Enzymatic activity monitoring through dynamic nuclear polarization in Earth magnetic field

Parzy, Elodie; Boudries, Dahmane; Jacoutot, Samuel; Albalat, Muriel; Vanthuyne, Nicolas; Jm, Franconi; Mellet, Philippe; Thiaudière, Eric; Audran, Gérard; Marque, Sylvain R. A.; Massot, Philippe

doi:10.1016/j.jmr.2021.107095

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…Moreover, at this EPR frequency, the occurrence of tissue heating could prevent the translation of this approach to larger animals or humans. In order to overcome this problem, it was recently published by Parzy and coworkers 49 , 50 a new OMRI system working at earth field, allowing a significant reduction in the value of the used EPR frequency. With this system OMRI was performed in living rats through in situ Dynamic Nuclear Polarization at 206 µT using stable and non-toxic nitroxides.…”

Section: Discussionmentioning

confidence: 99%

4-Amino-TEMPO loaded liposomes as sensitive EPR and OMRI probes for the detection of phospholipase A2 activity

Alberti,

Thiaudiere,

Parzy

et al. 2023

Sci Rep

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This work aims at developing a diagnostic method based on Electron Paramagnetic Resonance (EPR) measurements of stable nitroxide radicals released from “EPR silent” liposomes. The liposome destabilisation and consequent radical release is enzymatically triggered by the action of phospholipase A2 (PLA2) present in the biological sample of interest. PLA2 are involved in a broad range of processes, and changes in their activity may be considered as a unique valuable biomarker for early diagnoses. The minimum amount of PLA2 measured “in vitro” was 0.09 U/mL. Moreover, the liposomes were successfully used to perform Overhauser-enhanced Magnetic Resonance Imaging (OMRI) in vitro at 0.2 T. The amount of radicals released by PLA2 driven liposome destabilization was sufficient to generate a well detectable contrast enhancement in the corresponding OMRI image.

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Section: Discussionmentioning

confidence: 99%