have received great ecotoxicological concerns. However, huge gaps on molecular mechanism still exist in bivalves. In this study, reproductionrelated indicators during reproductive periods (proliferative, growth, mature, and spawn stage) were investigated in female scallops Chlamys farreri, which under gradient concentration of B[a]P at 0, 0.04, 0.4 and 4 μg/L. To elucidate the potential molecular mechanisms of reproductive toxicology, a multistage ovarian transcriptome analysis under 4 μg/L B[a]P exposure was also conducted. The results indicated that life-cycle exposure to 0.4 and 4 μg/L B[a]P had signi cantly decreased GSI and sex steroid levels. Even 0.04 μg/L B[a]P could play the wicked role on DNA integrity at mature and spawn stages.Ovarian histological sections showed the inhibitions on oocyte maturation and ovulation of B[a]P with dose-dependent effects. Through the functional enrichment analysis of DEGs from transcriptome data, 18 genes involved in endocrine disruption effects, DNA damage and repair, and oogenesis damage were selected and further determined by qRT-PCR. The down-regulate of steroidogenic and estrogen signaling pathways genes indicated the endocrine disruption mechanisms by B[a]P, which emphasized the functions of receptor independent and dependent pathways under B[a]P exposure. The variation of DNA single strand break and repair gene expressions implied there might exist the similar toxic mechanism with that in vertebrates. Gene expression data involved in cell cycle, apoptosis and cell adhesion exhibited the possibly toxic mechanisms of oogenesis caused by B[a]P. Taken together, this study is a pioneer to take advantage of genome-wide transcriptomic analysis and its corresponding reproductive indicators to explore the toxic mechanism under B[a]P exposure in bivalves. Meanwhile, some selected genes were rstly identi ed in bivalves, and the expression data might be useful in establishing new hypotheses and discovering new biomarkers for marine biomonitoring.

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“…The CYP1A subfamily (Figure 7) is the most studied among the cytochrome P450 family of enzymes as it is significantly induced by PAHs. The activity of this marker in fish and shellfish has been applied in many assessments to verify PAH effects, especially BaP, which significantly increases the expression of CYP1A as a function of exposure time and dose [47,59,[85][86][87][88][89]92,93,100].…”

Section: Biomarker Evaluationsmentioning

confidence: 99%

The Role of the Ecotoxicology Applied to Seafood as a Tool for Human Health Risk Assessments Concerning Polycyclic Aromatic Hydrocarbons

Pinho

Rodrigues

Guimarães

et al. 2022

IJERPH

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Background: Polycyclic aromatic hydrocarbons (PAHs) are persistent pollutants routinely detected in aquatic ecosystems. It is, therefore, necessary to assess the link between deleterious marine biota PAH effects, especially in commercialized and consumed animals, environmental health status, and potential human health risks originating from the consumption of contaminated seafood products. Thus, this review seeks to verify the relationships of ecotoxicological studies in determining effect and safety concentrations on animals routinely consumed by humans. Methods: A total of 52 published studies between 2011 and 2021, indexed in three databases, were selected following the PICO methodology, and information on test animals, evaluated PAH, and endpoints were extracted. Results: Benzo(a)pyrene and phenanthrene were the most investigated PAHs in terms of biomarkers and test organisms, and mussels were the most evaluated bioindicator species, with an emphasis on reproductive responses. Furthermore, despite the apparent correlation between environmental PAH dynamics and effects on aquatic biota and human health, few assessments have been performed in a multidisciplinary manner to evaluate these three variables together. Conclusions: The links between human and environmental sciences must be strengthened to enable complete and realistic toxicity assessments as despite the application of seafood assessments, especially to mussels, in bioassays, the connection between toxicological animal responses and risks associated with their consumption is still understudied.

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Environmentally relevant concentrations of benzo[a]pyrene affect steroid levels and affect gonad of male scallop Chlamys farreri

Cited by 13 publications

References 37 publications

Abnormal methylation of spermatozoa induced by benzo(a)pyrene in rats

Abnormal methylation of spermatozoa induced by benzo(a)pyrene in rats

Reproductive toxicity induced by benzo[a]pyrene exposure: first exploration highlighting the multi-stage molecular mechanism in female scallop Chlamys farreri

The Role of the Ecotoxicology Applied to Seafood as a Tool for Human Health Risk Assessments Concerning Polycyclic Aromatic Hydrocarbons

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