Enzymatic activity of three sugarcane varieties under salt stress

Abstract

A B S T R A C TSugarcane is a species of great economic importance to Brazil. The study of enzymatic activity has helped to identify varieties of sugarcane tolerant to salt stress, for planting in Brazilian semiarid regions, where there is the occurrence of saline soils. The objective of this study was to evaluate the enzymatic activity of three varieties of sugarcane subjected to stress caused by salinity. The experiment was conducted in a greenhouse at the Federal Rural University of Pernambuco, in a completely randomized design with four replicates. The leaf blade samples were collected for analysis of soluble proteins and determination of the activity of catalase and ascorbate peroxidase. The protein content was higher for RB962962 and lower for the varieties RB92579 and RB867515. However, in RB867515, there was an increase in the activity of ascorbate peroxidase and catalase, while the varieties RB92579 and RB962962 showed a reduction in the activity of these enzymes. These results indicate that the variety RB867515 has a more efficient mechanism in plant protection against the action of reactive oxygen species.Atividade enzimática de três variedades de cana-de-açúcar sob estresse salino R E S U M O A cana-de-açúcar é uma espécie de grande importância econômica para o Brasil. O estudo da atividade enzimática tem ajudado a identificar variedades de cana-de-açúcar tolerantes ao estresse salino para plantio nas regiões semiáridas brasileiras nas quais ocorre incidência de solos salinizados. Objetivou-se, neste estudo, avaliar a atividade enzimática de três variedades de cana-de-açúcar submetidas ao estresse causado pela salinidade. O experimento foi conduzido em casa de vegetação na Universidade Federal Rural de Pernambuco. Foi feito um delineamento inteiramente casualizado, com quatro repetições. Foram coletadas amostras do limbo foliar para análise das proteínas solúveis e determinação da atividade das enzimas catálase e ascorbato peroxidase. O teor de proteínas foi maior para a variedade RB962962 and menor para as variedades RB92579 e RB867515; entretanto se percebeu, na variedade RB867515, aumento da atividade da ascorbato peroxidase e da catálase enquanto que nas variedades RB92579 e RB962962 ocorreu uma redução da atividade dessas enzimas cujos resultados indicam que a variedade RB867515 apresenta um mecanismo mais eficiente na proteção da planta contra a ação de espécies reativas de oxigênio.

Increasing Sugarcane Salinity Resistance with Silicon Fertilizer by Preventing the Absorption of Na+ and Cl- and Improve Antioxidant Defense System

“…Our study found that feeding sugarcane with Si fertilizer one month before applying salt stress resulted in a decrease of 18.4% in MDA levels and an increase in the activity of CAT, APX, and SOD enzymes by 36.3%, 8%, and 29.4%, respectively, compared to the control treatment (Table 6). In a study by Carvalho et al (2016) on the effects of salinity stress on three sugarcane varieties, it was found that salinity stress increases the amount of protein, APX, and catalase (CAT) activity. The RB92579 variety was found to have a more effective mechanism in protecting the plant against reactive oxygen species (ROS).…”

Saline stress affects the growth of Saccharum complex genotypes

“…In the literature, there are several reports on sugarcane mechanisms to deal with excessive Na + . These include improved ROS sequestration capacity (Satbhai & Naik, 2014), increased ascorbate peroxidase and catalase activity (Carvalho et al, 2016), lower Na + accumulation and higher proline accumulation in leaves (Chiconato et al, 2019) and presence of genes such as sugarcane shaggy‐like kinase (SuSK) and sucrose transporter (SUT) (Poonsawat et al, 2015). In this respect, Erianthus arundinaceus , Saccharum hibrido , Saccharum officinarum , Miscanthus spp .…”

“…Salinity alters the water and nutrient uptake, resulting in decreased cell division and expansion and hence reduced vegetative growth and biomass production (Silveira et al, 2016). Although sugarcane is considered a glycophytic species with moderate sensitivity to salt stress, different responses to salinity tolerance among sugarcane genotypes occur through their high genetic variability (Carvalho et al, 2016).…”