Environmentally sensitive nanohydrogels decorated with a three-strand oligonucleotide helix for controlled loading and prolonged release of intercalators

Abstract

Biocompatible nanohydrogels modified with three-segment oligonucleotide hybrids were used for controlled loading and prolonged release of anticancer intercalators in hyperthermia treatment.

Switchable conformational changes of DNA nanogel shells containing disulfide–DNA hybrids for controlled drug release and efficient anticancer action

“…It should be stressed here, that Dox uorescence is dramatically quenched upon its intercalation into dsDNA and the number of occupied DNA base pairs per one Dox molecule can vary depending on preferred mode of its interaction. 31 As it was expected, the uorescence intensity was lower for Dox accumulated in PNIPA-AAc-PEG-SS-dsDNA NGs. The decrease in Dox incubation in insulinoma cells might be also the effect of the increased detoxication capacity of the drug resistance mechanisms.…”

“…27 Thanks to the presence of DNA crosslinkers in the so nanogel network it was possible to get modulation of reversible phase transition of the hydrogel lattices for the achievement of selective drug binding and prolonged and controlled release of drugs. 31,32 Herein, we report on the development of a biocompatible, multi-responsive DNA nanogel containing specic disulde oligonucleotide-based co-crosslinkers for highly efficient loading and triggered, switchable drug release. Under physiological conditions the novel nanogel existed as a core-shell structure; it contained a polymeric core and an oligonucleotidebased shell.…”

Recent Advances in Degradable Hybrids of Biomolecules and NGs for Targeted Delivery

“…As it was mentioned, the size of NGs, the duration time of the nanogel collapse effect, and the versatility of volume phase transition are strongly size-dependent, see Figure 4 [49]. In general, polymers and monomers usually applied for the design of hybrid NGs for biomedical application (e.g., poly( N -isopropylacrylamide, PNIPA) possess a volume phase transition temperature (VPTT) lower than that of the physiological use; it is in the range of 31–34 °C [50,51].…”

“…Biomolecules accumulated/grafted/bioconjugated to the nanogel network can move the VPTT to higher temperatures [50,51,52]. Despite the promising features of biomolecule crosslinkers, only a few examples were presented in the literature for the design of thermoresponsive hybrid NGs, perhaps because of the difficulty of its conjugation with nanogel surfaces.…”

“…Despite the promising features of biomolecule crosslinkers, only a few examples were presented in the literature for the design of thermoresponsive hybrid NGs, perhaps because of the difficulty of its conjugation with nanogel surfaces. Liwinska et al [50,51] proposed several approaches for covalent binding of oligonucleotides as the crosslinkers in temperature- and pH-responsive hybrid NGs. The introduction of programmed specific tri-segment structures of oligonucleotides resulted in an increase in VPTT to 37 °C, see Figure 5A(I).…”