Environmentally relevant concentrations of citalopram partially inhibit feeding in the three-spine stickleback (Gasterosteus aculeatus)

Abstract

PostprintThis is the accepted version of a paper published in Aquatic Toxicology. This paper has been peerreviewed but does not include the final publisher proof-corrections or journal pagination.Citation for the original published paper (version of record):Kellner, M., Porseryd, T., Porsch-Hallstrom, I., Hansen, S., Olsén, H. (2015) Environmentally relevant concentrations of citalopram partially inhibit feeding in the three-spine stickleback (Gasterosteus aculeatus).Aquatic Toxicology,[158][159][160][161][162][163][164][165][166][167][168][169][170] http://dx.doi.org/10.1016/j.aquatox. 2014.11.003 Access to the published version may require subscription. N.B. When citing this work, cite the original published paper. NOTICE: this is the author's version of a work that was accepted for publication in Aquatic Toxicology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquatic Toxicology, Vol. 158,[165][166][167][168][169][170] Abstract Selective Serotonin Re-uptake Inhibitors (SSRI) are mood-altering, psychotropic drugs commonly used in the treatment of depression and other psychological illnesses. Many of them are poorly degraded in sewage treatment plants and enter the environment unaltered. In laboratory studies, they have been demonstrated to affect a wide range of behaviours in aquatic organisms. In this study we investigated the effect of a three week exposure of 0.15 and 1.5 µg/l of the SSRI citalopram dissolved in the ambient water on the feeding behaviour in three-spine stickleback (Gasterosteus aculeatus). Feeding, measured as the number of attacks performed on a piece of frozen bloodworms during a 10 minute period, was reduced by 40-60% in fish exposed to both 0.15 and 1.5 µg/l citalopram. The effects of the environmentally relevant dose 0.15 µg/l on feeding, an important fitness characteristic, suggests that the ecological significance of environmental SSRI exposure may be pronounced.

Impact of the antidepressant citalopram on the behaviour of two different life stages of brown trout

“…In our experiment, we visually observed more food leftovers in the exposure tanks with 1,000 µg/L citalopram, which allowed us to conclude that there was a lower food intake in fish exposed to 1,000 µg/L citalopram; however, a quantification of this effect was not possible. Known side effects of citalopram are anorexia and weight loss in humans (Information of HEXAL (2012)) and published data on fish revealed a decreased food intake in sticklebacks exposed to 0.15 µg/L citalopram (Kellner et al, 2015). Reduced food intake in fish was also shown for two other antidepressants, sertraline and fluoxetine, in European perch and goldfish (Hedgespeth, Nilsson & Berglund, 2014;Mennigen et al, 2010).…”

“…But, Olsen et al (2014) could show anxiolytic effects in the novel tank diving test, like reduced freezing behaviour and faster and longer time spent in the upper aquaria in Endler's guppies exposed to 2.3 and 15 µg/L of citalopram for 21 days. With regard to feeding behaviour, Kellner et al (2015) showed that three-spined sticklebacks had reduced food intake when exposed to 0.15 µg/L citalopram for 21 days. But in contrast, Kellner et al (2017) observed increased food intake in sticklebacks exposed to 1.5 µg/L citalopram for 30 days during developmental stages, and a subsequent 120 days recovery phase.…”

The Psychoactive Drug Escitalopram Affects Foraging Behavior in Zebrafish (Danio rerio)

“…Swimming behavior is a widely used endpoint of chemical impacts on fish (e.g., Kellner et al 2015;Storgaard et al 2017;Nielsen et al 2018). Kalueff et al (2013) provide a comprehensive catalog of zebrafish behaviors.…”

“…The SERTs are comparable across different vertebrate taxa, and the 5-HT system is remarkably conserved in animal evolution (Wang et al 2006;Lillesaar 2011). Among a wide array of physiological states, functions, and behaviors (Kreke and Dietrich 2008;Lillesaar 2011;Gaspar and Lillesaar 2012;Maximino et al 2013;Prasad et al 2015;Winberg and Thörnqvist 2016), serotonergic activity has been implicated in the control of food intake in fish (Matsuda 2009;Kellner et al 2015). Previous fish studies have demonstrated that the SSRI fluoxetine can regulate neuropeptides as well as metabolic parameters and related enzymes controlling energy balance (Mennigen et al 2009(Mennigen et al , 2010.…”

Can the behaviour of threespine stickleback parasitized with Schistocephalus solidus be replicated by manipulating host physiology?

“…Feeding latency Giles, 1983, 1987Kellner et al, 2015Amodeo et al, 2015Brodin et al, 2013Croy and Hughes, 1991Damsgird andDill, 1998 Henry et al, 2008 INF, sticklebacks infected with S. solidus; FLX, fluoxetine; OXA, oxazepam; STRV, fasting; LPS, lipopolysaccharide. The '?'…”