Enzymatic activity of Lactobacillus reuteri grown in a sweet potato based medium with the addition of metal ions

Abstract

The effect of metal ions on the enzymatic activity of Lactobacillus reuteri was studied. The enzymatic activity was determined spectrophotometrically using the corresponding substrate. In the control group, L. reuteri MF14-C, MM2-3, SD2112, and DSM20016 produced the highest α-glucosidase (40.06 ± 2.80 Glu U/mL), β-glucosidase (17.82 ± 1.45 Glu U/mL), acid phosphatase (20.55 ± 0.74 Ph U/mL), and phytase (0.90 ± 0.05 Ph U/mL) respectively. The addition of Mg2+ and Mn2+ led to enhance α-glucosidase produced by L. reuteri MM2-3 by 113.6% and 100.6% respectively. α-Glucosidase produced by MF14-C and CF2-7F was decrease in the presence of K+ by 65.8 and 69.4% respectively. β-Glucosidase activity of MM7 and SD2112 increased in the presence of Ca2+ (by 121.8 and 129.8%) and Fe2+ (by 143.9 and 126.7%) respectively. Acid phosphatase produced by L. reuteri CF2-7F and MM2-3 was enhanced in the presence of Mg2+, Ca2+ or Mn2+ by (94.7, 43.2, and 70.1%) and (63.1, 67.8, and 45.6%) respectively. On the other hand, Fe2+, K+, and Na+ caused only slight increase or decrease in acid phosphatase activity. Phytase produced by L. reuteri MM2-3 was increase in the presence of Mg2+ and Mn2+ by 51.0 and 74.5% respectively. Ca2+ enhanced phytase activity of MM2-3 and DSM20016 by 27.5 and 28.9% respectively. The addition of Na+ or Fe2+ decreased phytase activity of L. reuteri. On average, Mg2+ and Mn2+ followed by Ca2+ led to the highest enhancement of the tested enzymes. However, the effect of each metal ion on the enzymatic activity of L. reuteri was found to be a strain dependent. Therefore, a maximized level of a target enzyme could be achieved by selecting a combination of specific strain and specific metal ion.

Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers

“…α‐Glucosidases activity towards p ‐nitrophenyl‐α‐ d ‐glucopyranoside (pNPG) was measured with use of amount of p ‐nitrophenol (pNP) liberated from pNPG using a calibration curve at 420 nm (Hayek, Shahbazi, Worku, & Ibrahim, ). 10 m m pNPG as a substrate in sodium phosphate buffer was added to the mixture to start the reaction.…”

Effects of calcium level and source, acidification, and phytase on phytate degradation and the microbiota in the digestive tract of broiler chickens

“…Relative abundance of OTUs assigned to strains of L. reuteri summed up to a range of 6-26%. At the low Ca level, the relative abundance of some OTUs assigned to L. reuteri (OTU4, OTU9, OTU11, and OTU13) were less abundant in the crop, which corroborates the nding that Ca can inhibit the growth of L. reuteri [15]. Hayek et al [15] found that phosphatase activity (probably including phytase) produced by L. reuteri depended on strain and presence of chemical elements in a nutrient solution.…”

“…Functional prediction and changes in the relative abundance of some bacteria point towards an in uence of the microbial community on P utilization. Phosphatase activity has been described for microorganisms, including L. johnsonii [14] and strains of L. reuteri [15]. Relative abundance of L. johnsonii ranged from 34-53% and depended on acidi cation and Ca level (Table S12).…”

Juçara (Euterpe edulis) pulp as a substrate for probiotic bacteria fermentation: Optimisation process and antioxidant activity

“…The rhamnose present after 16 hours of fermentation may have originated from the hydrolysis of cyanidin-3-rhamnoside. According to De Brito et al (2007), this anthocyanin is found at a concentration of 7 mg/100 g (db) in juçara (E. edulis), and some types of Lactic Acid Bacteria, such as Lactobacillus (including L. reuteri), Streptococcus, Enterococcus, and Weissella; which possessed β-glucosidase activity (Otieno et al, 2005, Hayek, 2013 directly acting on the anthocyanin, forming aglycones with higher antioxidant capacities compared to the glycosylated forms (Spagna et al, 2000).…”