Enzymatic assembly of adhesive molecular networks with sequence-dependent mechanical properties inspired by mussel foot proteins

Park, Byung Min; Luo, Jiren; Sun, Fei

doi:10.1039/c8py01581c

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…6 For example, the outstanding underwater properties of marine mussel have driven a field of mussel-inspired catechol chemistry of great significance in biomaterials science and engineering. [7][8][9][10][11][12][13][14][15][16][17] Similar to mussels, ascidians are sac-like sessile marine animals that are covered with a protective membrane composed of highly crystalline cellulose nanofiber (tunicin) and proteins (tunichrome) containing 3,4,5-trihydroxyphenylalanine (TOPA) with a pyrogallol moiety (three hydroxyls bound to a benzene ring). These organisms have extraordinary biophysicochemical properties including rapid self-healing and strong adhesiveness in seawater.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the gallol-driven assembly mechanism of thermoreversible supramolecular hydrogels inspired by ascidians

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Unraveling the gallol-driven assembly mechanism of thermoreversible supramolecular hydrogels inspired by ascidians

et al. 2020

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“…47,48 At last, mussel proteins or synthetic proteins that replicate core functions of mussel-proteins have been utilized as tyrosinase substrate. 50,51,[62][63][64][65] Mussels strongly bind to underwater surfaces using Mfp. The specific mechanism has not been well established, but dopamine, a product of post-translational modifications of tyrosine, has been known to endow adhesive properties to Mfp.…”

Section: Tyrosinase-mediated Biomimetic Strategy For Hydrogel Crosslinkingmentioning

confidence: 99%

“…53 Park et al designed and synthesized elastin-like polypeptides (ELPs) whose RGD sequences and short peptide sequences from Mfp-3 and Mfp-5 were inserted. 51 With tyrosinase-mediated crosslinking, ELPs exhibited tissue adhesiveness and high cell viability. The mixture of recombinant MAP and functional polymers (e.g., phenolmodified hydrogel, decellularized ECM) was also designed to use in applications of tissue engineering.…”

Section: Tyrosinase-mediated Biomimetic Strategy For Hydrogel Crosslinkingmentioning

confidence: 99%

Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

Choi

Ahn

Kim

2021

J of Applied Polymer Sci

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Hydrogel system based on enzyme-mediated mild crosslinking reaction has been a promising approach in tissue engineering. Inspired by skin melanin synthesis and marine mussel adhesion, tyrosinase-mediated hydrogel crosslinking has been exploited as cell-friendly reactions and explicit reaction mechanisms. Hydrogel prepared by tyrosinase exhibits appealing properties as a dynamic scaffold for cell delivery and as a bioink for 3D bioprinting. Recapitulating the structure of the native extracellular matrix (ECM), innovative tyrosinase-mediated hydrogel crosslinking has now shifted to the field of translational medicine. Biomimetic hydrogel with in situ tyrosinase crosslinking can be efficiently and easily applicable to the disease model for therapeutic purposes. In this review, we will discuss a broad range of tyrosinase-mediated tissue engineering from the specific mechanism of tyrosinase reaction and designing a strategy for tyrosinase-mediated hydrogel crosslinking to dynamic applications in tissue engineering. In addition, we report the current challenges and future perspectives for the translational applications.

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“…"Ultrahigh-affinity" protein/protein interactions (PPIs) have often been used to assemble recombinant proteins into functional materials (11)(12)(13)(14)(15)(16)(17). For instance, SpyTag/SpyCatcher, a peptide/ protein pair that is originally derived from a split bacterial adhesin domain, is capable of covalently stitching together protein molecules under mild physiological conditions, which has led to the creation of a number of uncommon protein architectures and materials (Fig.…”

Section: Introductionmentioning

confidence: 99%

Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions

Dai

Park

et al. 2022

Sci. Adv.

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Engineered living materials (ELMs) are gaining traction among synthetic biologists, as their emergent properties and nonequilibrium thermodynamics make them markedly different from traditional materials. However, the aspiration to directly use living cells as building blocks to create higher-order structures or materials, with no need for chemical modification, remains elusive to synthetic biologists. Here, we report a strategy that enables the assembly of engineered Saccharomyces cerevisiae into self-propagating ELMs via ultrahigh-affinity protein/protein interactions. These yeast cells have been genetically engineered to display the protein pairs SpyTag/SpyCatcher or CL7/Im7 on their surfaces, which enable their assembly into multicellular structures capable of further growth and proliferation. The assembly process can be controlled precisely via optical tweezers or microfluidics. Moreover, incorporation of functional motifs such as super uranyl-binding protein and mussel foot proteins via genetic programming rendered these materials suitable for uranium extraction from seawater and bioadhesion, respectively, pointing to their potential in chemical separation and biomedical applications.

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Enzymatic assembly of adhesive molecular networks with sequence-dependent mechanical properties inspired by mussel foot proteins

Abstract: By inserting short peptide motifs derived from Mfp-3 and Mfp-5 into elastin-like polypeptides (ELPs), we created several recombinant protein polymers that can form adhesive hydrogels upon enzymatic oxidation.

Cited by 7 publications

References 26 publications

Unraveling the gallol-driven assembly mechanism of thermoreversible supramolecular hydrogels inspired by ascidians

Unraveling the gallol-driven assembly mechanism of thermoreversible supramolecular hydrogels inspired by ascidians

Tyrosinase‐mediated hydrogel crosslinking for tissue engineering

Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions

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