Enzymatic activity of fungi endophytic on five medicinal plant species of the pristine sacred forests of Meghalaya, India

Bhagobaty, Ranjan Kumar; Joshi, S. R.

doi:10.1007/s12257-011-0453-4

Cited by 37 publications

(19 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sizes of the areas of substrate hydrolysis were observed to be in the range 4-13 mm for the cellulolytic activity as shown in Figure 2a and Table 1, and 1-12 mm for the xylanolytic activity as shown in Figure 2b and Table 1, something which is not very much different from what was previously exhibited by plant endophytes with a massive ability to degrade cellulose and hemicellulose that generally had diameters in the range of ≥20 mm (Tasia and Melliawati, 2017). Thus, the abilities of fungi to degrade cellulose and hemicellulose may be gained as a result of adaptation of these microbes to their habitats, which is a set of lignocellulosic materials (Bhagobaty and Joshi, 2012). At this point and irrespective of whether an isolate could hydrolyze the provided substrates or not, all isolates (10) that had shown the capability to grow on CMC and/or xylan as shown in Table 1 were further assessed for their ability to secrete cellulases and xylanases through spectrophotometry (a more sensitive technique than the agar plate assay).…”

Section: Most Studies Nowadays Have Focused On Cellulases Andmentioning

confidence: 99%

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Sibanda¹,

Ruzvidzo²,

Ncube³

et al. 2019

J. Yeast Fungal Res.

View full text Add to dashboard Cite

Cellulases and xylanases are enzymes of industrial significance, particularly in the pulp, paper, textile, and animal feed industries. Moreover, their utilization in the food industry, among them, bakery, brewery, winery and fruit and vegetable juice production, cannot be underestimated. One of the potential sources of enzymes is the filamentous fungi, and hence bio-prospecting of this specific group of microorganisms with the highest levels of cellulase and xylanase secretions is being continuously undertaken. The specific aim of this study was to isolate and characterize cellulase-and xylanaseproducing filamentous fungi from termite mounds. Termite mounds have long been established as very good sources of filamentous fungi with the ability to secrete high levels of lignocellulolytic enzymes, and hence an ideal target for the bio-prospecting of cellulases and xylanases. In this study, various groups of filamentous fungi were isolated through enrichment and repeated sub-culturing. This was followed by screening using the Congo red plate-based assay. Cellulase and xylanase activities during the solid-state fermentation of wheat bran were detected and analyzed through spectrophotometry via the 3,5-dinitrosalicylic acid detection system for reducing sugars. The obtained fungal isolates were then finally characterized through zymography, reaction kinetics and morphological studies. Overall, a total of eight different groups of fungi, capable of decomposing cellulose and hemicellulose, were isolated, and their tentative identities established as Fusarium, Didymostible, Penicillium, Phytophthora, Oedocephalum, Aspergillus, Monosporascus and Acremonium. Taken together, findings of this study conceivably showed that termite mounds are a good source of filamentous fungi that in turn are also a good source of cellulases and xylanases that arguably, can be recommended for use in industrial and commercial settings.

show abstract

Section: Most Studies Nowadays Have Focused On Cellulases Andmentioning

confidence: 99%

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Sibanda¹,

Ruzvidzo²,

Ncube³

et al. 2019

J. Yeast Fungal Res.

View full text Add to dashboard Cite

show abstract

“…Any article was identified using the Google Scholar platform. Therefore, supported by the inclusion and exclusion criteria, a review of the full texts was completed, and 15 articles were selected for this review [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. This process led to the exclusion of 55 papers (Appendix A).…”

Section: Study Selectionmentioning

confidence: 99%

“…The selected articles were carried out in eight different countries. One each from Tunisia [20], Malaysia [30], Australia [33], and the United States [25]; two from Egypt [22,34], Brazil [23,26], and China [24,32]; and five from India [21,[27][28][29]31]. The articles were published between 1994 and 2021 and were written in English.…”

Section: Study Characteristicsmentioning

confidence: 99%

Protease Produced by Endophytic Fungi: A Systematic Review

et al. 2021

View full text Add to dashboard Cite

The purpose of this systematic review was to identify the available literature of production, purification, and characterization of proteases by endophytic fungi. There are few complete studies that entirely exhibit the production, characterization, and purification of proteases from endophytic fungi. This study followed the PRISMA, and the search was conducted on five databases: PubMed, PMC, Science Direct, Scopus Articles, and Web of Science up until 18 May 2021, with no time or language restrictions. The methodology of the selected studies was evaluated using GRADE. Protease production, optimization, purification, and characterization were the main evaluated outcomes. Of the 5540 initially gathered studies, 15 met the inclusion criteria after a two-step selection process. Only two studies optimized the protease production using statistical design and two reported enzyme purification and characterization. The genus Penicillium and Aspergillus were the most cited among the eleven different genera of endophytic fungi evaluated in the selected articles. Six studies proved the ability of some endophytic fungi to produce fibrinolytic proteases, demonstrating that endophytic fungi can be exploited for the further production of agents used in thrombolytic therapy. However, further characterization and physicochemical studies are required to evaluate the real potential of endophytic fungi as sources of industrial enzymes.

show abstract

“…Osbeckia stellata Buchanan-Hamilton ex Kew Gawler is one of the most widely distributed species in this genus, ranging from southern and southwestern China to southeastern Himalayan region (Wu 2006;Das and Anjeza 2013). Although wildly growing in the field, it is a kind of endophytic fungal medicinal plant that has great potential for clinical use (Bhagobaty and Joshi 2012). It was documented that the Apatani tribe, which was located in northeastern India traditionally made use of the leaves of O. stellata to treat toothache (Kala 2005) while in Nepal, it was used to heal the wound (Balami 2004).…”

Section: Osbeckia Stellata;mentioning

confidence: 99%

Characterization of the chloroplast genome of Osbeckia stellata (Melastomataceae)

Liang

Ruan

Zhi

et al. 2019

Mitochondrial DNA Part B

View full text Add to dashboard Cite

Osbeckia stellata (Melastomataceae) is a shrub distributed in the South. Although many species of Osbeckia have important medicinal value, the phylogenetic position of Osbeckia within the family Melastomataceae remains unclear. Osbeckia stellata is one of the most widespread species in this genus. Here, we reported and characterized the complete chloroplast genome sequence of O. stellata assembled from Illumina sequencing reads. The chloroplast genome size is 156,372 bp, containing a large single copy (LSC) region of 85,741 bp, a short single copy (SSC) region of 17,115 bp, and a pair of inverted repeats (IRs) of 26,758 bp. We predicted a total of 136 genes consisting of 86 protein-coding genes, 48 tRNA genes, and 8 rRNA genes. Phylogenetic analysis showed that O. stellata was sister to Melastoma candidum.

show abstract

Enzymatic activity of fungi endophytic on five medicinal plant species of the pristine sacred forests of Meghalaya, India

Cited by 37 publications

References 16 publications

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Diversity of cellulase- and xylanase-producing filamentous fungi from termite mounds

Protease Produced by Endophytic Fungi: A Systematic Review

Characterization of the chloroplast genome of Osbeckia stellata (Melastomataceae)

Contact Info

Product

Resources

About