Enzymatic acylation improves the stability and bioactivity of lutein: Protective effects of acylated lutein derivatives on L-O2 cells upon H2O2-induced oxidative stress

Tan, Xinjia; Li, Haimei; Huang, Wenjing; Ma, Wenqi; Lu, Yuyun; Yan, Rian

doi:10.1016/j.foodchem.2023.135393

Cited by 8 publications

(9 citation statements)

References 37 publications

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“…Production of excess ROS was considered one of the biomarkers of oxidative stress [ 55 ]. In this research, the zebrafish treated with 5 mM H 2 O 2 exhibited significant ROS production, which was intuitively observed through DCFH-DA dyeing and fluorescence quantification calculations ( Figure 6 A), indicating the occurrence of oxidative stress.…”

Section: Resultsmentioning

confidence: 99%

“…Specifically, 50-QUE-NCs, 140-QUE-NCs, and 360-QUE-NCs lowered its level to 101.10%, 108.83%, and 109.77%, respectively. Production of excess ROS was considered one of the biomarkers of oxidative stress [55]. In this research, the zebrafish treated with 5 mM H2O2 exhibited significant ROS production, which was intuitively observed through DCFH-DA dyeing and fluorescence Lipid peroxidation not only converts ROS into non-radical lipid breakdown products but also amplifies the damage caused by ROS through chain or chain-branching reactions [52].…”

Section: Inhibitory Effects Of Que-ncs On Ros Generation Lipid Peroxi...mentioning

confidence: 92%

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Antioxidant Effects of Quercetin Nanocrystals in Nanosuspension against Hydrogen Peroxide-Induced Oxidative Stress in a Zebrafish Model

Wang,

Xue,

Miao

2023

Pharmaceuticals

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Quercetin, a flavonoid compound rich in hydroxyl groups, possesses antioxidant properties, whereas its poor water solubility limits its bioavailability. In pursuit of addressing the water solubility of quercetin and comprehending the impact of nanocrystal particle size on antioxidant efficacy, we prepared three different-sized quercetin nanocrystals, namely small (50 nm), medium (140 nm), and large (360 nm), using a nanosuspension method in this study. Within the in vitro setting, assessments employing solubility and radical scavenging assays revealed that quercetin nanocrystals displayed superior solubility (26, 21, and 13 fold corresponding to small, medium, and large particle sizes) and antioxidant performance compared to the coarse quercetin. Furthermore, quercetin nanocrystals of three particle sizes all demonstrated significant protection effects on the survival rate of H2O2-treated zebrafish at 72 h (77.78%, 73.33%, and 66.67% for small, medium, and large particle sizes, respectively), while the coarse quercetin group exhibited a low survival rate (53.3%) similar to the H2O2-treated group (47.8%). Moreover, all quercetin nanocrystals exhibited potent antioxidant capacity on both the antioxidants and enzymatic antioxidant system in H2O2-treated zebrafish to restore zebrafish to a normal state under oxidative stress. For instance, the levels of reactive oxygen species were reduced to 101.10%, 108.83%, and 109.77% of the normal levels for small, medium, and large particle-sized quercetin nanocrystals, respectively. In conclusion, quercetin nanocrystals demonstrated enhanced solubility, robust antioxidant capacity, and protective effects in zebrafish compared to coarse quercetin.

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Section: Resultsmentioning

confidence: 99%

Section: Inhibitory Effects Of Que-ncs On Ros Generation Lipid Peroxi...mentioning

confidence: 92%

Antioxidant Effects of Quercetin Nanocrystals in Nanosuspension against Hydrogen Peroxide-Induced Oxidative Stress in a Zebrafish Model

Wang,

Xue,

Miao

2023

Pharmaceuticals

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“…The old medium was discarded and freshly prepared DMEM solution (1 mL) containing H 2 O 2 (30 µg/mL) and drug-carrying nanoparticles with the CPT contents of 20 μg/mL were added into the 24-well plates. Cells and the supernatant were collected after 24 h to assess the levels of SOD, CAT, TNF-α, IL-6, and IL-1β in accordance with the kit requirements [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing

Zhang,

Yi,

Jiang

et al. 2023

Pharmaceutics

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Acute liver injury (ALI) has the potential to compromise hepatic function rapidly, with severe cases posing a considerable threat to human health and wellbeing. Conventional treatments, such as the oral administration of antioxidants, can inadvertently lead to liver toxicity and other unwanted side effects. Mesenchymal stromal cells (MSCs) can target therapeutic agents directly to inflammatory sites owing to their homing effect, and they offer a promising avenue for the treatment of ALI. However, the efficacy and feasibility of these live cell products are hampered by challenges associated with delivery pathways and safety concerns. Therefore, in this work, MSC membranes were ingeniously harnessed as protective shells to encapsulate synthesized PLGA nanoparticle cores (PLGA/MSCs). This strategic approach enabled nanoparticles to simulate endogenous substances and yielded a core–shell nano-biomimetic structure. The biomimetic nanocarrier remarkably maintained the homing ability of MSCs to inflammatory sites. In this study, cryptotanshinone (CPT)-loaded PLGA/MSCs (CPT@PLGA/MSC) were prepared. These nanoparticles can be effectively internalized by LO2 cells. They reduced cellular oxidative stress and elevated inflammatory levels. In vivo results suggested that, after intravenous administration, CPT@PLGA/MSCs significantly reduced uptake by the reticuloendothelial system and immune recognition compared to PLGA nanoparticles without MSC membrane coatings, subsequently resulting in their targeted and enhanced accumulation in the liver. The effectiveness of CPT@PLGA/MSCs in alleviating carbon tetrachloride-induced oxidative stress and inflammation in a mouse model was unequivocally demonstrated through comprehensive histological examination and liver function tests. This study introduces a pioneering strategy with substantial potential for ALI treatment.

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Section: Bioactivity Of Luteinmentioning

confidence: 99%

“…Tan et al (2023) investigated the effect of enzymatic acylation of lutein on the stability and bioactivity of lutein. The stabilities and bioactivities of three acylated novel lutein derivatives, lutein-3-O-adipate, lutein-3 0 -O-adipate, and lutein-di-adipate, were evaluated.Notably, these compounds demonstrated improved thermal and light stability as well as a higher ability to scavenge ABTS radical cation (ABTS + ) and hydroxyl radical (•OH).…”

mentioning

confidence: 99%

A mechanistic review on growing multiple therapeutic applications of lutein and its global market research

Akkewar,

Mishra,

Kamble

et al. 2024

Phytotherapy Research

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Lutein is a naturally occurring carotenoid synthesized by plants and algae that has a beneficial effect on several biological processes and associated ailments. Its immediate application is in ophthalmology, where it significantly lowers the incidences of age‐related macular degeneration (AMD). It also has anti‐inflammatory action, treatment of diabetic retinopathy, and cataracts, and enhancement of visual contrast. To critically assess lutein biosynthesis, therapeutic applicability, and market research literature. We have discussed its theoretical frameworks, experimental evidence, limitations, as well as clinical trial results, and future research prospects. The literature for this review article was mined and compiled by collecting and analyzing articles from several databases, including ScienceDirect, Google Scholar, PubMed, Wiley Online Library, Patentscope, and ClinicalTrials.gov published until March 30, 2022. Patent publications were identified using the search terms like IC:(C07C67/56) AND EN_AB:(lutein) OR EN_TI:(lutein) OR EN_AB:(extraction) OR EN_TI:(process). According to the literature, lutein is an essential nutrient given that it cannot be synthesized in the human body and acts as an antioxidant, affecting AMD, diabetic retinopathy, Rheumatic diseases, inflammation, and cancer. Due to inadequate production and laborious extraction, lutein is expensive despite its high demand and applicability. Market research predicts a 6.3% compound annual growth rate for lutein by 2032. Optimizing lutein extraction for high yield and purity is necessary. Lutein has proven applicability in various ailments as well as cosmetics that can be developed as a candidate drug for various diseases discussed in the review.

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Enzymatic acylation improves the stability and bioactivity of lutein: Protective effects of acylated lutein derivatives on L-O2 cells upon H2O2-induced oxidative stress

Cited by 8 publications

References 37 publications

Antioxidant Effects of Quercetin Nanocrystals in Nanosuspension against Hydrogen Peroxide-Induced Oxidative Stress in a Zebrafish Model

Antioxidant Effects of Quercetin Nanocrystals in Nanosuspension against Hydrogen Peroxide-Induced Oxidative Stress in a Zebrafish Model

Targeted Therapy of Acute Liver Injury via Cryptotanshinone-Loaded Biomimetic Nanoparticles Derived from Mesenchymal Stromal Cells Driven by Homing

A mechanistic review on growing multiple therapeutic applications of lutein and its global market research

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