Enzymatic activity in braúna seeds subjected to thermal stress

Ataíde, Glauciana da Mata; Borges, Eduardo Euclydes de Lima e; Flores, Andressa Vasconcelos

doi:10.1590/0103-8478cr20141800

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…In seeds exposed to heat stress, glucose levels decreased been accompanied by a reduction in α and β-amylase enzyme activities. In M. brauna, heat stress also reduced the activity of α and β-amylase after a period of 48 h of imbibition (Ataíde et al, 2016). All these data show that the supply of sugars was affected after forest seeds (O. coarctata or M. brauna) to suffer heat stress.…”

Section: Discussionmentioning

confidence: 78%

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Effects of high temperature on morphophysiological, ultrastructural, and biochemical parameters during the germination of two forest species

Reis¹

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Climate change resulting from anthropogenic actions has increased global temperature. These temperature increases will likely influence the survival of part of the species. Among the environmental factors, the temperature is one of the most critical for affecting the physiological, biochemical, and molecular factors of the seed. Temperature increases is considered one of the environmental factors that help in the overproduction of reactive oxygen species (ROS). The urgency of understanding seed responses to climate change is particularly important. A detailed overview of seed responses to heat stress can help formulate appropriate strategies for species conservation and preservation. Knowledge about the capacity of forest seeds to deal with temperature changes is still scarce. In this sense, the objectives of this work were: 1. To investigate the morphophysiological, biochemical, and ultrastructural changes during germination of Melanoxylon brauna seeds under heat stress; 2. Evaluate the effects of temperature on morphology, generation of ROS, antioxidant system, and mobilization of reserves in Ormosia coarctata seeds; 3. Characterize the effects of diphenyleneiodonium (DPI) on M. brauna germination, internal anatomy, hydrogen peroxide (H 2 O 2 ) content, and activity of reserve enzymes and antioxidant enzymes under optimal conditions (25 °C) and stress by heat (40°C). To evaluate the internal morphology the seeds were radiographed. Ultrastructural and anatomical parameters were evaluated using transmission electron microscopy and light microscopy. ROS production, malondialdehyde (MDA) and glucose content, carbonyl proteins, and enzyme activity (superoxide dismutase - SOD, ascorbate peroxidase - APX, catalase - CAT, peroxidase - POX, glucose-6-phosphate dehydrogenase - G6PDH, lipase, α- and β-amylase and protease) were measured by spectrophotometric analysis. The high temperature causes a reduction in the percentage and speed of germination and affects the internal morphology of M. brauna and O. coarctcta seeds. In M. brauna seeds, heat stress decreases respiratory rates and compromises the structure of mitochondria. ROS content and protein carbonylation in seeds submitted to 40 °C increased in relation to 25 °C and 35°C. The activities of the enzymes SOD, APX, CAT, and POX were significantly reduced in seeds subjected to heat stress. Glucose content, G6PDH (M. brauna), and lipase activity also decreased in seeds exposed to high- temperature stress. Seed exposure to DPI decreased germination percentage at 25 °C and does not affect germination at 40 °C. DPI caused less reduction in the loosening of the outer layers of the integument and reduced the H 2 O 2 content and activity of all tested enzymes. In summary, heat stress negatively affects germination, promotes oxidative stress, and induces damage to mitochondrial ultrastructure, seed morphology, and anatomy. The data obtained in this study contribute to a better understanding of the effects of high temperatures on the germination of native seeds, in addition to showing the contribution of ROS to germination. Keywords: Climate change. Heat stress. Melanoxylon brauna. Ormosia coarctata.

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Section: Discussionmentioning

confidence: 78%

“…Studies have also shown that high temperature stress affects the activity of storage enzymes. In seeds of different species such as M.brauna, Erythrina velutina, and O. coarctata, the mobilization of reserves is impaired after heat stress (Ataíde et al, 2016;Felix et al, 2020;.…”

Section: Introductionmentioning

confidence: 99%

Effects of high temperature on morphophysiological, ultrastructural, and biochemical parameters during the germination of two forest species

Reis¹

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Seed germination in Tephrosia egregia Sandwith (Fabaceae), a species native to the brazilian Caatinga ecoregion with potential for recovery of degraded areas

Moraes,

Ribeiro,

Silva

et al. 2023

Hoehnea

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Tephrosia egregia Sandwith is a species that develops in some States of the Northeast region of Brazil. It is propagated by seeds and has considerable potential for restoration. Thus, studies related to seed germination are important for the species. The aims were to characterize the post-seminal development and evaluate the germination response of the seeds under different temperatures and water stress and salt stress. Germination tests were performed at 20, 25, 30 and 20-30 °C and under PEG 6000 and NaCl concentrations (-0.2, -0.4, -0.6 and -0.8 MPa). The germination test should be conducted in a paper roll at 25 ºC for 14 days. Seed germination declines from the water potential of -0.2 MPa on; the species is sensitive to water stress. Under salt stress, there is more accentuated decline in germination as from -0.4 MPa, and this is an indication have a certain tolerance to salinity.

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PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

Flores

Ataíde

Borges³

et al. 2018

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In order to ensure the ex-situ conservation of a species, knowing the behavior of the seed during storage is necessary, as it is influenced by several factors inherent to the seed and by characteristics of the environment, such as temperature and relative humidity. This study aimed to evaluate the physiological and biochemical alterations on Cedrela fissilis (pink cedar) seeds during storage under different environmental conditions. Seeds were stored at 20ºC in desiccators containing salts that established the relative humidity of 34 %, 55 %, 75 %, and 93 %. Analysis were performed on fresh seeds (without storage -time zero), which were used as control, and every two months through a period of eight months of storage. At each assessment, the following aspects were quantified: water content; lipid peroxidation; and the activities of catalase and glucose-6-phosphate dehydrogenase. Germination and electrical conductivity tests were analysed. Seed vigor decreased on humidity at 20 °C. It correlated mainly with decrease of glucose-6-phosphate dehydrogenase activity and increase of lipid peroxidation. Keywords: Pink cedar, germination, vigor, enzymes, lipids. ResumoAlterações fisiológicas e bioquímicas durante o armazenamento de sementes de Cedrela fissilis Vellozo. Para garantir a conservação ex-situ de uma espécie, é necessário conhecer o comportamento das sementes durante o armazenamento, visto que este é influenciado por vários fatores inerentes à semente e às características do ambiente, tais como temperatura e umidade relativa. Este estudo teve como objetivo avaliar as alterações fisiológicas e bioquímicas em sementes de Cedrela fissilis (cedro rosa) durante o armazenamento sob diferentes condições ambientais. As sementes foram armazenadas a 20ºC em dessecadores contendo sais que estabeleceram as umidades relativas do ar de 34 %, 55 %, 75 % e 93 %. As análises foram realizadas em sementes frescas (sem armazenamento -tempo zero), usadas como controle, e a cada dois meses durante um período de oito meses de armazenamento. Em cada avaliação, foram quantificados: teor de água; peroxidação de lípidos; e as atividades das enzimas catalase e glicose-6-fosfato desidrogenase. Também, foi analisada a germinação e condutividade elétrica das sementes. O vigor das sementes diminuiu em todas as umidades a 20 °C, correlacionando-se, principalmente, com a diminuição na atividade de glicose-6-fosfato desidrogenase e com o aumento na peroxidação lipídica. Palavras-chave: Cedro rosa, germinação, vigor, enzimas, lipídios.

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Enzymatic activity in braúna seeds subjected to thermal stress

Abstract: To occur seed germination distinct hydrolytic enzymes work together, enabling primary root protrusion. The objective of this study was to investigate changes in the activities Palavras-chave:α-amilase, β-amilase, glicose-6-fosfato desidrogenase, Melanoxylon braúna.

Cited by 3 publications

References 21 publications

Effects of high temperature on morphophysiological, ultrastructural, and biochemical parameters during the germination of two forest species

Effects of high temperature on morphophysiological, ultrastructural, and biochemical parameters during the germination of two forest species

Seed germination in Tephrosia egregia Sandwith (Fabaceae), a species native to the brazilian Caatinga ecoregion with potential for recovery of degraded areas

PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

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