Changes in soil biochemical and cracking properties induced by "living mulch" systems. Can. J. Soil Sci. 77: 579-587. A vermicompost (VC) from biological sludges was incorporated into two soils, or applied on their surfaces as mulch with the addition of worms (Eisenia foetida) or grass seeds (Lepidium sativum) in the experiments of "living mulch". The purpose of this research was to study the influences of different methods of organic matter application on soil chemico-physical and biochemical properties. Two texturally different soils from a Mediterranean climate, a clayey and a sandy soil, were tested in laboratory-scale experiments. Water-soluble C (WSC), NO 3 -N/NH 4 -N ratio (nitrification index), and enzyme activities (BAA-protease, dehydrogenase, and β-glucosidase) were taken as indices of soil biochemical fertility as they are related to soil physical properties and microbial activities. Surface cracking area in both soils increased, with the formation of small-medium (<1000 µm) cracks. The positive changes in cracking, the high index of nitrification, and the evolution of WSC provided evidence for high metabolism in mulched soils. Enzyme activity was similar in living mulch and control soils, and was highest when VC was directly incorporated. Significant correlations (P < 0.05) occurred between enzyme activities, organic substrates, and soil cracking area for both soils. The selected parameters were indicators of the different practices of organic matter application.