Environmentally relevant concentrations of triclocarban affect behaviour, learning, and brain gene expression in fish

Lucon-Xiccato, Tyrone; Savaşçı, Beste Başak; Merola, Carmine; Benedetti, Elisabetta; Caioni, Giulia; Aliko, Valbona; Bertolucci, Cristiano; Perugini, Monia

doi:10.1016/j.scitotenv.2023.166717

Cited by 9 publications

(3 citation statements)

References 101 publications

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“…It is involved in immune responses, in ammation, wound healing, bone development, and neuronal plasticity [18,19]. Fos protein has also been linked to several diseases, including cardiovascular diseases, neurodegenerative disorders, and metabolic disorders [20].…”

Section: Discussionmentioning

confidence: 99%

Functional investigation and two-sample Mendelian randomization study of non-alcoholic fatty liver disease hub genes obtained by WGCNA

Yang,

Ma,

Zhou

et al. 2023

Preprint

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Objective: Non-alcoholic fatty liver disease (NAFLD) poses significant health risks, including the potential progression to more severe liver conditions such as liver fibrosis, cirrhosis, and even hepatocellular carcinoma, but its underlying mechanisms are not well understood. This study aimed to identify potential hub genes for NAFLD and evaluate their clinical application in predicting the condition. Methods: We conducted differential expression analysis and weighted gene co-expression network analysis (WGCNA) to identify NAFLD susceptibility modules and hub genes. We performed KEGG and GO analyses to explore the potential roles of these hub genes. We developed a nomogram model and ROC curves to assess the diagnostic efficacy of the hub genes. Additionally, we investigated the correlation between FOS and immune infiltration. Finally, we conducted a Mendelian randomization study based on genome-wide association studies to determine the causal effect of FOS on NAFLD. Results: WGCNA analysis was conducted to construct gene co-expression networks, identify the most significant module, and identify 115 key genes derived from the overlapping results of WGCNA and differential expression analysis. GO and KEGG pathway enrichment analyses revealed that these key genes were associated with fat cell differentiation, ameboidal−type cell migration, response to lipopolysaccharide, TNF signaling pathway, MAPK signaling pathway, and AGE−RAGE signaling pathway in diabetic complications. Using Cytoscape software, we identified the top ten up-regulated genes with high scores: FOS, JUN, NR4A1, JUNB, EGR1, MYC, IL1B, CCL2, CXCL8, and PTGS2. Furthermore, our nomogram model demonstrated good performance in predicting NAFLD, and the ROC curve confirmed its diagnostic effectiveness. Finally, we focused on FOS and observed a causal association between FOS and immune cell infiltrates in NAFLD. In the inverse variance weighting analysis, we found that FOS was not associated with the risk of NAFLD, with an odds ratio of 0.997 (95% CI = 0.947-1.049, p = 0.898). Conclusion: We identified hub genes related to NAFLD, which may provide insights into early diagnostic approaches and contribute to the understanding of molecular mechanisms underlying NAFLD risk genes.

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Section: Discussionmentioning

confidence: 99%

Functional investigation and two-sample Mendelian randomization study of non-alcoholic fatty liver disease hub genes obtained by WGCNA

Yang,

Ma,

Zhou

et al. 2023

Preprint

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show abstract

“…This test allows the earliest cognitive assessment in zebrafish. It has been shown to be sensitive to alterations in learning owing to pollutants even at 4 dpf [38], and can predict cognitive abilities of older larvae (electronic supplementary material, S1). The assay consisted of administering 25 additional stimulations following the stimulation for the startle test.…”

Section: (C) Habituation Learning Testmentioning

confidence: 99%

“…The stimulations were always separated by a 1 s interval. Using the distance moved by each subject after each stimulation, we calculated an index of activity reduction between each subsequent stimulation and the first [38,39]. This index provided a measure of habituation learning as the reduction in each individual's response to the repeated stimulation.…”

Section: (C) Habituation Learning Testmentioning

confidence: 99%

Embryonic exposure to artificial light at night impairs learning abilities and their covariance with behavioural traits in teleost fish

Lucon-Xiccato,

De Russi,

Cannicci

et al. 2023

Biol. Lett.

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The natural light cycle has profound effects on animals' cognitive systems. Its alteration owing to human activities, such as artificial light at night (ALAN), affects the biodiversity of mammalian and avian species by impairing their cognitive functions. The impact of ALAN on cognition, however, has not been investigated in aquatic species, in spite of the common occurrence of this pollution along water bodies. We exposed eggs of a teleost fish (the zebrafish Danio rerio ) to ALAN and, upon hatching, we measured larvae’ cognitive abilities with a habituation learning paradigm. Both control and ALAN-exposed larvae showed habituation learning, but the latter learned significantly slower, suggesting that under ALAN conditions, fish require many more events to acquire ecologically relevant information. We also found that individuals' learning performance significantly covaried with two behavioural traits in the control zebrafish, but ALAN disrupted one of these relationships. Additionally, ALAN resulted in an average increase in larval activity. Our results showed that both fish's cognitive abilities and related individual differences are negatively impacted by light pollution, even after a short exposure in the embryonic stage.

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Differential impact of artificial light at night on cognitive flexibility in visual and spatial reversal learning tasks

De Russi,

Montalbano,

Gatto

et al. 2024

Animal Behaviour

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Environmentally relevant concentrations of triclocarban affect behaviour, learning, and brain gene expression in fish

Cited by 9 publications

References 101 publications

Functional investigation and two-sample Mendelian randomization study of non-alcoholic fatty liver disease hub genes obtained by WGCNA

Functional investigation and two-sample Mendelian randomization study of non-alcoholic fatty liver disease hub genes obtained by WGCNA

Embryonic exposure to artificial light at night impairs learning abilities and their covariance with behavioural traits in teleost fish

Differential impact of artificial light at night on cognitive flexibility in visual and spatial reversal learning tasks

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