Enzymatic basis of metabolic stimulation in leucocytes during phagocytosis: The role of activated NADPH oxidase

A new way for inducing a respiratory burst in guinea pig neutrophils

“…Guinea pig polymorphonuclear leukocytes (PMN) were prepared from casein-induced peritoneal exudate as in [6]. Cells, freed from erythrocytes by hypotonic lysis, were washed 3-fold and suspended (l-4 X 10") in appropriately buffered solution.…”

Stimulation of the oxidative metabolism of polymorphonuclear leucocytes by the calcium ionophore A23187

“…The increase in oxygen uptake and shunt activity can be detected within a few seconds after the addition of phagocytosable particles [ 11. In addition, several agents thought to interact with the PMN cell surface membrane, e.q. concanavalin A [2], phorbol myristate acetate [3], phospholipase C [4], endotoxin and detergents [5] provoke these oxidative changes. Thus we and others have proposed that contact between a phagocytic particle and the surface of the PMN may generate a chemical signal or signals which in turn mediate the characteristic metabolic accompaniments of phagocytosis [ 1,6], We have previously investigated the role of CAMP as a chemical signal.…”

Further characterization of NADPH oxidase activity of human polymorphonuclear leukocytes.

“…Previously, we and others have found that granule fractions isolated from phagocytizing cells had higher NADPH oxidase activity than granule fractions isolated from resting cells (1,3,4,13). This suggested that the oxidase could be the enzyme responsible for initiation of the respiratory burst.…”

“…NADPH oxidase has been proposed as the initiating enzyme of the series of metabolic events that accompanies phagocytosis (1). These events include increases in oxygen consumption, hexose monophosphate shunt activity, and the production of both hydrogen peroxide (3)(4)(5), consistent with the enzyme being the initiator.…”