Enzymatic amplification detection of DNA based on “molecular beacon” biosensors

A simple hairpin DNA sensor for label-free detection of sub-attomole DNA target

“…Up to date, most of DNA detection based on hairpin DNAs has been performed by fluorescent detection [4,14,15] and electrochemical measurement [5,8,[16][17][18]. However, fluorescent labeling technique and purification process are time consuming, labor intensive and cost high, meanwhile some of the electrochemical measurements need to be labeled or feature low sensitivity.…”

A novel adenosine-based molecular beacon probe for room temperature nucleic acid rapid detection in cotton thread device

“…In recent years, nanomaterials including gold nanoparticle and carbon nanotube were employed as substrates to immobilize molecular beacons. Bockisch et al developed a novel DNA stem-loop structured probe for enzymatic detection of nucleic acid target based on the molecular beacon immobilized on the surface of microplate and electrode [2,3].…”

Electrochemical DNA sensor for simultaneous detection of genes encoding two functional enzymes involved in lignin degradation

“…Recently, electrochemical DNA biosensors have received particular attention due to their fast response, high sensitivity and low cost in environmental and microbial analysis [21][22][23][24][25]. The combination of PCR amplification and DNA sensor was also utilized to improve the detection sensitivity [26,27].…”